Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_280

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_279 and Pf6N2E2_280 overlap by 24 nucleotidesPf6N2E2_280 and Pf6N2E2_281 are separated by 323 nucleotides Pf6N2E2_279 - Isocitrate lyase (EC 4.1.3.1), at 345,874 to 347,199 _279 Pf6N2E2_280 - hypothetical protein, at 347,176 to 347,331 _280 Pf6N2E2_281 - Type II and III secretion system family protein, at 347,655 to 348,416 _281 Position (kb) 347 348Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 346.229 kb on + strand, within Pf6N2E2_279at 346.229 kb on + strand, within Pf6N2E2_279at 346.229 kb on + strand, within Pf6N2E2_279at 346.230 kb on - strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.388 kb on + strand, within Pf6N2E2_279at 346.389 kb on - strand, within Pf6N2E2_279at 346.389 kb on - strand, within Pf6N2E2_279at 346.389 kb on - strand, within Pf6N2E2_279at 346.480 kb on - strand, within Pf6N2E2_279at 346.670 kb on + strand, within Pf6N2E2_279at 346.728 kb on - strand, within Pf6N2E2_279at 346.736 kb on + strand, within Pf6N2E2_279at 346.737 kb on - strand, within Pf6N2E2_279at 346.737 kb on - strand, within Pf6N2E2_279at 346.737 kb on - strand, within Pf6N2E2_279at 346.841 kb on + strand, within Pf6N2E2_279at 346.910 kb on + strand, within Pf6N2E2_279at 346.910 kb on + strand, within Pf6N2E2_279at 346.910 kb on + strand, within Pf6N2E2_279at 346.911 kb on - strand, within Pf6N2E2_279at 346.911 kb on - strand, within Pf6N2E2_279at 346.911 kb on - strand, within Pf6N2E2_279at 347.205 kb on + strand, within Pf6N2E2_280at 347.205 kb on + strand, within Pf6N2E2_280at 347.219 kb on + strand, within Pf6N2E2_280at 347.220 kb on - strand, within Pf6N2E2_280at 347.266 kb on - strand, within Pf6N2E2_280at 347.329 kb on + strandat 347.330 kb on - strandat 347.371 kb on + strandat 347.399 kb on + strandat 347.399 kb on + strandat 347.399 kb on + strandat 347.400 kb on - strandat 347.400 kb on - strandat 347.400 kb on - strandat 347.400 kb on - strandat 347.403 kb on + strandat 347.403 kb on + strandat 347.403 kb on + strandat 347.403 kb on + strandat 347.404 kb on - strandat 347.611 kb on + strandat 347.713 kb on + strandat 347.713 kb on + strandat 347.713 kb on + strandat 347.714 kb on - strandat 347.826 kb on + strand, within Pf6N2E2_281at 347.827 kb on - strand, within Pf6N2E2_281at 347.827 kb on - strand, within Pf6N2E2_281at 347.827 kb on - strandat 347.911 kb on + strand, within Pf6N2E2_281at 347.912 kb on - strand, within Pf6N2E2_281at 347.912 kb on - strand, within Pf6N2E2_281at 347.962 kb on + strand, within Pf6N2E2_281at 347.962 kb on + strand, within Pf6N2E2_281at 347.962 kb on + strand, within Pf6N2E2_281at 347.962 kb on + strand, within Pf6N2E2_281at 347.963 kb on - strand, within Pf6N2E2_281at 347.963 kb on - strand, within Pf6N2E2_281at 347.963 kb on - strand, within Pf6N2E2_281at 348.165 kb on - strand, within Pf6N2E2_281

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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346,229 + Pf6N2E2_279 0.27 +0.8
346,229 + Pf6N2E2_279 0.27 +1.0
346,229 + Pf6N2E2_279 0.27 -0.6
346,230 - Pf6N2E2_279 0.27 +1.8
346,388 + Pf6N2E2_279 0.39 -0.4
346,388 + Pf6N2E2_279 0.39 +0.8
346,388 + Pf6N2E2_279 0.39 -0.2
346,388 + Pf6N2E2_279 0.39 -0.4
346,388 + Pf6N2E2_279 0.39 +0.2
346,388 + Pf6N2E2_279 0.39 +0.2
346,389 - Pf6N2E2_279 0.39 +0.8
346,389 - Pf6N2E2_279 0.39 -0.8
346,389 - Pf6N2E2_279 0.39 +0.3
346,480 - Pf6N2E2_279 0.46 -0.2
346,670 + Pf6N2E2_279 0.60 -0.2
346,728 - Pf6N2E2_279 0.64 -1.6
346,736 + Pf6N2E2_279 0.65 +0.2
346,737 - Pf6N2E2_279 0.65 -0.8
346,737 - Pf6N2E2_279 0.65 +0.8
346,737 - Pf6N2E2_279 0.65 +0.1
346,841 + Pf6N2E2_279 0.73 +0.1
346,910 + Pf6N2E2_279 0.78 +0.9
346,910 + Pf6N2E2_279 0.78 -0.8
346,910 + Pf6N2E2_279 0.78 +0.6
346,911 - Pf6N2E2_279 0.78 +0.0
346,911 - Pf6N2E2_279 0.78 +0.2
346,911 - Pf6N2E2_279 0.78 +1.5
347,205 + Pf6N2E2_280 0.19 -0.7
347,205 + Pf6N2E2_280 0.19 -1.6
347,219 + Pf6N2E2_280 0.28 +0.8
347,220 - Pf6N2E2_280 0.28 -1.2
347,266 - Pf6N2E2_280 0.58 -1.8
347,329 + +0.9
347,330 - +0.2
347,371 + -0.8
347,399 + -0.1
347,399 + -0.9
347,399 + +2.3
347,400 - +0.5
347,400 - +0.0
347,400 - +0.8
347,400 - -0.2
347,403 + -0.1
347,403 + +0.4
347,403 + +2.8
347,403 + -0.5
347,404 - +0.8
347,611 + +0.5
347,713 + -0.1
347,713 + -0.5
347,713 + -2.9
347,714 - +2.6
347,826 + Pf6N2E2_281 0.22 +0.9
347,827 - Pf6N2E2_281 0.23 -0.2
347,827 - Pf6N2E2_281 0.23 +0.6
347,827 - +0.8
347,911 + Pf6N2E2_281 0.34 -0.2
347,912 - Pf6N2E2_281 0.34 -0.6
347,912 - Pf6N2E2_281 0.34 -0.4
347,962 + Pf6N2E2_281 0.40 +0.0
347,962 + Pf6N2E2_281 0.40 +0.0
347,962 + Pf6N2E2_281 0.40 +0.6
347,962 + Pf6N2E2_281 0.40 +0.3
347,963 - Pf6N2E2_281 0.40 -1.6
347,963 - Pf6N2E2_281 0.40 -0.5
347,963 - Pf6N2E2_281 0.40 +0.0
348,165 - Pf6N2E2_281 0.67 +0.9

Or see this region's nucleotide sequence