Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2426 and Pf6N2E2_2427 are separated by 72 nucleotides Pf6N2E2_2427 and Pf6N2E2_2428 are separated by 92 nucleotides Pf6N2E2_2428 and Pf6N2E2_2429 are separated by 189 nucleotides Pf6N2E2_2429 and Pf6N2E2_2430 are separated by 17 nucleotides
Pf6N2E2_2426 - Similar to ribosomal large subunit pseudouridine synthase A, at 2,875,641 to 2,876,531
_2426
Pf6N2E2_2427 - DNA damage response gene with a putative DNA binding domain and often regulated by LexA (PA0922-like) (from data) , at 2,876,604 to 2,876,924
_2427
Pf6N2E2_2428 - Thiol-disulfide isomerase and thioredoxins, at 2,877,017 to 2,877,253
_2428
Pf6N2E2_2429 - Glutathione-regulated potassium-efflux system protein KefB, at 2,877,443 to 2,879,155
_2429
Pf6N2E2_2430 - Ammonium transporter, at 2,879,173 to 2,880,381
_2430
Position (kb)
2877
2878
2879
2880 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2876.482 kb on + strand at 2876.482 kb on + strand at 2876.482 kb on + strand at 2876.489 kb on + strand at 2876.490 kb on - strand at 2876.490 kb on - strand at 2876.542 kb on + strand at 2876.563 kb on + strand at 2876.564 kb on - strand at 2876.564 kb on - strand at 2876.564 kb on - strand at 2876.695 kb on + strand, within Pf6N2E2_2427 at 2876.695 kb on + strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.696 kb on - strand, within Pf6N2E2_2427 at 2876.704 kb on + strand, within Pf6N2E2_2427 at 2876.704 kb on + strand, within Pf6N2E2_2427 at 2876.705 kb on - strand, within Pf6N2E2_2427 at 2876.705 kb on - strand, within Pf6N2E2_2427 at 2876.705 kb on - strand, within Pf6N2E2_2427 at 2876.941 kb on + strand at 2876.993 kb on - strand at 2877.111 kb on + strand, within Pf6N2E2_2428 at 2877.111 kb on + strand, within Pf6N2E2_2428 at 2877.111 kb on + strand, within Pf6N2E2_2428 at 2877.200 kb on + strand, within Pf6N2E2_2428 at 2877.288 kb on + strand at 2877.288 kb on + strand at 2877.407 kb on + strand at 2877.421 kb on + strand at 2877.421 kb on + strand at 2877.530 kb on + strand at 2877.531 kb on - strand at 2878.224 kb on + strand, within Pf6N2E2_2429 at 2878.224 kb on + strand, within Pf6N2E2_2429 at 2878.224 kb on + strand, within Pf6N2E2_2429 at 2878.224 kb on + strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.371 kb on + strand, within Pf6N2E2_2429 at 2878.536 kb on + strand, within Pf6N2E2_2429 at 2878.536 kb on + strand, within Pf6N2E2_2429 at 2878.536 kb on + strand, within Pf6N2E2_2429 at 2878.537 kb on - strand, within Pf6N2E2_2429 at 2878.753 kb on + strand, within Pf6N2E2_2429 at 2878.923 kb on + strand, within Pf6N2E2_2429 at 2879.126 kb on + strand at 2879.126 kb on + strand at 2879.225 kb on + strand at 2879.225 kb on + strand at 2879.225 kb on + strand at 2879.225 kb on + strand at 2879.225 kb on + strand at 2879.225 kb on + strand at 2879.226 kb on - strand at 2879.226 kb on - strand at 2879.226 kb on - strand at 2879.226 kb on - strand at 2879.226 kb on - strand at 2879.226 kb on - strand at 2879.276 kb on + strand at 2879.276 kb on + strand at 2879.277 kb on - strand at 2879.620 kb on + strand, within Pf6N2E2_2430 at 2879.621 kb on - strand, within Pf6N2E2_2430 at 2879.979 kb on - strand, within Pf6N2E2_2430 at 2880.108 kb on + strand, within Pf6N2E2_2430 at 2880.108 kb on + strand, within Pf6N2E2_2430
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,876,482 + +0.9 2,876,482 + +0.9 2,876,482 + +0.7 2,876,489 + +0.9 2,876,490 - +2.9 2,876,490 - +1.8 2,876,542 + +0.3 2,876,563 + +0.3 2,876,564 - -0.9 2,876,564 - -0.7 2,876,564 - +2.1 2,876,695 + Pf6N2E2_2427 0.28 +0.2 2,876,695 + Pf6N2E2_2427 0.28 +0.4 2,876,696 - Pf6N2E2_2427 0.29 +0.1 2,876,696 - Pf6N2E2_2427 0.29 -1.5 2,876,696 - Pf6N2E2_2427 0.29 -1.1 2,876,696 - Pf6N2E2_2427 0.29 +0.9 2,876,696 - Pf6N2E2_2427 0.29 +1.9 2,876,696 - Pf6N2E2_2427 0.29 +0.3 2,876,696 - Pf6N2E2_2427 0.29 +0.9 2,876,696 - Pf6N2E2_2427 0.29 +0.1 2,876,696 - Pf6N2E2_2427 0.29 -0.0 2,876,696 - Pf6N2E2_2427 0.29 -1.5 2,876,704 + Pf6N2E2_2427 0.31 -0.6 2,876,704 + Pf6N2E2_2427 0.31 +0.6 2,876,705 - Pf6N2E2_2427 0.31 +0.3 2,876,705 - Pf6N2E2_2427 0.31 +1.1 2,876,705 - Pf6N2E2_2427 0.31 +0.9 2,876,941 + -0.6 2,876,993 - -0.0 2,877,111 + Pf6N2E2_2428 0.40 -0.5 2,877,111 + Pf6N2E2_2428 0.40 +0.2 2,877,111 + Pf6N2E2_2428 0.40 -0.0 2,877,200 + Pf6N2E2_2428 0.77 -0.3 2,877,288 + +0.3 2,877,288 + +0.5 2,877,407 + +0.1 2,877,421 + -0.0 2,877,421 + -0.0 2,877,530 + +0.9 2,877,531 - -0.6 2,878,224 + Pf6N2E2_2429 0.46 +0.9 2,878,224 + Pf6N2E2_2429 0.46 -1.5 2,878,224 + Pf6N2E2_2429 0.46 -0.7 2,878,224 + Pf6N2E2_2429 0.46 -0.7 2,878,225 - Pf6N2E2_2429 0.46 -1.1 2,878,225 - Pf6N2E2_2429 0.46 +0.9 2,878,225 - Pf6N2E2_2429 0.46 +0.9 2,878,225 - Pf6N2E2_2429 0.46 +0.9 2,878,225 - Pf6N2E2_2429 0.46 +2.2 2,878,225 - Pf6N2E2_2429 0.46 -1.1 2,878,371 + Pf6N2E2_2429 0.54 -0.1 2,878,536 + Pf6N2E2_2429 0.64 -1.9 2,878,536 + Pf6N2E2_2429 0.64 -2.1 2,878,536 + Pf6N2E2_2429 0.64 -0.1 2,878,537 - Pf6N2E2_2429 0.64 +2.4 2,878,753 + Pf6N2E2_2429 0.76 -2.1 2,878,923 + Pf6N2E2_2429 0.86 +0.9 2,879,126 + -0.5 2,879,126 + -1.1 2,879,225 + -1.5 2,879,225 + +0.4 2,879,225 + -1.6 2,879,225 + +1.3 2,879,225 + -1.1 2,879,225 + +1.7 2,879,226 - -0.1 2,879,226 - -2.1 2,879,226 - -0.1 2,879,226 - +0.2 2,879,226 - -0.7 2,879,226 - -0.1 2,879,276 + -0.0 2,879,276 + +0.9 2,879,277 - +0.3 2,879,620 + Pf6N2E2_2430 0.37 +1.4 2,879,621 - Pf6N2E2_2430 0.37 +1.3 2,879,979 - Pf6N2E2_2430 0.67 -1.3 2,880,108 + Pf6N2E2_2430 0.77 +0.6 2,880,108 + Pf6N2E2_2430 0.77 -0.5
Or see this region's nucleotide sequence