Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2429

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2426 and Pf6N2E2_2427 are separated by 72 nucleotidesPf6N2E2_2427 and Pf6N2E2_2428 are separated by 92 nucleotidesPf6N2E2_2428 and Pf6N2E2_2429 are separated by 189 nucleotidesPf6N2E2_2429 and Pf6N2E2_2430 are separated by 17 nucleotides Pf6N2E2_2426 - Similar to ribosomal large subunit pseudouridine synthase A, at 2,875,641 to 2,876,531 _2426 Pf6N2E2_2427 - DNA damage response gene with a putative DNA binding domain and often regulated by LexA (PA0922-like) (from data), at 2,876,604 to 2,876,924 _2427 Pf6N2E2_2428 - Thiol-disulfide isomerase and thioredoxins, at 2,877,017 to 2,877,253 _2428 Pf6N2E2_2429 - Glutathione-regulated potassium-efflux system protein KefB, at 2,877,443 to 2,879,155 _2429 Pf6N2E2_2430 - Ammonium transporter, at 2,879,173 to 2,880,381 _2430 Position (kb) 2877 2878 2879 2880Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2876.482 kb on + strandat 2876.482 kb on + strandat 2876.482 kb on + strandat 2876.489 kb on + strandat 2876.490 kb on - strandat 2876.490 kb on - strandat 2876.542 kb on + strandat 2876.563 kb on + strandat 2876.564 kb on - strandat 2876.564 kb on - strandat 2876.564 kb on - strandat 2876.695 kb on + strand, within Pf6N2E2_2427at 2876.695 kb on + strand, within Pf6N2E2_2427at 2876.696 kb on - strand, within Pf6N2E2_2427at 2876.696 kb on - strand, within Pf6N2E2_2427at 2876.696 kb on - strand, within Pf6N2E2_2427at 2876.696 kb on - strand, within Pf6N2E2_2427at 2876.696 kb on - strand, within Pf6N2E2_2427at 2876.696 kb on - strand, within Pf6N2E2_2427at 2876.696 kb on - strand, within Pf6N2E2_2427at 2876.696 kb on - strand, within Pf6N2E2_2427at 2876.696 kb on - strand, within Pf6N2E2_2427at 2876.696 kb on - strand, within Pf6N2E2_2427at 2876.704 kb on + strand, within Pf6N2E2_2427at 2876.704 kb on + strand, within Pf6N2E2_2427at 2876.705 kb on - strand, within Pf6N2E2_2427at 2876.705 kb on - strand, within Pf6N2E2_2427at 2876.705 kb on - strand, within Pf6N2E2_2427at 2876.941 kb on + strandat 2876.993 kb on - strandat 2877.111 kb on + strand, within Pf6N2E2_2428at 2877.111 kb on + strand, within Pf6N2E2_2428at 2877.111 kb on + strand, within Pf6N2E2_2428at 2877.200 kb on + strand, within Pf6N2E2_2428at 2877.288 kb on + strandat 2877.288 kb on + strandat 2877.407 kb on + strandat 2877.421 kb on + strandat 2877.421 kb on + strandat 2877.530 kb on + strandat 2877.531 kb on - strandat 2878.224 kb on + strand, within Pf6N2E2_2429at 2878.224 kb on + strand, within Pf6N2E2_2429at 2878.224 kb on + strand, within Pf6N2E2_2429at 2878.224 kb on + strand, within Pf6N2E2_2429at 2878.225 kb on - strand, within Pf6N2E2_2429at 2878.225 kb on - strand, within Pf6N2E2_2429at 2878.225 kb on - strand, within Pf6N2E2_2429at 2878.225 kb on - strand, within Pf6N2E2_2429at 2878.225 kb on - strand, within Pf6N2E2_2429at 2878.225 kb on - strand, within Pf6N2E2_2429at 2878.371 kb on + strand, within Pf6N2E2_2429at 2878.536 kb on + strand, within Pf6N2E2_2429at 2878.536 kb on + strand, within Pf6N2E2_2429at 2878.536 kb on + strand, within Pf6N2E2_2429at 2878.537 kb on - strand, within Pf6N2E2_2429at 2878.753 kb on + strand, within Pf6N2E2_2429at 2878.923 kb on + strand, within Pf6N2E2_2429at 2879.126 kb on + strandat 2879.126 kb on + strandat 2879.225 kb on + strandat 2879.225 kb on + strandat 2879.225 kb on + strandat 2879.225 kb on + strandat 2879.225 kb on + strandat 2879.225 kb on + strandat 2879.226 kb on - strandat 2879.226 kb on - strandat 2879.226 kb on - strandat 2879.226 kb on - strandat 2879.226 kb on - strandat 2879.226 kb on - strandat 2879.276 kb on + strandat 2879.276 kb on + strandat 2879.277 kb on - strandat 2879.620 kb on + strand, within Pf6N2E2_2430at 2879.621 kb on - strand, within Pf6N2E2_2430at 2879.979 kb on - strand, within Pf6N2E2_2430at 2880.108 kb on + strand, within Pf6N2E2_2430at 2880.108 kb on + strand, within Pf6N2E2_2430

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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2,876,482 + +0.9
2,876,482 + +0.9
2,876,482 + +0.7
2,876,489 + +0.9
2,876,490 - +2.9
2,876,490 - +1.8
2,876,542 + +0.3
2,876,563 + +0.3
2,876,564 - -0.9
2,876,564 - -0.7
2,876,564 - +2.1
2,876,695 + Pf6N2E2_2427 0.28 +0.2
2,876,695 + Pf6N2E2_2427 0.28 +0.4
2,876,696 - Pf6N2E2_2427 0.29 +0.1
2,876,696 - Pf6N2E2_2427 0.29 -1.5
2,876,696 - Pf6N2E2_2427 0.29 -1.1
2,876,696 - Pf6N2E2_2427 0.29 +0.9
2,876,696 - Pf6N2E2_2427 0.29 +1.9
2,876,696 - Pf6N2E2_2427 0.29 +0.3
2,876,696 - Pf6N2E2_2427 0.29 +0.9
2,876,696 - Pf6N2E2_2427 0.29 +0.1
2,876,696 - Pf6N2E2_2427 0.29 -0.0
2,876,696 - Pf6N2E2_2427 0.29 -1.5
2,876,704 + Pf6N2E2_2427 0.31 -0.6
2,876,704 + Pf6N2E2_2427 0.31 +0.6
2,876,705 - Pf6N2E2_2427 0.31 +0.3
2,876,705 - Pf6N2E2_2427 0.31 +1.1
2,876,705 - Pf6N2E2_2427 0.31 +0.9
2,876,941 + -0.6
2,876,993 - -0.0
2,877,111 + Pf6N2E2_2428 0.40 -0.5
2,877,111 + Pf6N2E2_2428 0.40 +0.2
2,877,111 + Pf6N2E2_2428 0.40 -0.0
2,877,200 + Pf6N2E2_2428 0.77 -0.3
2,877,288 + +0.3
2,877,288 + +0.5
2,877,407 + +0.1
2,877,421 + -0.0
2,877,421 + -0.0
2,877,530 + +0.9
2,877,531 - -0.6
2,878,224 + Pf6N2E2_2429 0.46 +0.9
2,878,224 + Pf6N2E2_2429 0.46 -1.5
2,878,224 + Pf6N2E2_2429 0.46 -0.7
2,878,224 + Pf6N2E2_2429 0.46 -0.7
2,878,225 - Pf6N2E2_2429 0.46 -1.1
2,878,225 - Pf6N2E2_2429 0.46 +0.9
2,878,225 - Pf6N2E2_2429 0.46 +0.9
2,878,225 - Pf6N2E2_2429 0.46 +0.9
2,878,225 - Pf6N2E2_2429 0.46 +2.2
2,878,225 - Pf6N2E2_2429 0.46 -1.1
2,878,371 + Pf6N2E2_2429 0.54 -0.1
2,878,536 + Pf6N2E2_2429 0.64 -1.9
2,878,536 + Pf6N2E2_2429 0.64 -2.1
2,878,536 + Pf6N2E2_2429 0.64 -0.1
2,878,537 - Pf6N2E2_2429 0.64 +2.4
2,878,753 + Pf6N2E2_2429 0.76 -2.1
2,878,923 + Pf6N2E2_2429 0.86 +0.9
2,879,126 + -0.5
2,879,126 + -1.1
2,879,225 + -1.5
2,879,225 + +0.4
2,879,225 + -1.6
2,879,225 + +1.3
2,879,225 + -1.1
2,879,225 + +1.7
2,879,226 - -0.1
2,879,226 - -2.1
2,879,226 - -0.1
2,879,226 - +0.2
2,879,226 - -0.7
2,879,226 - -0.1
2,879,276 + -0.0
2,879,276 + +0.9
2,879,277 - +0.3
2,879,620 + Pf6N2E2_2430 0.37 +1.4
2,879,621 - Pf6N2E2_2430 0.37 +1.3
2,879,979 - Pf6N2E2_2430 0.67 -1.3
2,880,108 + Pf6N2E2_2430 0.77 +0.6
2,880,108 + Pf6N2E2_2430 0.77 -0.5

Or see this region's nucleotide sequence