Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2061

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2060 and Pf6N2E2_2061 are separated by 3 nucleotidesPf6N2E2_2061 and Pf6N2E2_2062 are separated by 834 nucleotides Pf6N2E2_2060 - CobN component of cobalt chelatase involved in B12 biosynthesis, at 2,449,483 to 2,453,256 _2060 Pf6N2E2_2061 - CobW GTPase involved in cobalt insertion for B12 biosynthesis, at 2,453,260 to 2,454,324 _2061 Pf6N2E2_2062 - FIG00955561: hypothetical protein, at 2,455,159 to 2,455,347 _2062 Position (kb) 2453 2454 2455Strain fitness (log2 ratio) -2 -1 0 1at 2452.464 kb on + strand, within Pf6N2E2_2060at 2452.638 kb on - strand, within Pf6N2E2_2060at 2452.721 kb on + strand, within Pf6N2E2_2060at 2452.721 kb on + strand, within Pf6N2E2_2060at 2452.721 kb on + strand, within Pf6N2E2_2060at 2452.722 kb on - strand, within Pf6N2E2_2060at 2452.722 kb on - strand, within Pf6N2E2_2060at 2452.764 kb on - strand, within Pf6N2E2_2060at 2453.046 kb on - strandat 2453.046 kb on - strandat 2453.261 kb on + strandat 2453.459 kb on - strand, within Pf6N2E2_2061at 2453.648 kb on - strand, within Pf6N2E2_2061at 2454.021 kb on + strand, within Pf6N2E2_2061at 2454.022 kb on - strand, within Pf6N2E2_2061at 2454.022 kb on - strand, within Pf6N2E2_2061at 2454.116 kb on + strand, within Pf6N2E2_2061at 2454.116 kb on + strand, within Pf6N2E2_2061at 2454.116 kb on + strand, within Pf6N2E2_2061at 2454.117 kb on - strand, within Pf6N2E2_2061at 2454.422 kb on - strandat 2454.422 kb on - strandat 2454.422 kb on - strandat 2454.422 kb on - strandat 2454.510 kb on + strandat 2454.511 kb on - strandat 2454.595 kb on + strandat 2454.595 kb on + strandat 2454.596 kb on - strandat 2454.596 kb on - strandat 2454.635 kb on + strandat 2454.636 kb on - strandat 2454.670 kb on + strandat 2454.670 kb on + strandat 2454.670 kb on + strandat 2454.671 kb on - strandat 2454.671 kb on - strandat 2454.671 kb on - strandat 2454.671 kb on - strandat 2454.671 kb on - strandat 2454.671 kb on - strandat 2454.739 kb on + strandat 2454.739 kb on + strandat 2454.805 kb on + strandat 2454.805 kb on + strandat 2454.805 kb on + strandat 2454.805 kb on + strandat 2454.805 kb on + strandat 2454.805 kb on + strandat 2454.806 kb on - strandat 2454.806 kb on - strandat 2454.806 kb on - strandat 2454.806 kb on - strandat 2454.846 kb on + strandat 2454.846 kb on + strandat 2454.851 kb on - strandat 2454.900 kb on + strandat 2454.901 kb on - strandat 2455.194 kb on + strand, within Pf6N2E2_2062at 2455.195 kb on - strand, within Pf6N2E2_2062

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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2,452,464 + Pf6N2E2_2060 0.79 -1.3
2,452,638 - Pf6N2E2_2060 0.84 -0.3
2,452,721 + Pf6N2E2_2060 0.86 +1.1
2,452,721 + Pf6N2E2_2060 0.86 -1.3
2,452,721 + Pf6N2E2_2060 0.86 -1.3
2,452,722 - Pf6N2E2_2060 0.86 -0.9
2,452,722 - Pf6N2E2_2060 0.86 +1.5
2,452,764 - Pf6N2E2_2060 0.87 -2.6
2,453,046 - +1.1
2,453,046 - +0.7
2,453,261 + -1.0
2,453,459 - Pf6N2E2_2061 0.19 -0.9
2,453,648 - Pf6N2E2_2061 0.36 -0.3
2,454,021 + Pf6N2E2_2061 0.71 +0.3
2,454,022 - Pf6N2E2_2061 0.72 -0.3
2,454,022 - Pf6N2E2_2061 0.72 +0.7
2,454,116 + Pf6N2E2_2061 0.80 -2.4
2,454,116 + Pf6N2E2_2061 0.80 -1.3
2,454,116 + Pf6N2E2_2061 0.80 -0.3
2,454,117 - Pf6N2E2_2061 0.80 -0.3
2,454,422 - +0.3
2,454,422 - -0.9
2,454,422 - +0.7
2,454,422 - +0.1
2,454,510 + -0.3
2,454,511 - -0.1
2,454,595 + -0.9
2,454,595 + -0.3
2,454,596 - +0.7
2,454,596 - -1.1
2,454,635 + -0.9
2,454,636 - -1.1
2,454,670 + +0.9
2,454,670 + +0.7
2,454,670 + +0.5
2,454,671 - +0.1
2,454,671 - +0.7
2,454,671 - +1.1
2,454,671 - -0.8
2,454,671 - -0.3
2,454,671 - +1.2
2,454,739 + +1.0
2,454,739 + -2.3
2,454,805 + -0.3
2,454,805 + -1.3
2,454,805 + -0.4
2,454,805 + -0.1
2,454,805 + -0.7
2,454,805 + +0.3
2,454,806 - -0.0
2,454,806 - +0.4
2,454,806 - -0.3
2,454,806 - -0.0
2,454,846 + -0.0
2,454,846 + -0.3
2,454,851 - -1.1
2,454,900 + -1.3
2,454,901 - -0.4
2,455,194 + Pf6N2E2_2062 0.19 -0.0
2,455,195 - Pf6N2E2_2062 0.19 -0.1

Or see this region's nucleotide sequence