Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1837 and Pf6N2E2_1838 are separated by 10 nucleotides Pf6N2E2_1838 and Pf6N2E2_1839 overlap by 1 nucleotides Pf6N2E2_1839 and Pf6N2E2_1840 overlap by 4 nucleotides Pf6N2E2_1840 and Pf6N2E2_1841 are separated by 10 nucleotides
Pf6N2E2_1837 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10), at 2,175,429 to 2,176,907
_1837
Pf6N2E2_1838 - 6-hexanolactone hydrolase, at 2,176,918 to 2,177,823
_1838
Pf6N2E2_1839 - 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (EC 1.1.1.-), at 2,177,823 to 2,178,557
_1839
Pf6N2E2_1840 - Enoyl-CoA hydratase (EC 4.2.1.17), at 2,178,554 to 2,179,366
_1840
Pf6N2E2_1841 - hypothetical protein, at 2,179,377 to 2,179,526
_1841
Position (kb)
2177
2178
2179 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2176.877 kb on - strand at 2176.924 kb on + strand at 2176.925 kb on - strand at 2177.029 kb on + strand, within Pf6N2E2_1838 at 2177.201 kb on + strand, within Pf6N2E2_1838 at 2177.267 kb on + strand, within Pf6N2E2_1838 at 2177.456 kb on - strand, within Pf6N2E2_1838 at 2177.456 kb on - strand, within Pf6N2E2_1838 at 2177.603 kb on + strand, within Pf6N2E2_1838 at 2177.792 kb on - strand at 2178.115 kb on + strand, within Pf6N2E2_1839 at 2178.225 kb on + strand, within Pf6N2E2_1839 at 2178.254 kb on - strand, within Pf6N2E2_1839 at 2178.254 kb on - strand, within Pf6N2E2_1839 at 2178.302 kb on - strand, within Pf6N2E2_1839 at 2178.435 kb on - strand, within Pf6N2E2_1839 at 2178.500 kb on + strand at 2178.707 kb on - strand, within Pf6N2E2_1840 at 2179.087 kb on - strand, within Pf6N2E2_1840 at 2179.087 kb on - strand, within Pf6N2E2_1840 at 2179.105 kb on + strand, within Pf6N2E2_1840 at 2179.107 kb on + strand, within Pf6N2E2_1840 at 2179.108 kb on - strand, within Pf6N2E2_1840 at 2179.108 kb on - strand, within Pf6N2E2_1840 at 2179.141 kb on + strand, within Pf6N2E2_1840 at 2179.141 kb on + strand, within Pf6N2E2_1840 at 2179.142 kb on - strand, within Pf6N2E2_1840
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,176,877 - +2.3 2,176,924 + -1.6 2,176,925 - -0.3 2,177,029 + Pf6N2E2_1838 0.12 +1.3 2,177,201 + Pf6N2E2_1838 0.31 -0.4 2,177,267 + Pf6N2E2_1838 0.39 +0.7 2,177,456 - Pf6N2E2_1838 0.59 +1.4 2,177,456 - Pf6N2E2_1838 0.59 +1.2 2,177,603 + Pf6N2E2_1838 0.76 +1.1 2,177,792 - -2.5 2,178,115 + Pf6N2E2_1839 0.40 -0.7 2,178,225 + Pf6N2E2_1839 0.55 -0.3 2,178,254 - Pf6N2E2_1839 0.59 +0.1 2,178,254 - Pf6N2E2_1839 0.59 -0.9 2,178,302 - Pf6N2E2_1839 0.65 +0.7 2,178,435 - Pf6N2E2_1839 0.83 -0.6 2,178,500 + -1.3 2,178,707 - Pf6N2E2_1840 0.19 +0.6 2,179,087 - Pf6N2E2_1840 0.66 +0.5 2,179,087 - Pf6N2E2_1840 0.66 -0.0 2,179,105 + Pf6N2E2_1840 0.68 +0.8 2,179,107 + Pf6N2E2_1840 0.68 -0.9 2,179,108 - Pf6N2E2_1840 0.68 +0.7 2,179,108 - Pf6N2E2_1840 0.68 -1.9 2,179,141 + Pf6N2E2_1840 0.72 +0.6 2,179,141 + Pf6N2E2_1840 0.72 -0.1 2,179,142 - Pf6N2E2_1840 0.72 +1.7
Or see this region's nucleotide sequence