Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1750 and Pf6N2E2_1751 are separated by 64 nucleotides Pf6N2E2_1751 and Pf6N2E2_1752 are separated by 62 nucleotides
Pf6N2E2_1750 - Sigma-54 dependent transcriptional regulator, at 2,063,866 to 2,065,788
_1750
Pf6N2E2_1751 - Aldehyde dehydrogenase A (EC 1.2.1.22) / Glycolaldehyde dehydrogenase (EC 1.2.1.21), at 2,065,853 to 2,067,286
_1751
Pf6N2E2_1752 - Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8), at 2,067,349 to 2,069,529
_1752
Position (kb)
2065
2066
2067
2068 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2065.092 kb on + strand, within Pf6N2E2_1750 at 2065.308 kb on + strand, within Pf6N2E2_1750 at 2065.308 kb on + strand, within Pf6N2E2_1750 at 2065.309 kb on - strand, within Pf6N2E2_1750 at 2065.309 kb on - strand, within Pf6N2E2_1750 at 2065.610 kb on - strand at 2065.866 kb on + strand at 2065.866 kb on + strand at 2065.866 kb on + strand at 2065.866 kb on + strand at 2065.867 kb on - strand at 2065.893 kb on + strand at 2065.985 kb on - strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.986 kb on + strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2065.987 kb on - strand at 2066.047 kb on - strand, within Pf6N2E2_1751 at 2066.243 kb on - strand, within Pf6N2E2_1751 at 2066.782 kb on + strand, within Pf6N2E2_1751 at 2066.955 kb on + strand, within Pf6N2E2_1751 at 2066.955 kb on + strand, within Pf6N2E2_1751 at 2066.955 kb on + strand, within Pf6N2E2_1751 at 2066.956 kb on - strand, within Pf6N2E2_1751 at 2066.956 kb on - strand, within Pf6N2E2_1751 at 2067.084 kb on + strand, within Pf6N2E2_1751 at 2067.085 kb on - strand, within Pf6N2E2_1751 at 2067.261 kb on + strand at 2067.270 kb on + strand at 2067.271 kb on - strand at 2067.350 kb on + strand at 2067.350 kb on + strand at 2067.351 kb on - strand at 2067.419 kb on + strand at 2067.420 kb on - strand at 2067.420 kb on - strand at 2067.451 kb on - strand at 2067.608 kb on + strand, within Pf6N2E2_1752 at 2067.608 kb on + strand, within Pf6N2E2_1752 at 2067.720 kb on - strand, within Pf6N2E2_1752 at 2067.720 kb on - strand, within Pf6N2E2_1752 at 2067.818 kb on + strand, within Pf6N2E2_1752 at 2067.839 kb on + strand, within Pf6N2E2_1752 at 2067.839 kb on + strand, within Pf6N2E2_1752 at 2067.840 kb on - strand, within Pf6N2E2_1752 at 2067.840 kb on - strand, within Pf6N2E2_1752 at 2067.840 kb on - strand, within Pf6N2E2_1752 at 2067.991 kb on + strand, within Pf6N2E2_1752 at 2067.991 kb on + strand, within Pf6N2E2_1752 at 2067.992 kb on - strand, within Pf6N2E2_1752 at 2068.124 kb on + strand, within Pf6N2E2_1752 at 2068.125 kb on - strand, within Pf6N2E2_1752 at 2068.125 kb on - strand, within Pf6N2E2_1752 at 2068.173 kb on - strand, within Pf6N2E2_1752 at 2068.200 kb on + strand, within Pf6N2E2_1752 at 2068.202 kb on + strand, within Pf6N2E2_1752 at 2068.202 kb on + strand, within Pf6N2E2_1752 at 2068.203 kb on - strand, within Pf6N2E2_1752 at 2068.226 kb on + strand, within Pf6N2E2_1752 at 2068.226 kb on + strand, within Pf6N2E2_1752
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,065,092 + Pf6N2E2_1750 0.64 +2.1 2,065,308 + Pf6N2E2_1750 0.75 -0.3 2,065,308 + Pf6N2E2_1750 0.75 +1.7 2,065,309 - Pf6N2E2_1750 0.75 -0.3 2,065,309 - Pf6N2E2_1750 0.75 -1.2 2,065,610 - +0.9 2,065,866 + +0.7 2,065,866 + +1.3 2,065,866 + -1.0 2,065,866 + -1.5 2,065,867 - +0.1 2,065,893 + +1.7 2,065,985 - +1.3 2,065,986 + +0.7 2,065,986 + -0.6 2,065,986 + +1.5 2,065,986 + -0.9 2,065,986 + +0.7 2,065,987 - +0.7 2,065,987 - +0.1 2,065,987 - -0.0 2,065,987 - -0.9 2,065,987 - +0.7 2,065,987 - -0.2 2,065,987 - +0.0 2,065,987 - -2.0 2,066,047 - Pf6N2E2_1751 0.14 -1.1 2,066,243 - Pf6N2E2_1751 0.27 -0.4 2,066,782 + Pf6N2E2_1751 0.65 -0.5 2,066,955 + Pf6N2E2_1751 0.77 -1.1 2,066,955 + Pf6N2E2_1751 0.77 +0.4 2,066,955 + Pf6N2E2_1751 0.77 +0.7 2,066,956 - Pf6N2E2_1751 0.77 -1.5 2,066,956 - Pf6N2E2_1751 0.77 +0.2 2,067,084 + Pf6N2E2_1751 0.86 -1.1 2,067,085 - Pf6N2E2_1751 0.86 -0.3 2,067,261 + +0.7 2,067,270 + +0.7 2,067,271 - -1.5 2,067,350 + +0.1 2,067,350 + +0.5 2,067,351 - +0.5 2,067,419 + -1.2 2,067,420 - -0.6 2,067,420 - +0.5 2,067,451 - +0.7 2,067,608 + Pf6N2E2_1752 0.12 -0.3 2,067,608 + Pf6N2E2_1752 0.12 -1.3 2,067,720 - Pf6N2E2_1752 0.17 +1.7 2,067,720 - Pf6N2E2_1752 0.17 -0.6 2,067,818 + Pf6N2E2_1752 0.22 +0.6 2,067,839 + Pf6N2E2_1752 0.22 -1.0 2,067,839 + Pf6N2E2_1752 0.22 -0.6 2,067,840 - Pf6N2E2_1752 0.23 +1.3 2,067,840 - Pf6N2E2_1752 0.23 +0.7 2,067,840 - Pf6N2E2_1752 0.23 +0.7 2,067,991 + Pf6N2E2_1752 0.29 +1.0 2,067,991 + Pf6N2E2_1752 0.29 +0.5 2,067,992 - Pf6N2E2_1752 0.29 -1.4 2,068,124 + Pf6N2E2_1752 0.36 -0.2 2,068,125 - Pf6N2E2_1752 0.36 +0.1 2,068,125 - Pf6N2E2_1752 0.36 +2.0 2,068,173 - Pf6N2E2_1752 0.38 +1.7 2,068,200 + Pf6N2E2_1752 0.39 -0.8 2,068,202 + Pf6N2E2_1752 0.39 +0.9 2,068,202 + Pf6N2E2_1752 0.39 -0.9 2,068,203 - Pf6N2E2_1752 0.39 -0.6 2,068,226 + Pf6N2E2_1752 0.40 +0.7 2,068,226 + Pf6N2E2_1752 0.40 -0.5
Or see this region's nucleotide sequence