Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1653

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1651 and Pf6N2E2_1652 are separated by 48 nucleotidesPf6N2E2_1652 and Pf6N2E2_1653 are separated by 169 nucleotidesPf6N2E2_1653 and Pf6N2E2_1654 are separated by 76 nucleotidesPf6N2E2_1654 and Pf6N2E2_1655 are separated by 99 nucleotides Pf6N2E2_1651 - Sucrose specific transcriptional regulator CscR, LacI family, at 1,957,429 to 1,958,412 _1651 Pf6N2E2_1652 - Transcriptional regulator, TetR family, at 1,958,461 to 1,959,048 _1652 Pf6N2E2_1653 - NADH:flavin oxidoreductase/NADH oxidase, at 1,959,218 to 1,960,312 _1653 Pf6N2E2_1654 - Oxidoreductase, short chain dehydrogenase/reductase family, at 1,960,389 to 1,961,177 _1654 Pf6N2E2_1655 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 1,961,277 to 1,962,293 _1655 Position (kb) 1959 1960 1961Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1958.410 kb on + strandat 1958.410 kb on + strandat 1958.410 kb on + strandat 1958.411 kb on - strandat 1958.439 kb on + strandat 1958.440 kb on - strandat 1958.462 kb on + strandat 1958.462 kb on + strandat 1958.796 kb on - strand, within Pf6N2E2_1652at 1958.796 kb on - strand, within Pf6N2E2_1652at 1958.796 kb on - strand, within Pf6N2E2_1652at 1958.816 kb on - strand, within Pf6N2E2_1652at 1959.245 kb on + strandat 1959.245 kb on + strandat 1959.246 kb on - strandat 1959.302 kb on - strandat 1959.347 kb on + strand, within Pf6N2E2_1653at 1959.348 kb on - strand, within Pf6N2E2_1653at 1959.350 kb on + strand, within Pf6N2E2_1653at 1959.351 kb on - strand, within Pf6N2E2_1653at 1959.420 kb on - strand, within Pf6N2E2_1653at 1959.440 kb on + strand, within Pf6N2E2_1653at 1959.610 kb on - strand, within Pf6N2E2_1653at 1959.736 kb on - strand, within Pf6N2E2_1653at 1960.088 kb on + strand, within Pf6N2E2_1653at 1960.089 kb on - strand, within Pf6N2E2_1653at 1960.089 kb on - strand, within Pf6N2E2_1653at 1960.106 kb on + strand, within Pf6N2E2_1653at 1960.107 kb on - strand, within Pf6N2E2_1653at 1960.250 kb on + strandat 1960.251 kb on - strandat 1960.271 kb on + strandat 1960.342 kb on + strandat 1960.446 kb on + strandat 1960.560 kb on - strand, within Pf6N2E2_1654at 1960.724 kb on + strand, within Pf6N2E2_1654at 1960.846 kb on - strand, within Pf6N2E2_1654at 1960.917 kb on + strand, within Pf6N2E2_1654at 1960.921 kb on + strand, within Pf6N2E2_1654at 1960.921 kb on + strand, within Pf6N2E2_1654at 1961.261 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,958,410 + +0.6
1,958,410 + +0.6
1,958,410 + -1.7
1,958,411 - +0.6
1,958,439 + -1.0
1,958,440 - +0.6
1,958,462 + -0.2
1,958,462 + -2.2
1,958,796 - Pf6N2E2_1652 0.57 -0.2
1,958,796 - Pf6N2E2_1652 0.57 -0.9
1,958,796 - Pf6N2E2_1652 0.57 +0.6
1,958,816 - Pf6N2E2_1652 0.60 +0.6
1,959,245 + +0.5
1,959,245 + +0.4
1,959,246 - -0.5
1,959,302 - -0.2
1,959,347 + Pf6N2E2_1653 0.12 -3.6
1,959,348 - Pf6N2E2_1653 0.12 +1.9
1,959,350 + Pf6N2E2_1653 0.12 -0.6
1,959,351 - Pf6N2E2_1653 0.12 +0.1
1,959,420 - Pf6N2E2_1653 0.18 +1.2
1,959,440 + Pf6N2E2_1653 0.20 -2.0
1,959,610 - Pf6N2E2_1653 0.36 -1.2
1,959,736 - Pf6N2E2_1653 0.47 +0.7
1,960,088 + Pf6N2E2_1653 0.79 -0.1
1,960,089 - Pf6N2E2_1653 0.80 -1.4
1,960,089 - Pf6N2E2_1653 0.80 -0.4
1,960,106 + Pf6N2E2_1653 0.81 -0.9
1,960,107 - Pf6N2E2_1653 0.81 -1.2
1,960,250 + +0.8
1,960,251 - -0.4
1,960,271 + +0.2
1,960,342 + +0.0
1,960,446 + +0.9
1,960,560 - Pf6N2E2_1654 0.22 +0.6
1,960,724 + Pf6N2E2_1654 0.42 -0.7
1,960,846 - Pf6N2E2_1654 0.58 -0.1
1,960,917 + Pf6N2E2_1654 0.67 -1.0
1,960,921 + Pf6N2E2_1654 0.67 +1.0
1,960,921 + Pf6N2E2_1654 0.67 +0.2
1,961,261 - +1.4

Or see this region's nucleotide sequence