Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1632 and Pf6N2E2_1633 are separated by 83 nucleotides Pf6N2E2_1633 and Pf6N2E2_1634 overlap by 4 nucleotides Pf6N2E2_1634 and Pf6N2E2_1635 are separated by 14 nucleotides
Pf6N2E2_1632 - Iron(III) dicitrate transport system, periplasmic iron-binding protein FecB (TC 3.A.1.14.1), at 1,931,621 to 1,932,529
_1632
Pf6N2E2_1633 - Iron(III) dicitrate transport system permease protein FecD (TC 3.A.1.14.1), at 1,932,613 to 1,933,662
_1633
Pf6N2E2_1634 - ABC-type Fe3+-siderophore transport system, permease component, at 1,933,659 to 1,934,624
_1634
Pf6N2E2_1635 - ABC-type cobalamin/Fe3+-siderophores transport systems ATPase components, at 1,934,639 to 1,935,442
_1635
Position (kb)
1932
1933
1934 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1931.623 kb on - strand at 1931.623 kb on - strand at 1931.701 kb on + strand at 1931.823 kb on + strand, within Pf6N2E2_1632 at 1931.823 kb on + strand, within Pf6N2E2_1632 at 1931.824 kb on - strand, within Pf6N2E2_1632 at 1931.824 kb on - strand, within Pf6N2E2_1632 at 1931.824 kb on - strand, within Pf6N2E2_1632 at 1931.824 kb on - strand, within Pf6N2E2_1632 at 1931.824 kb on - strand, within Pf6N2E2_1632 at 1931.875 kb on - strand, within Pf6N2E2_1632 at 1932.078 kb on + strand, within Pf6N2E2_1632 at 1932.120 kb on + strand, within Pf6N2E2_1632 at 1932.121 kb on - strand, within Pf6N2E2_1632 at 1932.121 kb on - strand, within Pf6N2E2_1632 at 1932.202 kb on - strand, within Pf6N2E2_1632 at 1932.498 kb on - strand at 1932.503 kb on + strand at 1932.503 kb on + strand at 1932.524 kb on + strand at 1932.524 kb on + strand at 1932.524 kb on + strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1932.525 kb on - strand at 1933.007 kb on - strand, within Pf6N2E2_1633 at 1933.023 kb on + strand, within Pf6N2E2_1633 at 1933.104 kb on - strand, within Pf6N2E2_1633 at 1933.254 kb on - strand, within Pf6N2E2_1633 at 1933.290 kb on + strand, within Pf6N2E2_1633 at 1933.524 kb on - strand, within Pf6N2E2_1633 at 1934.053 kb on + strand, within Pf6N2E2_1634 at 1934.053 kb on + strand, within Pf6N2E2_1634 at 1934.053 kb on + strand, within Pf6N2E2_1634 at 1934.054 kb on - strand, within Pf6N2E2_1634 at 1934.163 kb on - strand, within Pf6N2E2_1634 at 1934.163 kb on - strand, within Pf6N2E2_1634 at 1934.163 kb on - strand, within Pf6N2E2_1634 at 1934.300 kb on + strand, within Pf6N2E2_1634 at 1934.301 kb on - strand, within Pf6N2E2_1634 at 1934.301 kb on - strand, within Pf6N2E2_1634 at 1934.513 kb on - strand, within Pf6N2E2_1634 at 1934.513 kb on - strand, within Pf6N2E2_1634 at 1934.547 kb on + strand at 1934.547 kb on + strand at 1934.547 kb on + strand at 1934.547 kb on + strand at 1934.548 kb on - strand at 1934.548 kb on - strand at 1934.548 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,931,623 - +0.6 1,931,623 - +1.1 1,931,701 + +0.0 1,931,823 + Pf6N2E2_1632 0.22 +0.6 1,931,823 + Pf6N2E2_1632 0.22 -0.4 1,931,824 - Pf6N2E2_1632 0.22 +0.6 1,931,824 - Pf6N2E2_1632 0.22 +0.0 1,931,824 - Pf6N2E2_1632 0.22 -1.7 1,931,824 - Pf6N2E2_1632 0.22 +0.6 1,931,824 - Pf6N2E2_1632 0.22 -1.7 1,931,875 - Pf6N2E2_1632 0.28 -0.7 1,932,078 + Pf6N2E2_1632 0.50 -1.6 1,932,120 + Pf6N2E2_1632 0.55 +0.6 1,932,121 - Pf6N2E2_1632 0.55 -0.7 1,932,121 - Pf6N2E2_1632 0.55 -1.6 1,932,202 - Pf6N2E2_1632 0.64 +1.0 1,932,498 - -1.0 1,932,503 + +0.6 1,932,503 + -3.0 1,932,524 + +0.6 1,932,524 + -2.4 1,932,524 + -1.0 1,932,525 - -0.6 1,932,525 - -1.1 1,932,525 - -0.4 1,932,525 - -0.4 1,932,525 - -2.6 1,932,525 - -1.8 1,932,525 - -3.2 1,932,525 - -1.5 1,932,525 - -1.0 1,932,525 - +1.6 1,932,525 - -1.1 1,932,525 - -1.8 1,932,525 - -2.8 1,932,525 - -1.7 1,932,525 - -0.7 1,932,525 - -1.7 1,932,525 - -1.7 1,933,007 - Pf6N2E2_1633 0.38 -2.4 1,933,023 + Pf6N2E2_1633 0.39 +0.9 1,933,104 - Pf6N2E2_1633 0.47 -1.6 1,933,254 - Pf6N2E2_1633 0.61 -1.1 1,933,290 + Pf6N2E2_1633 0.64 +0.0 1,933,524 - Pf6N2E2_1633 0.87 -1.6 1,934,053 + Pf6N2E2_1634 0.41 +0.0 1,934,053 + Pf6N2E2_1634 0.41 -1.7 1,934,053 + Pf6N2E2_1634 0.41 +0.6 1,934,054 - Pf6N2E2_1634 0.41 +0.1 1,934,163 - Pf6N2E2_1634 0.52 +0.6 1,934,163 - Pf6N2E2_1634 0.52 -3.1 1,934,163 - Pf6N2E2_1634 0.52 -1.4 1,934,300 + Pf6N2E2_1634 0.66 -1.2 1,934,301 - Pf6N2E2_1634 0.66 -2.2 1,934,301 - Pf6N2E2_1634 0.66 -1.0 1,934,513 - Pf6N2E2_1634 0.88 -1.6 1,934,513 - Pf6N2E2_1634 0.88 -1.6 1,934,547 + -0.4 1,934,547 + -3.3 1,934,547 + -2.1 1,934,547 + -1.4 1,934,548 - +0.6 1,934,548 - +0.2 1,934,548 - +0.4
Or see this region's nucleotide sequence