Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1630 and Pf6N2E2_1631 are separated by 70 nucleotides Pf6N2E2_1631 and Pf6N2E2_1632 are separated by 119 nucleotides
Pf6N2E2_1630 - COG1020: Non-ribosomal peptide synthetase modules and related proteins, at 1,925,624 to 1,930,441
_1630
Pf6N2E2_1631 - SyrP-like protein, at 1,930,512 to 1,931,501
_1631
Pf6N2E2_1632 - Iron(III) dicitrate transport system, periplasmic iron-binding protein FecB (TC 3.A.1.14.1), at 1,931,621 to 1,932,529
_1632
Position (kb)
1930
1931
1932 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1929.528 kb on + strand, within Pf6N2E2_1630 at 1929.529 kb on - strand, within Pf6N2E2_1630 at 1929.529 kb on - strand, within Pf6N2E2_1630 at 1929.577 kb on + strand, within Pf6N2E2_1630 at 1929.745 kb on - strand, within Pf6N2E2_1630 at 1929.745 kb on - strand, within Pf6N2E2_1630 at 1929.822 kb on + strand, within Pf6N2E2_1630 at 1929.822 kb on + strand, within Pf6N2E2_1630 at 1929.822 kb on + strand, within Pf6N2E2_1630 at 1929.822 kb on + strand, within Pf6N2E2_1630 at 1929.823 kb on - strand, within Pf6N2E2_1630 at 1929.823 kb on - strand, within Pf6N2E2_1630 at 1929.823 kb on - strand, within Pf6N2E2_1630 at 1929.823 kb on - strand, within Pf6N2E2_1630 at 1929.864 kb on - strand, within Pf6N2E2_1630 at 1929.967 kb on + strand at 1929.968 kb on - strand at 1929.968 kb on - strand at 1930.191 kb on + strand at 1930.191 kb on + strand at 1930.192 kb on - strand at 1930.192 kb on - strand at 1930.458 kb on + strand at 1930.458 kb on + strand at 1930.458 kb on + strand at 1930.459 kb on - strand at 1930.471 kb on + strand at 1930.787 kb on + strand, within Pf6N2E2_1631 at 1930.788 kb on - strand, within Pf6N2E2_1631 at 1930.991 kb on - strand, within Pf6N2E2_1631 at 1931.002 kb on + strand, within Pf6N2E2_1631 at 1931.003 kb on - strand, within Pf6N2E2_1631 at 1931.111 kb on + strand, within Pf6N2E2_1631 at 1931.111 kb on + strand, within Pf6N2E2_1631 at 1931.111 kb on + strand, within Pf6N2E2_1631 at 1931.112 kb on - strand, within Pf6N2E2_1631 at 1931.112 kb on - strand, within Pf6N2E2_1631 at 1931.207 kb on - strand, within Pf6N2E2_1631 at 1931.207 kb on - strand, within Pf6N2E2_1631 at 1931.207 kb on - strand, within Pf6N2E2_1631 at 1931.209 kb on - strand, within Pf6N2E2_1631 at 1931.252 kb on - strand, within Pf6N2E2_1631 at 1931.252 kb on - strand, within Pf6N2E2_1631 at 1931.277 kb on - strand, within Pf6N2E2_1631 at 1931.361 kb on + strand, within Pf6N2E2_1631 at 1931.362 kb on - strand, within Pf6N2E2_1631 at 1931.425 kb on - strand at 1931.503 kb on + strand at 1931.504 kb on - strand at 1931.504 kb on - strand at 1931.529 kb on + strand at 1931.545 kb on - strand at 1931.604 kb on - strand at 1931.623 kb on - strand at 1931.623 kb on - strand at 1931.701 kb on + strand at 1931.823 kb on + strand, within Pf6N2E2_1632 at 1931.823 kb on + strand, within Pf6N2E2_1632 at 1931.824 kb on - strand, within Pf6N2E2_1632 at 1931.824 kb on - strand, within Pf6N2E2_1632 at 1931.824 kb on - strand, within Pf6N2E2_1632 at 1931.824 kb on - strand, within Pf6N2E2_1632 at 1931.824 kb on - strand, within Pf6N2E2_1632 at 1931.875 kb on - strand, within Pf6N2E2_1632 at 1932.078 kb on + strand, within Pf6N2E2_1632 at 1932.120 kb on + strand, within Pf6N2E2_1632 at 1932.121 kb on - strand, within Pf6N2E2_1632 at 1932.121 kb on - strand, within Pf6N2E2_1632 at 1932.202 kb on - strand, within Pf6N2E2_1632 at 1932.498 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,929,528 + Pf6N2E2_1630 0.81 +0.6 1,929,529 - Pf6N2E2_1630 0.81 -1.0 1,929,529 - Pf6N2E2_1630 0.81 +1.4 1,929,577 + Pf6N2E2_1630 0.82 +1.2 1,929,745 - Pf6N2E2_1630 0.86 +0.4 1,929,745 - Pf6N2E2_1630 0.86 +1.8 1,929,822 + Pf6N2E2_1630 0.87 -1.0 1,929,822 + Pf6N2E2_1630 0.87 +0.3 1,929,822 + Pf6N2E2_1630 0.87 +0.6 1,929,822 + Pf6N2E2_1630 0.87 -0.8 1,929,823 - Pf6N2E2_1630 0.87 +0.3 1,929,823 - Pf6N2E2_1630 0.87 -0.5 1,929,823 - Pf6N2E2_1630 0.87 +2.2 1,929,823 - Pf6N2E2_1630 0.87 +0.6 1,929,864 - Pf6N2E2_1630 0.88 +0.4 1,929,967 + -0.4 1,929,968 - +2.4 1,929,968 - +1.2 1,930,191 + +0.4 1,930,191 + -0.7 1,930,192 - +0.3 1,930,192 - -1.0 1,930,458 + +0.9 1,930,458 + +0.4 1,930,458 + -1.2 1,930,459 - -1.0 1,930,471 + +0.6 1,930,787 + Pf6N2E2_1631 0.28 +0.6 1,930,788 - Pf6N2E2_1631 0.28 -1.4 1,930,991 - Pf6N2E2_1631 0.48 -0.9 1,931,002 + Pf6N2E2_1631 0.49 -2.4 1,931,003 - Pf6N2E2_1631 0.50 +2.2 1,931,111 + Pf6N2E2_1631 0.61 -0.7 1,931,111 + Pf6N2E2_1631 0.61 +0.3 1,931,111 + Pf6N2E2_1631 0.61 -0.7 1,931,112 - Pf6N2E2_1631 0.61 -0.8 1,931,112 - Pf6N2E2_1631 0.61 -0.1 1,931,207 - Pf6N2E2_1631 0.70 -0.7 1,931,207 - Pf6N2E2_1631 0.70 -1.2 1,931,207 - Pf6N2E2_1631 0.70 +1.2 1,931,209 - Pf6N2E2_1631 0.70 +0.3 1,931,252 - Pf6N2E2_1631 0.75 -0.7 1,931,252 - Pf6N2E2_1631 0.75 -0.3 1,931,277 - Pf6N2E2_1631 0.77 -0.4 1,931,361 + Pf6N2E2_1631 0.86 -1.7 1,931,362 - Pf6N2E2_1631 0.86 -1.8 1,931,425 - -1.1 1,931,503 + -1.4 1,931,504 - +0.2 1,931,504 - -1.5 1,931,529 + +0.6 1,931,545 - -0.4 1,931,604 - +0.6 1,931,623 - +0.6 1,931,623 - +1.1 1,931,701 + +0.0 1,931,823 + Pf6N2E2_1632 0.22 +0.6 1,931,823 + Pf6N2E2_1632 0.22 -0.4 1,931,824 - Pf6N2E2_1632 0.22 +0.6 1,931,824 - Pf6N2E2_1632 0.22 +0.0 1,931,824 - Pf6N2E2_1632 0.22 -1.7 1,931,824 - Pf6N2E2_1632 0.22 +0.6 1,931,824 - Pf6N2E2_1632 0.22 -1.7 1,931,875 - Pf6N2E2_1632 0.28 -0.7 1,932,078 + Pf6N2E2_1632 0.50 -1.6 1,932,120 + Pf6N2E2_1632 0.55 +0.6 1,932,121 - Pf6N2E2_1632 0.55 -0.7 1,932,121 - Pf6N2E2_1632 0.55 -1.6 1,932,202 - Pf6N2E2_1632 0.64 +1.0 1,932,498 - -1.0
Or see this region's nucleotide sequence