Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1562 and Pf6N2E2_1563 are separated by 93 nucleotides Pf6N2E2_1563 and Pf6N2E2_1564 are separated by 242 nucleotides
Pf6N2E2_1562 - Threonine dehydratase (EC 4.3.1.19), at 1,823,631 to 1,824,587
_1562
Pf6N2E2_1563 - Transcriptional regulator, AraC family, at 1,824,681 to 1,825,496
_1563
Pf6N2E2_1564 - 3-dehydroquinate synthase (EC 4.2.3.4), at 1,825,739 to 1,827,613
_1564
Position (kb)
1824
1825
1826 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1823.782 kb on + strand, within Pf6N2E2_1562 at 1823.782 kb on + strand, within Pf6N2E2_1562 at 1823.782 kb on + strand, within Pf6N2E2_1562 at 1823.783 kb on - strand, within Pf6N2E2_1562 at 1823.783 kb on - strand, within Pf6N2E2_1562 at 1823.785 kb on + strand, within Pf6N2E2_1562 at 1823.786 kb on - strand, within Pf6N2E2_1562 at 1823.826 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.907 kb on - strand, within Pf6N2E2_1562 at 1823.907 kb on - strand, within Pf6N2E2_1562 at 1824.064 kb on + strand, within Pf6N2E2_1562 at 1824.064 kb on + strand, within Pf6N2E2_1562 at 1824.065 kb on - strand, within Pf6N2E2_1562 at 1824.065 kb on - strand, within Pf6N2E2_1562 at 1824.110 kb on - strand, within Pf6N2E2_1562 at 1824.276 kb on - strand, within Pf6N2E2_1562 at 1824.511 kb on + strand at 1824.538 kb on + strand at 1824.538 kb on + strand at 1824.539 kb on - strand at 1824.539 kb on - strand at 1824.539 kb on - strand at 1824.541 kb on - strand at 1824.541 kb on - strand at 1824.616 kb on - strand at 1824.678 kb on - strand at 1824.678 kb on - strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.799 kb on - strand, within Pf6N2E2_1563 at 1824.960 kb on + strand, within Pf6N2E2_1563 at 1824.960 kb on + strand, within Pf6N2E2_1563 at 1824.960 kb on + strand, within Pf6N2E2_1563 at 1825.160 kb on - strand, within Pf6N2E2_1563 at 1825.160 kb on - strand, within Pf6N2E2_1563 at 1825.282 kb on + strand, within Pf6N2E2_1563 at 1825.596 kb on - strand at 1825.661 kb on - strand at 1825.697 kb on + strand at 1825.697 kb on + strand at 1825.719 kb on - strand at 1825.719 kb on - strand at 1825.919 kb on + strand at 1826.298 kb on - strand, within Pf6N2E2_1564 at 1826.308 kb on - strand, within Pf6N2E2_1564 at 1826.364 kb on + strand, within Pf6N2E2_1564 at 1826.459 kb on + strand, within Pf6N2E2_1564 at 1826.460 kb on - strand, within Pf6N2E2_1564 at 1826.460 kb on - strand, within Pf6N2E2_1564 at 1826.490 kb on + strand at 1826.491 kb on - strand, within Pf6N2E2_1564 at 1826.491 kb on - strand, within Pf6N2E2_1564 at 1826.491 kb on - strand, within Pf6N2E2_1564 at 1826.491 kb on - strand, within Pf6N2E2_1564 at 1826.491 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,823,782 + Pf6N2E2_1562 0.16 +0.9 1,823,782 + Pf6N2E2_1562 0.16 -1.4 1,823,782 + Pf6N2E2_1562 0.16 +0.0 1,823,783 - Pf6N2E2_1562 0.16 +3.2 1,823,783 - Pf6N2E2_1562 0.16 +1.4 1,823,785 + Pf6N2E2_1562 0.16 +0.6 1,823,786 - Pf6N2E2_1562 0.16 +0.6 1,823,826 + Pf6N2E2_1562 0.20 -0.5 1,823,851 + Pf6N2E2_1562 0.23 -0.4 1,823,851 + Pf6N2E2_1562 0.23 +0.0 1,823,851 + Pf6N2E2_1562 0.23 +1.0 1,823,851 + Pf6N2E2_1562 0.23 +0.3 1,823,851 + Pf6N2E2_1562 0.23 -0.6 1,823,851 + Pf6N2E2_1562 0.23 +1.0 1,823,852 - Pf6N2E2_1562 0.23 -0.4 1,823,852 - Pf6N2E2_1562 0.23 +0.6 1,823,852 - Pf6N2E2_1562 0.23 +0.5 1,823,852 - Pf6N2E2_1562 0.23 -0.2 1,823,852 - Pf6N2E2_1562 0.23 +0.6 1,823,907 - Pf6N2E2_1562 0.29 -1.1 1,823,907 - Pf6N2E2_1562 0.29 -1.1 1,824,064 + Pf6N2E2_1562 0.45 -2.0 1,824,064 + Pf6N2E2_1562 0.45 -0.4 1,824,065 - Pf6N2E2_1562 0.45 -0.2 1,824,065 - Pf6N2E2_1562 0.45 -0.3 1,824,110 - Pf6N2E2_1562 0.50 +0.6 1,824,276 - Pf6N2E2_1562 0.67 +0.9 1,824,511 + +1.9 1,824,538 + +1.3 1,824,538 + -1.5 1,824,539 - -1.0 1,824,539 - +1.0 1,824,539 - -0.3 1,824,541 - -1.2 1,824,541 - +0.3 1,824,616 - +1.2 1,824,678 - +0.6 1,824,678 - +0.9 1,824,680 + -2.0 1,824,680 + +0.6 1,824,680 + +0.0 1,824,680 + +0.8 1,824,680 + +0.4 1,824,680 + -0.8 1,824,680 + -1.7 1,824,681 - -0.8 1,824,681 - +0.9 1,824,681 - +0.5 1,824,681 - +0.2 1,824,681 - -1.2 1,824,681 - -0.7 1,824,681 - -0.2 1,824,681 - +0.8 1,824,681 - +1.6 1,824,681 - +0.6 1,824,681 - +1.0 1,824,681 - +0.9 1,824,681 - +0.6 1,824,681 - -0.4 1,824,799 - Pf6N2E2_1563 0.14 +0.4 1,824,960 + Pf6N2E2_1563 0.34 -1.4 1,824,960 + Pf6N2E2_1563 0.34 -0.3 1,824,960 + Pf6N2E2_1563 0.34 -1.0 1,825,160 - Pf6N2E2_1563 0.59 -1.6 1,825,160 - Pf6N2E2_1563 0.59 -1.4 1,825,282 + Pf6N2E2_1563 0.74 -0.2 1,825,596 - -0.4 1,825,661 - -0.3 1,825,697 + -0.4 1,825,697 + -0.4 1,825,719 - +1.6 1,825,719 - +0.6 1,825,919 + +0.0 1,826,298 - Pf6N2E2_1564 0.30 +0.1 1,826,308 - Pf6N2E2_1564 0.30 +1.2 1,826,364 + Pf6N2E2_1564 0.33 +0.3 1,826,459 + Pf6N2E2_1564 0.38 +1.0 1,826,460 - Pf6N2E2_1564 0.38 -0.6 1,826,460 - Pf6N2E2_1564 0.38 +0.4 1,826,490 + +1.2 1,826,491 - Pf6N2E2_1564 0.40 +0.3 1,826,491 - Pf6N2E2_1564 0.40 -1.0 1,826,491 - Pf6N2E2_1564 0.40 -1.1 1,826,491 - Pf6N2E2_1564 0.40 +0.3 1,826,491 - +0.6
Or see this region's nucleotide sequence