Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1453 and Pf6N2E2_1454 are separated by 155 nucleotides Pf6N2E2_1454 and Pf6N2E2_1455 are separated by 147 nucleotides
Pf6N2E2_1453 - Xylose activator XylR (AraC family), at 1,700,452 to 1,701,639
_1453
Pf6N2E2_1454 - Xylose isomerase (EC 5.3.1.5), at 1,701,795 to 1,703,111
_1454
Pf6N2E2_1455 - Xylose ABC transporter, periplasmic xylose-binding protein XylF, at 1,703,259 to 1,704,260
_1455
Position (kb)
1701
1702
1703
1704 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1701.299 kb on + strand, within Pf6N2E2_1453 at 1701.299 kb on + strand, within Pf6N2E2_1453 at 1701.549 kb on - strand at 1701.645 kb on + strand at 1701.671 kb on - strand at 1701.695 kb on + strand at 1701.755 kb on - strand at 1701.777 kb on + strand at 1701.777 kb on + strand at 1701.778 kb on - strand at 1701.801 kb on + strand at 1701.802 kb on - strand at 1701.802 kb on - strand at 1701.802 kb on - strand at 1701.870 kb on + strand at 1701.870 kb on + strand at 1701.934 kb on - strand, within Pf6N2E2_1454 at 1701.941 kb on + strand, within Pf6N2E2_1454 at 1701.942 kb on - strand, within Pf6N2E2_1454 at 1701.942 kb on - strand, within Pf6N2E2_1454 at 1702.083 kb on + strand, within Pf6N2E2_1454 at 1702.344 kb on + strand, within Pf6N2E2_1454 at 1702.344 kb on + strand, within Pf6N2E2_1454 at 1702.344 kb on + strand, within Pf6N2E2_1454 at 1702.524 kb on + strand, within Pf6N2E2_1454 at 1702.525 kb on - strand, within Pf6N2E2_1454 at 1702.622 kb on + strand, within Pf6N2E2_1454 at 1702.623 kb on - strand, within Pf6N2E2_1454 at 1702.758 kb on + strand, within Pf6N2E2_1454 at 1702.759 kb on - strand, within Pf6N2E2_1454 at 1703.099 kb on + strand at 1703.099 kb on + strand at 1703.100 kb on - strand at 1703.100 kb on - strand at 1703.103 kb on + strand at 1703.103 kb on + strand at 1703.103 kb on + strand at 1703.104 kb on - strand at 1703.144 kb on + strand at 1703.145 kb on - strand at 1703.145 kb on - strand at 1703.145 kb on - strand at 1703.145 kb on - strand at 1703.189 kb on + strand at 1703.198 kb on - strand at 1703.198 kb on - strand at 1703.242 kb on + strand at 1703.243 kb on - strand at 1703.243 kb on - strand at 1703.397 kb on + strand, within Pf6N2E2_1455 at 1703.397 kb on + strand, within Pf6N2E2_1455 at 1703.398 kb on - strand, within Pf6N2E2_1455 at 1703.398 kb on - strand, within Pf6N2E2_1455 at 1703.398 kb on - strand, within Pf6N2E2_1455 at 1703.595 kb on + strand, within Pf6N2E2_1455 at 1703.596 kb on - strand, within Pf6N2E2_1455 at 1703.631 kb on + strand, within Pf6N2E2_1455 at 1703.631 kb on + strand, within Pf6N2E2_1455 at 1703.632 kb on - strand, within Pf6N2E2_1455 at 1703.706 kb on + strand, within Pf6N2E2_1455 at 1703.706 kb on + strand, within Pf6N2E2_1455 at 1704.052 kb on - strand, within Pf6N2E2_1455
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 1,701,299 + Pf6N2E2_1453 0.71 +0.5 1,701,299 + Pf6N2E2_1453 0.71 +0.5 1,701,549 - +1.1 1,701,645 + +0.7 1,701,671 - -1.1 1,701,695 + +0.5 1,701,755 - -0.5 1,701,777 + +0.5 1,701,777 + -1.8 1,701,778 - -1.6 1,701,801 + -0.5 1,701,802 - +0.8 1,701,802 - +0.5 1,701,802 - +1.5 1,701,870 + -0.0 1,701,870 + -1.0 1,701,934 - Pf6N2E2_1454 0.11 -0.0 1,701,941 + Pf6N2E2_1454 0.11 -0.5 1,701,942 - Pf6N2E2_1454 0.11 +1.5 1,701,942 - Pf6N2E2_1454 0.11 -1.1 1,702,083 + Pf6N2E2_1454 0.22 -1.8 1,702,344 + Pf6N2E2_1454 0.42 -1.0 1,702,344 + Pf6N2E2_1454 0.42 +0.4 1,702,344 + Pf6N2E2_1454 0.42 +0.9 1,702,524 + Pf6N2E2_1454 0.55 -1.0 1,702,525 - Pf6N2E2_1454 0.55 -0.5 1,702,622 + Pf6N2E2_1454 0.63 -0.9 1,702,623 - Pf6N2E2_1454 0.63 -1.3 1,702,758 + Pf6N2E2_1454 0.73 +1.5 1,702,759 - Pf6N2E2_1454 0.73 +1.0 1,703,099 + +0.3 1,703,099 + -0.7 1,703,100 - -1.0 1,703,100 - +0.5 1,703,103 + -0.5 1,703,103 + +1.0 1,703,103 + +0.8 1,703,104 - -0.6 1,703,144 + -0.1 1,703,145 - +0.9 1,703,145 - -1.1 1,703,145 - -0.1 1,703,145 - -0.5 1,703,189 + +1.1 1,703,198 - +0.5 1,703,198 - +0.2 1,703,242 + -0.6 1,703,243 - -0.3 1,703,243 - -1.7 1,703,397 + Pf6N2E2_1455 0.14 +1.9 1,703,397 + Pf6N2E2_1455 0.14 -1.8 1,703,398 - Pf6N2E2_1455 0.14 +0.5 1,703,398 - Pf6N2E2_1455 0.14 +1.9 1,703,398 - Pf6N2E2_1455 0.14 +0.4 1,703,595 + Pf6N2E2_1455 0.34 +2.5 1,703,596 - Pf6N2E2_1455 0.34 -0.3 1,703,631 + Pf6N2E2_1455 0.37 -0.5 1,703,631 + Pf6N2E2_1455 0.37 -0.1 1,703,632 - Pf6N2E2_1455 0.37 -0.5 1,703,706 + Pf6N2E2_1455 0.45 -0.2 1,703,706 + Pf6N2E2_1455 0.45 -2.3 1,704,052 - Pf6N2E2_1455 0.79 -0.5
Or see this region's nucleotide sequence