Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1333

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1332 and Pf6N2E2_1333 are separated by 69 nucleotidesPf6N2E2_1333 and Pf6N2E2_1334 are separated by 111 nucleotides Pf6N2E2_1332 - Protein involved in meta-pathway of phenol degradation, at 1,567,754 to 1,568,686 _1332 Pf6N2E2_1333 - Ethanolamine operon regulatory protein, at 1,568,756 to 1,569,748 _1333 Pf6N2E2_1334 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,569,860 to 1,570,867 _1334 Position (kb) 1568 1569 1570Strain fitness (log2 ratio) -2 -1 0 1 2at 1567.772 kb on - strandat 1567.786 kb on + strandat 1567.824 kb on + strandat 1567.824 kb on + strandat 1567.824 kb on + strandat 1567.825 kb on - strandat 1567.863 kb on + strand, within Pf6N2E2_1332at 1567.863 kb on + strand, within Pf6N2E2_1332at 1567.863 kb on + strand, within Pf6N2E2_1332at 1567.863 kb on + strand, within Pf6N2E2_1332at 1567.864 kb on - strand, within Pf6N2E2_1332at 1567.864 kb on - strand, within Pf6N2E2_1332at 1567.948 kb on - strand, within Pf6N2E2_1332at 1567.950 kb on + strand, within Pf6N2E2_1332at 1567.950 kb on + strand, within Pf6N2E2_1332at 1567.951 kb on - strand, within Pf6N2E2_1332at 1568.031 kb on + strand, within Pf6N2E2_1332at 1568.031 kb on + strand, within Pf6N2E2_1332at 1568.032 kb on - strand, within Pf6N2E2_1332at 1568.106 kb on + strand, within Pf6N2E2_1332at 1568.107 kb on - strand, within Pf6N2E2_1332at 1568.107 kb on - strand, within Pf6N2E2_1332at 1568.373 kb on + strand, within Pf6N2E2_1332at 1568.373 kb on + strand, within Pf6N2E2_1332at 1568.373 kb on + strand, within Pf6N2E2_1332at 1568.373 kb on + strand, within Pf6N2E2_1332at 1568.373 kb on + strand, within Pf6N2E2_1332at 1568.374 kb on - strand, within Pf6N2E2_1332at 1568.412 kb on + strand, within Pf6N2E2_1332at 1568.412 kb on + strand, within Pf6N2E2_1332at 1568.413 kb on - strand, within Pf6N2E2_1332at 1568.413 kb on - strand, within Pf6N2E2_1332at 1568.413 kb on - strand, within Pf6N2E2_1332at 1568.497 kb on - strand, within Pf6N2E2_1332at 1568.497 kb on - strand, within Pf6N2E2_1332at 1568.506 kb on + strand, within Pf6N2E2_1332at 1568.507 kb on - strand, within Pf6N2E2_1332at 1568.507 kb on - strand, within Pf6N2E2_1332at 1568.507 kb on - strand, within Pf6N2E2_1332at 1568.565 kb on + strand, within Pf6N2E2_1332at 1568.565 kb on + strand, within Pf6N2E2_1332at 1568.566 kb on - strand, within Pf6N2E2_1332at 1568.651 kb on - strandat 1568.651 kb on - strandat 1568.937 kb on + strand, within Pf6N2E2_1333at 1568.937 kb on + strand, within Pf6N2E2_1333at 1569.057 kb on + strand, within Pf6N2E2_1333at 1569.057 kb on + strand, within Pf6N2E2_1333at 1569.058 kb on - strand, within Pf6N2E2_1333at 1569.137 kb on + strand, within Pf6N2E2_1333at 1569.138 kb on - strand, within Pf6N2E2_1333at 1569.403 kb on + strand, within Pf6N2E2_1333at 1569.422 kb on + strand, within Pf6N2E2_1333at 1569.422 kb on + strand, within Pf6N2E2_1333at 1569.622 kb on - strand, within Pf6N2E2_1333at 1569.897 kb on + strandat 1569.957 kb on + strandat 1569.957 kb on + strandat 1569.957 kb on + strandat 1569.957 kb on + strandat 1569.958 kb on - strandat 1569.958 kb on - strandat 1569.963 kb on + strand, within Pf6N2E2_1334at 1569.963 kb on + strand, within Pf6N2E2_1334at 1569.964 kb on - strand, within Pf6N2E2_1334at 1569.964 kb on - strand, within Pf6N2E2_1334at 1569.964 kb on - strand, within Pf6N2E2_1334at 1570.059 kb on + strand, within Pf6N2E2_1334at 1570.060 kb on - strand, within Pf6N2E2_1334at 1570.060 kb on - strand, within Pf6N2E2_1334at 1570.123 kb on - strand, within Pf6N2E2_1334at 1570.123 kb on - strand, within Pf6N2E2_1334at 1570.123 kb on - strand, within Pf6N2E2_1334at 1570.134 kb on + strand, within Pf6N2E2_1334at 1570.173 kb on + strand, within Pf6N2E2_1334at 1570.174 kb on - strand, within Pf6N2E2_1334at 1570.212 kb on + strand, within Pf6N2E2_1334at 1570.212 kb on + strand, within Pf6N2E2_1334at 1570.213 kb on - strand, within Pf6N2E2_1334at 1570.213 kb on - strand, within Pf6N2E2_1334at 1570.509 kb on + strand, within Pf6N2E2_1334

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,567,772 - +0.1
1,567,786 + -0.5
1,567,824 + -1.1
1,567,824 + +1.0
1,567,824 + -0.1
1,567,825 - +0.2
1,567,863 + Pf6N2E2_1332 0.12 -0.2
1,567,863 + Pf6N2E2_1332 0.12 +1.1
1,567,863 + Pf6N2E2_1332 0.12 +0.5
1,567,863 + Pf6N2E2_1332 0.12 -1.8
1,567,864 - Pf6N2E2_1332 0.12 +1.5
1,567,864 - Pf6N2E2_1332 0.12 +1.1
1,567,948 - Pf6N2E2_1332 0.21 +0.5
1,567,950 + Pf6N2E2_1332 0.21 +0.8
1,567,950 + Pf6N2E2_1332 0.21 +0.9
1,567,951 - Pf6N2E2_1332 0.21 -0.1
1,568,031 + Pf6N2E2_1332 0.30 -0.1
1,568,031 + Pf6N2E2_1332 0.30 -0.1
1,568,032 - Pf6N2E2_1332 0.30 +0.5
1,568,106 + Pf6N2E2_1332 0.38 +0.5
1,568,107 - Pf6N2E2_1332 0.38 +0.8
1,568,107 - Pf6N2E2_1332 0.38 -0.5
1,568,373 + Pf6N2E2_1332 0.66 +0.1
1,568,373 + Pf6N2E2_1332 0.66 +1.0
1,568,373 + Pf6N2E2_1332 0.66 -1.3
1,568,373 + Pf6N2E2_1332 0.66 +1.1
1,568,373 + Pf6N2E2_1332 0.66 +1.5
1,568,374 - Pf6N2E2_1332 0.66 +1.8
1,568,412 + Pf6N2E2_1332 0.71 -0.5
1,568,412 + Pf6N2E2_1332 0.71 +0.5
1,568,413 - Pf6N2E2_1332 0.71 +1.1
1,568,413 - Pf6N2E2_1332 0.71 -1.1
1,568,413 - Pf6N2E2_1332 0.71 -0.5
1,568,497 - Pf6N2E2_1332 0.80 -0.5
1,568,497 - Pf6N2E2_1332 0.80 -0.6
1,568,506 + Pf6N2E2_1332 0.81 +0.5
1,568,507 - Pf6N2E2_1332 0.81 -0.5
1,568,507 - Pf6N2E2_1332 0.81 +0.3
1,568,507 - Pf6N2E2_1332 0.81 -0.1
1,568,565 + Pf6N2E2_1332 0.87 +0.0
1,568,565 + Pf6N2E2_1332 0.87 -2.1
1,568,566 - Pf6N2E2_1332 0.87 -0.8
1,568,651 - +0.5
1,568,651 - -1.1
1,568,937 + Pf6N2E2_1333 0.18 -0.7
1,568,937 + Pf6N2E2_1333 0.18 -0.9
1,569,057 + Pf6N2E2_1333 0.30 +0.5
1,569,057 + Pf6N2E2_1333 0.30 -0.2
1,569,058 - Pf6N2E2_1333 0.30 +0.0
1,569,137 + Pf6N2E2_1333 0.38 +0.4
1,569,138 - Pf6N2E2_1333 0.38 -0.5
1,569,403 + Pf6N2E2_1333 0.65 -0.9
1,569,422 + Pf6N2E2_1333 0.67 -0.1
1,569,422 + Pf6N2E2_1333 0.67 -0.4
1,569,622 - Pf6N2E2_1333 0.87 -0.5
1,569,897 + -0.8
1,569,957 + +0.8
1,569,957 + +0.5
1,569,957 + +0.7
1,569,957 + -0.1
1,569,958 - -0.3
1,569,958 - +0.2
1,569,963 + Pf6N2E2_1334 0.10 -0.1
1,569,963 + Pf6N2E2_1334 0.10 -0.2
1,569,964 - Pf6N2E2_1334 0.10 +0.2
1,569,964 - Pf6N2E2_1334 0.10 -1.2
1,569,964 - Pf6N2E2_1334 0.10 +0.2
1,570,059 + Pf6N2E2_1334 0.20 +0.2
1,570,060 - Pf6N2E2_1334 0.20 -0.3
1,570,060 - Pf6N2E2_1334 0.20 -0.5
1,570,123 - Pf6N2E2_1334 0.26 -0.9
1,570,123 - Pf6N2E2_1334 0.26 -0.7
1,570,123 - Pf6N2E2_1334 0.26 -0.0
1,570,134 + Pf6N2E2_1334 0.27 +0.3
1,570,173 + Pf6N2E2_1334 0.31 -2.7
1,570,174 - Pf6N2E2_1334 0.31 +2.1
1,570,212 + Pf6N2E2_1334 0.35 +0.8
1,570,212 + Pf6N2E2_1334 0.35 +1.5
1,570,213 - Pf6N2E2_1334 0.35 -0.1
1,570,213 - Pf6N2E2_1334 0.35 -1.5
1,570,509 + Pf6N2E2_1334 0.64 +0.5

Or see this region's nucleotide sequence