Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_127 and Pf6N2E2_128 overlap by 1 nucleotides Pf6N2E2_128 and Pf6N2E2_129 overlap by 11 nucleotides
Pf6N2E2_127 - AttE component of AttEFGH ABC transport system, at 176,445 to 177,113
_127
Pf6N2E2_128 - AttF component of AttEFGH ABC transport system / AttG component of AttEFGH ABC transport system, at 177,113 to 179,581
_128
Pf6N2E2_129 - AttH component of AttEFGH ABC transport system, at 179,571 to 180,644
_129
Position (kb) 177
178
179
180 Strain fitness (log2 ratio) -2
-1
0
1
2 at 176.336 kb on + strand at 176.336 kb on + strand at 176.336 kb on + strand at 176.337 kb on - strand at 176.337 kb on - strand at 176.337 kb on - strand at 176.337 kb on - strand at 176.359 kb on - strand at 176.359 kb on - strand at 176.359 kb on - strand at 176.359 kb on - strand at 176.376 kb on + strand at 176.377 kb on - strand at 176.377 kb on - strand at 176.812 kb on + strand, within Pf6N2E2_127 at 176.986 kb on + strand, within Pf6N2E2_127 at 177.074 kb on - strand at 177.074 kb on - strand at 177.257 kb on + strand at 177.258 kb on - strand at 177.258 kb on - strand at 177.518 kb on - strand, within Pf6N2E2_128 at 177.684 kb on + strand, within Pf6N2E2_128 at 178.616 kb on + strand, within Pf6N2E2_128 at 178.616 kb on + strand, within Pf6N2E2_128 at 178.617 kb on - strand, within Pf6N2E2_128 at 178.617 kb on - strand, within Pf6N2E2_128 at 178.617 kb on - strand, within Pf6N2E2_128 at 178.946 kb on + strand, within Pf6N2E2_128 at 178.946 kb on + strand, within Pf6N2E2_128 at 178.946 kb on + strand, within Pf6N2E2_128 at 178.946 kb on + strand, within Pf6N2E2_128 at 178.947 kb on - strand, within Pf6N2E2_128 at 179.070 kb on + strand, within Pf6N2E2_128 at 179.531 kb on + strand at 179.532 kb on - strand at 179.579 kb on + strand at 179.579 kb on + strand at 179.580 kb on - strand at 179.704 kb on + strand, within Pf6N2E2_129 at 179.760 kb on + strand, within Pf6N2E2_129 at 179.760 kb on + strand, within Pf6N2E2_129 at 179.761 kb on - strand, within Pf6N2E2_129 at 180.075 kb on + strand, within Pf6N2E2_129 at 180.076 kb on - strand, within Pf6N2E2_129 at 180.076 kb on - strand, within Pf6N2E2_129 at 180.162 kb on + strand, within Pf6N2E2_129 at 180.162 kb on + strand, within Pf6N2E2_129 at 180.162 kb on + strand, within Pf6N2E2_129 at 180.163 kb on - strand, within Pf6N2E2_129 at 180.163 kb on - strand, within Pf6N2E2_129 at 180.163 kb on - strand, within Pf6N2E2_129 at 180.163 kb on - strand, within Pf6N2E2_129 at 180.165 kb on + strand, within Pf6N2E2_129 at 180.166 kb on - strand, within Pf6N2E2_129 at 180.168 kb on + strand, within Pf6N2E2_129 at 180.168 kb on + strand, within Pf6N2E2_129 at 180.168 kb on + strand, within Pf6N2E2_129 at 180.169 kb on - strand, within Pf6N2E2_129 at 180.169 kb on - strand, within Pf6N2E2_129 at 180.372 kb on + strand, within Pf6N2E2_129
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 176,336 + +0.8 176,336 + -0.1 176,336 + -0.2 176,337 - -0.1 176,337 - +1.8 176,337 - +1.0 176,337 - +0.1 176,359 - -0.0 176,359 - -0.8 176,359 - +0.4 176,359 - +0.1 176,376 + -0.4 176,377 - +0.2 176,377 - -0.2 176,812 + Pf6N2E2_127 0.55 -0.6 176,986 + Pf6N2E2_127 0.81 +0.4 177,074 - -0.6 177,074 - -1.0 177,257 + +0.5 177,258 - -1.2 177,258 - +1.0 177,518 - Pf6N2E2_128 0.16 -0.8 177,684 + Pf6N2E2_128 0.23 -0.9 178,616 + Pf6N2E2_128 0.61 -1.1 178,616 + Pf6N2E2_128 0.61 -0.2 178,617 - Pf6N2E2_128 0.61 -1.1 178,617 - Pf6N2E2_128 0.61 -0.2 178,617 - Pf6N2E2_128 0.61 -0.2 178,946 + Pf6N2E2_128 0.74 -1.8 178,946 + Pf6N2E2_128 0.74 -1.0 178,946 + Pf6N2E2_128 0.74 +0.8 178,946 + Pf6N2E2_128 0.74 -0.1 178,947 - Pf6N2E2_128 0.74 -0.1 179,070 + Pf6N2E2_128 0.79 -1.1 179,531 + +0.8 179,532 - +0.5 179,579 + -0.0 179,579 + -0.0 179,580 - +1.4 179,704 + Pf6N2E2_129 0.12 -0.5 179,760 + Pf6N2E2_129 0.18 +0.2 179,760 + Pf6N2E2_129 0.18 +0.0 179,761 - Pf6N2E2_129 0.18 -0.8 180,075 + Pf6N2E2_129 0.47 +0.4 180,076 - Pf6N2E2_129 0.47 +0.4 180,076 - Pf6N2E2_129 0.47 -0.2 180,162 + Pf6N2E2_129 0.55 +0.8 180,162 + Pf6N2E2_129 0.55 -0.3 180,162 + Pf6N2E2_129 0.55 -0.0 180,163 - Pf6N2E2_129 0.55 -0.5 180,163 - Pf6N2E2_129 0.55 -0.2 180,163 - Pf6N2E2_129 0.55 +0.3 180,163 - Pf6N2E2_129 0.55 -2.4 180,165 + Pf6N2E2_129 0.55 +0.2 180,166 - Pf6N2E2_129 0.55 -0.1 180,168 + Pf6N2E2_129 0.56 +0.8 180,168 + Pf6N2E2_129 0.56 -0.0 180,168 + Pf6N2E2_129 0.56 +0.1 180,169 - Pf6N2E2_129 0.56 +0.3 180,169 - Pf6N2E2_129 0.56 -1.4 180,372 + Pf6N2E2_129 0.75 +1.5
Or see this region's nucleotide sequence