Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS09935 and HSERO_RS09940 are separated by 123 nucleotides HSERO_RS09940 and HSERO_RS09945 are separated by 78 nucleotides HSERO_RS09945 and HSERO_RS09950 are separated by 64 nucleotides
HSERO_RS09935: HSERO_RS09935 - UDP-phosphate glucose phosphotransferase, at 2,245,281 to 2,246,666
_RS09935
HSERO_RS09940: HSERO_RS09940 - peptidylprolyl isomerase, at 2,246,790 to 2,247,638
_RS09940
HSERO_RS09945: HSERO_RS09945 - sugar transporter, at 2,247,717 to 2,248,511
_RS09945
HSERO_RS09950: HSERO_RS09950 - chain-length determining protein, at 2,248,576 to 2,249,976
_RS09950
Position (kb)
2246
2247
2248 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7
8
9 at 2246.101 kb on + strand, within HSERO_RS09935 at 2246.119 kb on + strand, within HSERO_RS09935 at 2246.473 kb on + strand, within HSERO_RS09935 at 2246.610 kb on - strand at 2246.696 kb on - strand at 2246.852 kb on - strand at 2246.852 kb on - strand at 2246.852 kb on - strand at 2246.862 kb on - strand at 2247.082 kb on - strand, within HSERO_RS09940 at 2247.144 kb on - strand, within HSERO_RS09940 at 2247.144 kb on - strand at 2247.144 kb on - strand, within HSERO_RS09940 at 2247.484 kb on - strand at 2247.896 kb on + strand, within HSERO_RS09945 at 2247.938 kb on + strand, within HSERO_RS09945 at 2248.267 kb on - strand, within HSERO_RS09945 at 2248.353 kb on + strand, within HSERO_RS09945 at 2248.382 kb on + strand, within HSERO_RS09945 at 2248.402 kb on - strand, within HSERO_RS09945 at 2248.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI remove 2,246,101 + HSERO_RS09935 0.59 -0.3 2,246,119 + HSERO_RS09935 0.60 -0.1 2,246,473 + HSERO_RS09935 0.86 +0.9 2,246,610 - -0.6 2,246,696 - +0.4 2,246,852 - +0.1 2,246,852 - -1.5 2,246,852 - -0.8 2,246,862 - +0.5 2,247,082 - HSERO_RS09940 0.34 +0.0 2,247,144 - HSERO_RS09940 0.42 -0.6 2,247,144 - +0.7 2,247,144 - HSERO_RS09940 0.42 +9.0 2,247,484 - +2.5 2,247,896 + HSERO_RS09945 0.23 +0.5 2,247,938 + HSERO_RS09945 0.28 +0.3 2,248,267 - HSERO_RS09945 0.69 -1.6 2,248,353 + HSERO_RS09945 0.80 -0.9 2,248,382 + HSERO_RS09945 0.84 +0.2 2,248,402 - HSERO_RS09945 0.86 -0.7 2,248,561 - +0.2
Or see this region's nucleotide sequence