Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3190

Experiment: Cobalt chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3189 and Pf6N2E2_3190 are separated by 50 nucleotidesPf6N2E2_3190 and Pf6N2E2_3191 are separated by 304 nucleotides Pf6N2E2_3189 - Cysteine desulfurase (EC 2.8.1.7), IscS subfamily, at 3,682,826 to 3,684,040 _3189 Pf6N2E2_3190 - Iron-sulfur cluster regulator IscR, at 3,684,091 to 3,684,543 _3190 Pf6N2E2_3191 - Serine acetyltransferase (EC 2.3.1.30), at 3,684,848 to 3,685,624 _3191 Position (kb) 3684 3685Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 3684.139 kb on - strand, within Pf6N2E2_3190at 3684.139 kb on - strand, within Pf6N2E2_3190at 3684.194 kb on - strand, within Pf6N2E2_3190at 3684.277 kb on - strand, within Pf6N2E2_3190at 3684.277 kb on - strand, within Pf6N2E2_3190at 3684.462 kb on - strand, within Pf6N2E2_3190at 3684.571 kb on - strandat 3684.591 kb on - strandat 3684.697 kb on + strandat 3684.780 kb on + strandat 3684.780 kb on + strandat 3684.781 kb on - strandat 3684.781 kb on - strandat 3684.795 kb on + strandat 3684.849 kb on + strandat 3684.927 kb on + strand, within Pf6N2E2_3191at 3684.928 kb on - strand, within Pf6N2E2_3191at 3684.928 kb on - strand, within Pf6N2E2_3191at 3684.931 kb on + strand, within Pf6N2E2_3191at 3684.931 kb on + strand, within Pf6N2E2_3191at 3684.932 kb on - strand, within Pf6N2E2_3191at 3684.932 kb on - strand, within Pf6N2E2_3191at 3684.932 kb on - strand, within Pf6N2E2_3191at 3684.932 kb on - strand, within Pf6N2E2_3191at 3685.054 kb on - strand, within Pf6N2E2_3191

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM
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3,684,139 - Pf6N2E2_3190 0.11 -3.3
3,684,139 - Pf6N2E2_3190 0.11 -2.4
3,684,194 - Pf6N2E2_3190 0.23 -0.4
3,684,277 - Pf6N2E2_3190 0.41 +2.7
3,684,277 - Pf6N2E2_3190 0.41 +3.3
3,684,462 - Pf6N2E2_3190 0.82 -2.2
3,684,571 - -0.7
3,684,591 - -2.8
3,684,697 + -1.7
3,684,780 + -2.2
3,684,780 + -2.0
3,684,781 - -2.6
3,684,781 - -3.3
3,684,795 + -1.0
3,684,849 + -1.7
3,684,927 + Pf6N2E2_3191 0.10 -1.7
3,684,928 - Pf6N2E2_3191 0.10 -1.5
3,684,928 - Pf6N2E2_3191 0.10 -2.0
3,684,931 + Pf6N2E2_3191 0.11 +0.3
3,684,931 + Pf6N2E2_3191 0.11 -2.9
3,684,932 - Pf6N2E2_3191 0.11 -1.7
3,684,932 - Pf6N2E2_3191 0.11 -1.2
3,684,932 - Pf6N2E2_3191 0.11 -2.3
3,684,932 - Pf6N2E2_3191 0.11 -1.5
3,685,054 - Pf6N2E2_3191 0.27 +4.0

Or see this region's nucleotide sequence