Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2840 and Pf6N2E2_2841 are separated by 404 nucleotides Pf6N2E2_2841 and Pf6N2E2_2842 are separated by 169 nucleotides Pf6N2E2_2842 and Pf6N2E2_2843 are separated by 144 nucleotides Pf6N2E2_2843 and Pf6N2E2_2844 are separated by 94 nucleotides
Pf6N2E2_2840 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,330,289 to 3,330,489
_2840
Pf6N2E2_2841 - Low-affinity inorganic phosphate transporter, at 3,330,894 to 3,332,330
_2841
Pf6N2E2_2842 - hypothetical protein, at 3,332,500 to 3,332,616
_2842
Pf6N2E2_2843 - FIG00959308: hypothetical protein, at 3,332,761 to 3,332,922
_2843
Pf6N2E2_2844 - Aspartate-semialdehyde dehydrogenase (EC 1.2.1.11), at 3,333,017 to 3,333,358
_2844
Position (kb)
3330
3331
3332
3333 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3329.957 kb on - strand at 3329.998 kb on + strand at 3329.998 kb on + strand at 3329.998 kb on + strand at 3329.998 kb on + strand at 3329.999 kb on - strand at 3329.999 kb on - strand at 3329.999 kb on - strand at 3330.032 kb on + strand at 3330.052 kb on + strand at 3330.053 kb on - strand at 3330.068 kb on + strand at 3330.069 kb on - strand at 3330.069 kb on - strand at 3330.070 kb on + strand at 3330.070 kb on + strand at 3330.071 kb on - strand at 3330.127 kb on + strand at 3330.127 kb on + strand at 3330.127 kb on + strand at 3330.127 kb on + strand at 3330.131 kb on - strand at 3330.153 kb on + strand at 3330.154 kb on - strand at 3330.170 kb on - strand at 3330.198 kb on - strand at 3330.198 kb on - strand at 3330.198 kb on - strand at 3330.239 kb on + strand at 3330.239 kb on + strand at 3330.240 kb on - strand at 3330.240 kb on - strand at 3330.274 kb on + strand at 3330.274 kb on + strand at 3330.274 kb on + strand at 3330.275 kb on - strand at 3330.275 kb on - strand at 3330.278 kb on + strand at 3330.279 kb on - strand at 3330.279 kb on - strand at 3330.282 kb on + strand at 3330.282 kb on + strand at 3330.282 kb on + strand at 3330.282 kb on + strand at 3330.338 kb on - strand, within Pf6N2E2_2840 at 3330.338 kb on - strand, within Pf6N2E2_2840 at 3330.338 kb on - strand, within Pf6N2E2_2840 at 3330.370 kb on + strand, within Pf6N2E2_2840 at 3330.370 kb on + strand, within Pf6N2E2_2840 at 3330.370 kb on + strand, within Pf6N2E2_2840 at 3330.370 kb on + strand, within Pf6N2E2_2840 at 3330.371 kb on - strand, within Pf6N2E2_2840 at 3330.371 kb on - strand, within Pf6N2E2_2840 at 3330.371 kb on - strand, within Pf6N2E2_2840 at 3330.371 kb on - strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.396 kb on + strand, within Pf6N2E2_2840 at 3330.397 kb on - strand, within Pf6N2E2_2840 at 3330.409 kb on - strand, within Pf6N2E2_2840 at 3330.631 kb on - strand at 3330.657 kb on + strand at 3330.657 kb on + strand at 3330.749 kb on + strand at 3332.380 kb on + strand at 3332.386 kb on + strand at 3332.387 kb on - strand at 3332.501 kb on + strand at 3332.540 kb on - strand, within Pf6N2E2_2842 at 3332.560 kb on + strand, within Pf6N2E2_2842 at 3332.731 kb on - strand at 3332.907 kb on + strand at 3332.997 kb on + strand at 3332.998 kb on - strand at 3333.282 kb on - strand, within Pf6N2E2_2844 at 3333.282 kb on - strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.322 kb on + strand, within Pf6N2E2_2844 at 3333.323 kb on - strand, within Pf6N2E2_2844 at 3333.326 kb on + strand at 3333.327 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 3,329,957 - -0.0 3,329,998 + +0.8 3,329,998 + +1.5 3,329,998 + +1.0 3,329,998 + +1.3 3,329,999 - -1.0 3,329,999 - -1.7 3,329,999 - +0.8 3,330,032 + +1.6 3,330,052 + -0.3 3,330,053 - -1.3 3,330,068 + +0.7 3,330,069 - +1.3 3,330,069 - -2.5 3,330,070 + +0.3 3,330,070 + +0.3 3,330,071 - +0.6 3,330,127 + +0.3 3,330,127 + +0.7 3,330,127 + +3.0 3,330,127 + +2.3 3,330,131 - -2.4 3,330,153 + +0.7 3,330,154 - -1.0 3,330,170 - -2.2 3,330,198 - -0.2 3,330,198 - -0.7 3,330,198 - +0.8 3,330,239 + -0.1 3,330,239 + +1.3 3,330,240 - +0.7 3,330,240 - +0.3 3,330,274 + -2.0 3,330,274 + +1.5 3,330,274 + -1.3 3,330,275 - -1.3 3,330,275 - +0.4 3,330,278 + +1.8 3,330,279 - -0.6 3,330,279 - -1.7 3,330,282 + +0.6 3,330,282 + +1.9 3,330,282 + +0.3 3,330,282 + -0.7 3,330,338 - Pf6N2E2_2840 0.24 +2.6 3,330,338 - Pf6N2E2_2840 0.24 +0.4 3,330,338 - Pf6N2E2_2840 0.24 +1.3 3,330,370 + Pf6N2E2_2840 0.40 -2.2 3,330,370 + Pf6N2E2_2840 0.40 -0.8 3,330,370 + Pf6N2E2_2840 0.40 -0.7 3,330,370 + Pf6N2E2_2840 0.40 +1.0 3,330,371 - Pf6N2E2_2840 0.41 -1.0 3,330,371 - Pf6N2E2_2840 0.41 -1.0 3,330,371 - Pf6N2E2_2840 0.41 +2.3 3,330,371 - Pf6N2E2_2840 0.41 +0.3 3,330,396 + Pf6N2E2_2840 0.53 +2.3 3,330,396 + Pf6N2E2_2840 0.53 -1.7 3,330,396 + Pf6N2E2_2840 0.53 -0.8 3,330,396 + Pf6N2E2_2840 0.53 -0.5 3,330,396 + Pf6N2E2_2840 0.53 -2.0 3,330,396 + Pf6N2E2_2840 0.53 +0.3 3,330,397 - Pf6N2E2_2840 0.54 -0.9 3,330,409 - Pf6N2E2_2840 0.60 +0.5 3,330,631 - +0.3 3,330,657 + -0.7 3,330,657 + +0.3 3,330,749 + -0.0 3,332,380 + +0.8 3,332,386 + -2.5 3,332,387 - -0.8 3,332,501 + -1.9 3,332,540 - Pf6N2E2_2842 0.34 -0.8 3,332,560 + Pf6N2E2_2842 0.51 +0.5 3,332,731 - +0.1 3,332,907 + -0.4 3,332,997 + +1.0 3,332,998 - -0.3 3,333,282 - Pf6N2E2_2844 0.77 +0.3 3,333,282 - Pf6N2E2_2844 0.77 -0.0 3,333,322 + Pf6N2E2_2844 0.89 -0.7 3,333,322 + Pf6N2E2_2844 0.89 +0.3 3,333,322 + Pf6N2E2_2844 0.89 -0.6 3,333,322 + Pf6N2E2_2844 0.89 +1.0 3,333,322 + Pf6N2E2_2844 0.89 -1.0 3,333,322 + Pf6N2E2_2844 0.89 +0.7 3,333,322 + Pf6N2E2_2844 0.89 -0.0 3,333,322 + Pf6N2E2_2844 0.89 -0.8 3,333,323 - Pf6N2E2_2844 0.89 -1.3 3,333,326 + +1.3 3,333,327 - -2.6
Or see this region's nucleotide sequence