Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2555

Experiment: Cobalt chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2553 and Pf6N2E2_2554 overlap by 4 nucleotidesPf6N2E2_2554 and Pf6N2E2_2555 overlap by 4 nucleotidesPf6N2E2_2555 and Pf6N2E2_2556 are separated by 3 nucleotidesPf6N2E2_2556 and Pf6N2E2_2557 overlap by 4 nucleotidesPf6N2E2_2557 and Pf6N2E2_2558 overlap by 4 nucleotides Pf6N2E2_2552 - Cytochrome c heme lyase subunit CcmH, at 3,012,666 to 3,013,868 _2552 Pf6N2E2_2553 - Cytochrome c heme lyase subunit CcmL, at 3,013,861 to 3,014,334 _2553 Pf6N2E2_2554 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 3,014,331 to 3,014,867 _2554 Pf6N2E2_2555 - Cytochrome c heme lyase subunit CcmF, at 3,014,864 to 3,016,852 _2555 Pf6N2E2_2556 - Cytochrome c-type biogenesis protein CcmE, heme chaperone, at 3,016,856 to 3,017,311 _2556 Pf6N2E2_2557 - Cytochrome c-type biogenesis protein CcmD, interacts with CcmCE, at 3,017,308 to 3,017,484 _2557 Pf6N2E2_2558 - Cytochrome c-type biogenesis protein CcmC, putative heme lyase for CcmE, at 3,017,481 to 3,018,236 _2558 Position (kb) 3014 3015 3016 3017Strain fitness (log2 ratio) -1 0 1 2 3at 3014.363 kb on - strandat 3016.835 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM
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3,014,363 - +2.3
3,016,835 - +3.0

Or see this region's nucleotide sequence