Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1955 and Pf6N2E2_1956 are separated by 49 nucleotides Pf6N2E2_1956 and Pf6N2E2_1957 are separated by 3 nucleotides
Pf6N2E2_1955 - daunorubicin C-13 ketoreductase XF1741, at 2,319,960 to 2,320,559
_1955
Pf6N2E2_1956 - Outer membrane protein romA, at 2,320,609 to 2,321,745
_1956
Pf6N2E2_1957 - putative inner membrane protein, at 2,321,749 to 2,323,065
_1957
Position (kb)
2320
2321
2322 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2319.678 kb on - strand at 2319.678 kb on - strand at 2319.818 kb on + strand at 2319.818 kb on + strand at 2319.818 kb on + strand at 2319.819 kb on - strand at 2320.099 kb on + strand, within Pf6N2E2_1955 at 2320.099 kb on + strand, within Pf6N2E2_1955 at 2320.100 kb on - strand, within Pf6N2E2_1955 at 2320.122 kb on + strand, within Pf6N2E2_1955 at 2320.122 kb on + strand, within Pf6N2E2_1955 at 2320.123 kb on - strand, within Pf6N2E2_1955 at 2320.123 kb on - strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.164 kb on + strand, within Pf6N2E2_1955 at 2320.164 kb on + strand, within Pf6N2E2_1955 at 2320.165 kb on - strand, within Pf6N2E2_1955 at 2320.165 kb on - strand, within Pf6N2E2_1955 at 2320.165 kb on - strand, within Pf6N2E2_1955 at 2320.185 kb on - strand, within Pf6N2E2_1955 at 2320.246 kb on - strand, within Pf6N2E2_1955 at 2320.316 kb on + strand, within Pf6N2E2_1955 at 2320.317 kb on - strand, within Pf6N2E2_1955 at 2320.573 kb on - strand at 2320.576 kb on + strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.590 kb on - strand at 2320.635 kb on - strand at 2320.657 kb on + strand at 2320.658 kb on - strand at 2320.661 kb on + strand at 2320.661 kb on + strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.891 kb on - strand, within Pf6N2E2_1956 at 2320.899 kb on + strand, within Pf6N2E2_1956 at 2320.899 kb on + strand, within Pf6N2E2_1956 at 2320.900 kb on - strand, within Pf6N2E2_1956 at 2320.900 kb on - strand, within Pf6N2E2_1956 at 2320.901 kb on + strand, within Pf6N2E2_1956 at 2320.902 kb on - strand, within Pf6N2E2_1956 at 2320.928 kb on - strand, within Pf6N2E2_1956 at 2320.981 kb on + strand, within Pf6N2E2_1956 at 2320.981 kb on + strand, within Pf6N2E2_1956 at 2320.982 kb on - strand, within Pf6N2E2_1956 at 2321.049 kb on - strand, within Pf6N2E2_1956 at 2321.087 kb on - strand, within Pf6N2E2_1956 at 2321.102 kb on - strand, within Pf6N2E2_1956 at 2321.159 kb on + strand, within Pf6N2E2_1956 at 2321.160 kb on - strand, within Pf6N2E2_1956 at 2321.160 kb on - strand, within Pf6N2E2_1956 at 2321.276 kb on - strand, within Pf6N2E2_1956 at 2321.276 kb on - strand, within Pf6N2E2_1956 at 2321.419 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.651 kb on - strand at 2321.743 kb on + strand at 2321.743 kb on + strand at 2321.744 kb on - strand at 2321.830 kb on + strand at 2321.830 kb on + strand at 2321.831 kb on - strand at 2321.858 kb on - strand at 2321.858 kb on - strand at 2321.932 kb on - strand, within Pf6N2E2_1957 at 2322.169 kb on - strand, within Pf6N2E2_1957 at 2322.206 kb on + strand, within Pf6N2E2_1957 at 2322.344 kb on + strand, within Pf6N2E2_1957 at 2322.451 kb on + strand, within Pf6N2E2_1957 at 2322.484 kb on + strand, within Pf6N2E2_1957 at 2322.508 kb on + strand, within Pf6N2E2_1957 at 2322.508 kb on + strand, within Pf6N2E2_1957 at 2322.611 kb on + strand, within Pf6N2E2_1957
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 2,319,678 - -1.0 2,319,678 - +0.4 2,319,818 + -0.8 2,319,818 + +0.6 2,319,818 + -0.0 2,319,819 - +1.0 2,320,099 + Pf6N2E2_1955 0.23 +0.3 2,320,099 + Pf6N2E2_1955 0.23 +1.0 2,320,100 - Pf6N2E2_1955 0.23 -0.0 2,320,122 + Pf6N2E2_1955 0.27 +0.6 2,320,122 + Pf6N2E2_1955 0.27 +1.0 2,320,123 - Pf6N2E2_1955 0.27 +0.8 2,320,123 - Pf6N2E2_1955 0.27 -0.6 2,320,124 + Pf6N2E2_1955 0.27 -0.9 2,320,124 + Pf6N2E2_1955 0.27 -0.0 2,320,124 + Pf6N2E2_1955 0.27 +0.8 2,320,124 + Pf6N2E2_1955 0.27 +1.7 2,320,124 + Pf6N2E2_1955 0.27 +0.5 2,320,124 + Pf6N2E2_1955 0.27 -0.6 2,320,124 + Pf6N2E2_1955 0.27 -2.0 2,320,125 - Pf6N2E2_1955 0.28 -0.6 2,320,125 - Pf6N2E2_1955 0.28 -0.1 2,320,125 - Pf6N2E2_1955 0.28 +0.2 2,320,125 - Pf6N2E2_1955 0.28 +0.9 2,320,125 - Pf6N2E2_1955 0.28 -1.0 2,320,164 + Pf6N2E2_1955 0.34 -2.6 2,320,164 + Pf6N2E2_1955 0.34 +2.6 2,320,165 - Pf6N2E2_1955 0.34 -0.2 2,320,165 - Pf6N2E2_1955 0.34 -0.3 2,320,165 - Pf6N2E2_1955 0.34 -1.2 2,320,185 - Pf6N2E2_1955 0.38 -0.2 2,320,246 - Pf6N2E2_1955 0.48 -1.5 2,320,316 + Pf6N2E2_1955 0.59 -1.3 2,320,317 - Pf6N2E2_1955 0.59 -2.9 2,320,573 - -0.2 2,320,576 + +1.6 2,320,577 - -0.0 2,320,577 - +0.3 2,320,577 - +2.1 2,320,577 - +1.0 2,320,577 - -0.4 2,320,590 - -0.6 2,320,635 - +2.0 2,320,657 + -0.6 2,320,658 - +2.7 2,320,661 + -0.7 2,320,661 + -0.6 2,320,662 - -1.8 2,320,662 - -1.3 2,320,662 - +2.4 2,320,662 - -2.0 2,320,662 - +0.4 2,320,891 - Pf6N2E2_1956 0.25 -2.3 2,320,899 + Pf6N2E2_1956 0.26 +1.0 2,320,899 + Pf6N2E2_1956 0.26 -2.0 2,320,900 - Pf6N2E2_1956 0.26 +0.1 2,320,900 - Pf6N2E2_1956 0.26 +0.5 2,320,901 + Pf6N2E2_1956 0.26 +1.0 2,320,902 - Pf6N2E2_1956 0.26 +1.7 2,320,928 - Pf6N2E2_1956 0.28 -1.3 2,320,981 + Pf6N2E2_1956 0.33 +0.6 2,320,981 + Pf6N2E2_1956 0.33 +2.0 2,320,982 - Pf6N2E2_1956 0.33 +1.0 2,321,049 - Pf6N2E2_1956 0.39 +0.5 2,321,087 - Pf6N2E2_1956 0.42 -0.3 2,321,102 - Pf6N2E2_1956 0.43 +1.7 2,321,159 + Pf6N2E2_1956 0.48 +2.1 2,321,160 - Pf6N2E2_1956 0.48 +0.2 2,321,160 - Pf6N2E2_1956 0.48 +1.5 2,321,276 - Pf6N2E2_1956 0.59 -0.6 2,321,276 - Pf6N2E2_1956 0.59 -0.6 2,321,419 + Pf6N2E2_1956 0.71 -1.5 2,321,443 + Pf6N2E2_1956 0.73 -1.0 2,321,443 + Pf6N2E2_1956 0.73 +0.7 2,321,443 + Pf6N2E2_1956 0.73 -0.3 2,321,651 - +0.1 2,321,743 + -1.0 2,321,743 + -0.0 2,321,744 - -0.6 2,321,830 + +1.6 2,321,830 + +1.8 2,321,831 - -0.0 2,321,858 - +0.5 2,321,858 - -1.0 2,321,932 - Pf6N2E2_1957 0.14 +1.2 2,322,169 - Pf6N2E2_1957 0.32 -1.2 2,322,206 + Pf6N2E2_1957 0.35 -1.3 2,322,344 + Pf6N2E2_1957 0.45 -0.0 2,322,451 + Pf6N2E2_1957 0.53 -0.6 2,322,484 + Pf6N2E2_1957 0.56 -0.1 2,322,508 + Pf6N2E2_1957 0.58 -0.6 2,322,508 + Pf6N2E2_1957 0.58 +0.3 2,322,611 + Pf6N2E2_1957 0.65 -0.3
Or see this region's nucleotide sequence