Experiment: Cobalt chloride 0.6 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1660 and Pf6N2E2_1661 are separated by 3 nucleotides Pf6N2E2_1661 and Pf6N2E2_1662 are separated by 132 nucleotides
Pf6N2E2_1660 - RND efflux system, membrane fusion protein CmeA, at 1,964,569 to 1,965,738
_1660
Pf6N2E2_1661 - RND efflux system, inner membrane transporter CmeB, at 1,965,742 to 1,968,837
_1661
Pf6N2E2_1662 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase., at 1,968,970 to 1,969,818
_1662
Position (kb) 1965
1966
1967
1968
1969 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1965.105 kb on + strand, within Pf6N2E2_1660 at 1965.736 kb on + strand at 1965.736 kb on + strand at 1965.737 kb on - strand at 1965.737 kb on - strand at 1965.737 kb on - strand at 1965.877 kb on + strand at 1965.970 kb on + strand at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.485 kb on - strand, within Pf6N2E2_1661 at 1966.562 kb on - strand, within Pf6N2E2_1661 at 1966.684 kb on + strand, within Pf6N2E2_1661 at 1966.685 kb on - strand, within Pf6N2E2_1661 at 1966.705 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1966.762 kb on + strand, within Pf6N2E2_1661 at 1967.137 kb on + strand, within Pf6N2E2_1661 at 1967.138 kb on - strand, within Pf6N2E2_1661 at 1967.368 kb on + strand, within Pf6N2E2_1661 at 1967.369 kb on - strand, within Pf6N2E2_1661 at 1967.446 kb on + strand, within Pf6N2E2_1661 at 1967.446 kb on + strand, within Pf6N2E2_1661 at 1967.494 kb on - strand, within Pf6N2E2_1661 at 1968.038 kb on + strand, within Pf6N2E2_1661 at 1968.178 kb on + strand, within Pf6N2E2_1661 at 1968.178 kb on + strand, within Pf6N2E2_1661 at 1968.178 kb on + strand, within Pf6N2E2_1661 at 1968.398 kb on - strand, within Pf6N2E2_1661 at 1968.461 kb on + strand, within Pf6N2E2_1661 at 1969.222 kb on + strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.223 kb on - strand, within Pf6N2E2_1662 at 1969.409 kb on + strand, within Pf6N2E2_1662 at 1969.410 kb on - strand, within Pf6N2E2_1662 at 1969.410 kb on - strand, within Pf6N2E2_1662 at 1969.521 kb on + strand, within Pf6N2E2_1662 at 1969.522 kb on - strand, within Pf6N2E2_1662 at 1969.810 kb on + strand at 1969.810 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM remove 1,965,105 + Pf6N2E2_1660 0.46 -1.2 1,965,736 + +0.9 1,965,736 + -0.5 1,965,737 - +0.6 1,965,737 - -0.9 1,965,737 - +0.9 1,965,877 + -1.1 1,965,970 + +0.7 1,966,485 - Pf6N2E2_1661 0.24 +0.9 1,966,485 - Pf6N2E2_1661 0.24 -0.4 1,966,485 - Pf6N2E2_1661 0.24 +0.5 1,966,485 - Pf6N2E2_1661 0.24 -0.1 1,966,562 - Pf6N2E2_1661 0.26 -3.0 1,966,684 + Pf6N2E2_1661 0.30 -3.2 1,966,685 - Pf6N2E2_1661 0.30 -2.1 1,966,705 + Pf6N2E2_1661 0.31 -1.1 1,966,762 + Pf6N2E2_1661 0.33 -0.1 1,966,762 + Pf6N2E2_1661 0.33 -3.4 1,966,762 + Pf6N2E2_1661 0.33 -1.4 1,966,762 + Pf6N2E2_1661 0.33 -0.1 1,966,762 + Pf6N2E2_1661 0.33 -0.7 1,966,762 + Pf6N2E2_1661 0.33 -1.1 1,967,137 + Pf6N2E2_1661 0.45 +0.5 1,967,138 - Pf6N2E2_1661 0.45 -1.3 1,967,368 + Pf6N2E2_1661 0.53 -0.9 1,967,369 - Pf6N2E2_1661 0.53 -0.7 1,967,446 + Pf6N2E2_1661 0.55 -2.2 1,967,446 + Pf6N2E2_1661 0.55 -1.5 1,967,494 - Pf6N2E2_1661 0.57 +0.9 1,968,038 + Pf6N2E2_1661 0.74 -0.9 1,968,178 + Pf6N2E2_1661 0.79 -2.5 1,968,178 + Pf6N2E2_1661 0.79 +1.2 1,968,178 + Pf6N2E2_1661 0.79 -1.1 1,968,398 - Pf6N2E2_1661 0.86 -1.1 1,968,461 + Pf6N2E2_1661 0.88 -1.4 1,969,222 + Pf6N2E2_1662 0.30 -1.8 1,969,223 - Pf6N2E2_1662 0.30 -0.1 1,969,223 - Pf6N2E2_1662 0.30 -0.6 1,969,223 - Pf6N2E2_1662 0.30 -1.1 1,969,409 + Pf6N2E2_1662 0.52 -0.1 1,969,410 - Pf6N2E2_1662 0.52 +2.3 1,969,410 - Pf6N2E2_1662 0.52 +0.6 1,969,521 + Pf6N2E2_1662 0.65 +0.1 1,969,522 - Pf6N2E2_1662 0.65 +0.9 1,969,810 + +0.7 1,969,810 + -0.1
Or see this region's nucleotide sequence