Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1123

Experiment: Cobalt chloride 0.6 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1121 and Pf6N2E2_1122 are separated by 64 nucleotidesPf6N2E2_1122 and Pf6N2E2_1123 are separated by 13 nucleotidesPf6N2E2_1123 and Pf6N2E2_1124 overlap by 4 nucleotidesPf6N2E2_1124 and Pf6N2E2_1125 are separated by 142 nucleotides Pf6N2E2_1121 - 3-phytase precursor (EC 3.1.3.8), at 1,316,791 to 1,318,734 _1121 Pf6N2E2_1122 - MotA/TolQ/ExbB proton channel family protein, at 1,318,799 to 1,319,506 _1122 Pf6N2E2_1123 - Tol biopolymer transport system, TolR protein, at 1,319,520 to 1,319,966 _1123 Pf6N2E2_1124 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,319,963 to 1,320,778 _1124 Pf6N2E2_1125 - Transcriptional regulator, AraC family, at 1,320,921 to 1,321,817 _1125 Position (kb) 1319 1320Strain fitness (log2 ratio) -2 -1 0 1at 1318.775 kb on - strandat 1318.775 kb on - strandat 1318.907 kb on + strand, within Pf6N2E2_1122at 1318.908 kb on - strand, within Pf6N2E2_1122at 1319.006 kb on + strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.170 kb on - strand, within Pf6N2E2_1122at 1319.223 kb on - strandat 1319.381 kb on + strand, within Pf6N2E2_1122at 1319.381 kb on + strand, within Pf6N2E2_1122at 1319.427 kb on - strand, within Pf6N2E2_1122at 1319.427 kb on - strand, within Pf6N2E2_1122at 1319.427 kb on - strand, within Pf6N2E2_1122at 1319.577 kb on + strand, within Pf6N2E2_1123at 1319.577 kb on + strand, within Pf6N2E2_1123at 1319.737 kb on - strand, within Pf6N2E2_1123at 1319.739 kb on + strand, within Pf6N2E2_1123at 1319.739 kb on + strand, within Pf6N2E2_1123at 1319.740 kb on - strand, within Pf6N2E2_1123at 1319.740 kb on - strand, within Pf6N2E2_1123at 1319.740 kb on - strand, within Pf6N2E2_1123at 1319.856 kb on + strand, within Pf6N2E2_1123at 1319.857 kb on - strand, within Pf6N2E2_1123at 1319.857 kb on - strand, within Pf6N2E2_1123at 1320.207 kb on + strand, within Pf6N2E2_1124at 1320.599 kb on + strand, within Pf6N2E2_1124at 1320.600 kb on - strand, within Pf6N2E2_1124at 1320.600 kb on - strand, within Pf6N2E2_1124at 1320.754 kb on + strandat 1320.755 kb on - strandat 1320.789 kb on + strandat 1320.895 kb on - strandat 1320.922 kb on + strandat 1320.922 kb on + strandat 1320.922 kb on + strandat 1320.923 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.6 mM
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1,318,775 - +0.4
1,318,775 - -1.1
1,318,907 + Pf6N2E2_1122 0.15 -0.2
1,318,908 - Pf6N2E2_1122 0.15 +0.8
1,319,006 + Pf6N2E2_1122 0.29 +0.3
1,319,170 - Pf6N2E2_1122 0.52 +0.7
1,319,170 - Pf6N2E2_1122 0.52 -0.7
1,319,170 - Pf6N2E2_1122 0.52 -0.4
1,319,170 - Pf6N2E2_1122 0.52 +0.4
1,319,223 - +0.8
1,319,381 + Pf6N2E2_1122 0.82 +0.1
1,319,381 + Pf6N2E2_1122 0.82 +0.4
1,319,427 - Pf6N2E2_1122 0.89 +0.6
1,319,427 - Pf6N2E2_1122 0.89 +0.5
1,319,427 - Pf6N2E2_1122 0.89 +0.3
1,319,577 + Pf6N2E2_1123 0.13 -0.5
1,319,577 + Pf6N2E2_1123 0.13 -0.3
1,319,737 - Pf6N2E2_1123 0.49 -1.7
1,319,739 + Pf6N2E2_1123 0.49 -1.5
1,319,739 + Pf6N2E2_1123 0.49 +0.5
1,319,740 - Pf6N2E2_1123 0.49 -1.5
1,319,740 - Pf6N2E2_1123 0.49 -1.7
1,319,740 - Pf6N2E2_1123 0.49 +1.1
1,319,856 + Pf6N2E2_1123 0.75 -2.3
1,319,857 - Pf6N2E2_1123 0.75 -2.0
1,319,857 - Pf6N2E2_1123 0.75 -1.6
1,320,207 + Pf6N2E2_1124 0.30 +0.8
1,320,599 + Pf6N2E2_1124 0.78 -0.2
1,320,600 - Pf6N2E2_1124 0.78 -1.5
1,320,600 - Pf6N2E2_1124 0.78 +0.3
1,320,754 + +0.8
1,320,755 - -0.7
1,320,789 + -0.7
1,320,895 - -0.5
1,320,922 + -0.7
1,320,922 + +0.0
1,320,922 + +0.8
1,320,923 - +1.3

Or see this region's nucleotide sequence