Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_795 and Pf6N2E2_796 are separated by 428 nucleotides Pf6N2E2_796 and Pf6N2E2_797 are separated by 15 nucleotides
Pf6N2E2_795 - ABC transporter ATP-binding protein uup, at 925,389 to 926,954
_795
Pf6N2E2_796 - Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO, at 927,383 to 928,276
_796
Pf6N2E2_797 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 928,292 to 930,259
_797
Position (kb)
927
928
929 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 926.594 kb on - strand, within Pf6N2E2_795 at 926.964 kb on - strand at 926.964 kb on - strand at 926.964 kb on - strand at 926.969 kb on + strand at 926.970 kb on - strand at 926.970 kb on - strand at 926.984 kb on + strand at 927.006 kb on - strand at 927.006 kb on - strand at 927.009 kb on + strand at 927.009 kb on + strand at 927.010 kb on - strand at 927.023 kb on + strand at 927.035 kb on - strand at 927.102 kb on - strand at 927.119 kb on - strand at 927.138 kb on - strand at 927.185 kb on + strand at 927.186 kb on - strand at 927.186 kb on - strand at 927.188 kb on + strand at 927.188 kb on + strand at 927.188 kb on + strand at 927.189 kb on - strand at 927.189 kb on - strand at 927.189 kb on - strand at 927.205 kb on - strand at 927.205 kb on - strand at 927.232 kb on + strand at 927.233 kb on - strand at 927.233 kb on - strand at 927.293 kb on + strand at 927.294 kb on - strand at 927.407 kb on + strand at 927.407 kb on + strand at 927.407 kb on + strand at 927.407 kb on + strand at 927.407 kb on + strand at 927.408 kb on - strand at 927.408 kb on - strand at 927.408 kb on - strand at 927.425 kb on + strand at 927.426 kb on - strand at 927.455 kb on - strand at 927.515 kb on + strand, within Pf6N2E2_796 at 927.515 kb on + strand, within Pf6N2E2_796 at 927.515 kb on + strand, within Pf6N2E2_796 at 927.515 kb on + strand, within Pf6N2E2_796 at 927.516 kb on - strand, within Pf6N2E2_796 at 927.753 kb on + strand, within Pf6N2E2_796 at 927.753 kb on + strand, within Pf6N2E2_796 at 927.753 kb on + strand, within Pf6N2E2_796 at 927.754 kb on - strand, within Pf6N2E2_796 at 927.754 kb on - strand, within Pf6N2E2_796 at 927.771 kb on + strand, within Pf6N2E2_796 at 927.852 kb on - strand, within Pf6N2E2_796 at 927.908 kb on + strand, within Pf6N2E2_796 at 927.908 kb on + strand, within Pf6N2E2_796 at 927.909 kb on - strand, within Pf6N2E2_796 at 927.932 kb on + strand, within Pf6N2E2_796 at 927.932 kb on + strand, within Pf6N2E2_796 at 927.983 kb on + strand, within Pf6N2E2_796 at 928.006 kb on + strand, within Pf6N2E2_796 at 928.007 kb on - strand, within Pf6N2E2_796 at 928.166 kb on + strand, within Pf6N2E2_796 at 928.174 kb on - strand, within Pf6N2E2_796 at 928.217 kb on + strand at 928.217 kb on + strand at 928.217 kb on + strand at 928.217 kb on + strand at 928.218 kb on - strand at 928.220 kb on + strand at 928.221 kb on - strand at 928.264 kb on + strand at 928.265 kb on - strand at 928.265 kb on - strand at 928.287 kb on + strand at 928.288 kb on - strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.410 kb on + strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.411 kb on - strand at 928.543 kb on - strand, within Pf6N2E2_797 at 928.630 kb on + strand, within Pf6N2E2_797 at 928.630 kb on + strand, within Pf6N2E2_797 at 928.631 kb on - strand, within Pf6N2E2_797 at 929.103 kb on + strand, within Pf6N2E2_797 at 929.104 kb on - strand, within Pf6N2E2_797 at 929.104 kb on - strand, within Pf6N2E2_797 at 929.104 kb on - strand, within Pf6N2E2_797 at 929.104 kb on - strand, within Pf6N2E2_797 at 929.104 kb on - strand, within Pf6N2E2_797 at 929.106 kb on + strand, within Pf6N2E2_797
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 926,594 - Pf6N2E2_795 0.77 +0.7 926,964 - +0.7 926,964 - -0.9 926,964 - -0.7 926,969 + -0.3 926,970 - -0.8 926,970 - -0.9 926,984 + +0.7 927,006 - -0.3 927,006 - -0.9 927,009 + +0.9 927,009 + +1.7 927,010 - +1.8 927,023 + -0.3 927,035 - +1.7 927,102 - +0.7 927,119 - +0.1 927,138 - +0.8 927,185 + +1.0 927,186 - +1.2 927,186 - +1.4 927,188 + +0.3 927,188 + -0.1 927,188 + -1.8 927,189 - +1.4 927,189 - +0.2 927,189 - +1.1 927,205 - +1.7 927,205 - +0.7 927,232 + +0.4 927,233 - -0.0 927,233 - +0.4 927,293 + +2.7 927,294 - -1.3 927,407 + +0.7 927,407 + +0.4 927,407 + -1.7 927,407 + -0.2 927,407 + +0.7 927,408 - +0.7 927,408 - +0.7 927,408 - +0.7 927,425 + +0.7 927,426 - -0.9 927,455 - -0.9 927,515 + Pf6N2E2_796 0.15 -0.9 927,515 + Pf6N2E2_796 0.15 -0.2 927,515 + Pf6N2E2_796 0.15 -0.6 927,515 + Pf6N2E2_796 0.15 -0.7 927,516 - Pf6N2E2_796 0.15 +0.7 927,753 + Pf6N2E2_796 0.41 -0.7 927,753 + Pf6N2E2_796 0.41 +1.0 927,753 + Pf6N2E2_796 0.41 +0.9 927,754 - Pf6N2E2_796 0.41 -0.1 927,754 - Pf6N2E2_796 0.41 -1.9 927,771 + Pf6N2E2_796 0.43 -1.2 927,852 - Pf6N2E2_796 0.52 -0.9 927,908 + Pf6N2E2_796 0.59 -2.2 927,908 + Pf6N2E2_796 0.59 +0.7 927,909 - Pf6N2E2_796 0.59 +0.7 927,932 + Pf6N2E2_796 0.61 +2.2 927,932 + Pf6N2E2_796 0.61 -0.7 927,983 + Pf6N2E2_796 0.67 -1.5 928,006 + Pf6N2E2_796 0.70 -1.1 928,007 - Pf6N2E2_796 0.70 -0.1 928,166 + Pf6N2E2_796 0.88 -0.1 928,174 - Pf6N2E2_796 0.88 -0.1 928,217 + +1.7 928,217 + +2.7 928,217 + +0.5 928,217 + +0.5 928,218 - +0.4 928,220 + +1.2 928,221 - -1.9 928,264 + -0.2 928,265 - -0.8 928,265 - +0.4 928,287 + -0.9 928,288 - +0.9 928,410 + -2.2 928,410 + -0.5 928,410 + -0.7 928,410 + -0.3 928,410 + -0.7 928,410 + -0.3 928,410 + -0.4 928,410 + +0.7 928,410 + +0.7 928,411 - -0.3 928,411 - -0.7 928,411 - +2.7 928,411 - +0.7 928,411 - -0.3 928,411 - -0.3 928,411 - -1.3 928,411 - -2.2 928,543 - Pf6N2E2_797 0.13 -0.0 928,630 + Pf6N2E2_797 0.17 +1.2 928,630 + Pf6N2E2_797 0.17 -0.3 928,631 - Pf6N2E2_797 0.17 -1.2 929,103 + Pf6N2E2_797 0.41 +0.5 929,104 - Pf6N2E2_797 0.41 -0.6 929,104 - Pf6N2E2_797 0.41 +0.7 929,104 - Pf6N2E2_797 0.41 +0.3 929,104 - Pf6N2E2_797 0.41 +0.3 929,104 - Pf6N2E2_797 0.41 +0.9 929,106 + Pf6N2E2_797 0.41 +0.5
Or see this region's nucleotide sequence