Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5384 and Pf6N2E2_5385 overlap by 4 nucleotides Pf6N2E2_5385 and Pf6N2E2_5386 are separated by 99 nucleotides
Pf6N2E2_5384 - outer membrane protein AlgE, at 6,187,445 to 6,188,944
_5384
Pf6N2E2_5385 - Alginate biosynthesis protein AlgK precursor, at 6,188,941 to 6,190,341
_5385
Pf6N2E2_5386 - Alginate biosynthesis protein Alg44, at 6,190,441 to 6,191,610
_5386
Position (kb)
6188
6189
6190
6191 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6187.941 kb on + strand, within Pf6N2E2_5384 at 6187.962 kb on + strand, within Pf6N2E2_5384 at 6188.130 kb on + strand, within Pf6N2E2_5384 at 6188.131 kb on - strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.160 kb on + strand, within Pf6N2E2_5384 at 6188.251 kb on - strand, within Pf6N2E2_5384 at 6188.698 kb on - strand, within Pf6N2E2_5384 at 6188.730 kb on + strand, within Pf6N2E2_5384 at 6188.731 kb on - strand, within Pf6N2E2_5384 at 6188.731 kb on - strand, within Pf6N2E2_5384 at 6188.944 kb on + strand at 6188.971 kb on + strand at 6189.031 kb on + strand at 6189.031 kb on + strand at 6189.031 kb on + strand at 6189.068 kb on - strand at 6189.143 kb on + strand, within Pf6N2E2_5385 at 6189.143 kb on + strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.144 kb on - strand, within Pf6N2E2_5385 at 6189.266 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.293 kb on + strand, within Pf6N2E2_5385 at 6189.294 kb on - strand, within Pf6N2E2_5385 at 6189.308 kb on + strand, within Pf6N2E2_5385 at 6189.309 kb on - strand, within Pf6N2E2_5385 at 6189.309 kb on - strand, within Pf6N2E2_5385 at 6189.311 kb on + strand, within Pf6N2E2_5385 at 6189.395 kb on + strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.396 kb on - strand, within Pf6N2E2_5385 at 6189.398 kb on + strand, within Pf6N2E2_5385 at 6189.399 kb on - strand, within Pf6N2E2_5385 at 6189.458 kb on + strand, within Pf6N2E2_5385 at 6189.458 kb on + strand, within Pf6N2E2_5385 at 6189.459 kb on - strand, within Pf6N2E2_5385 at 6189.501 kb on - strand, within Pf6N2E2_5385 at 6189.719 kb on + strand, within Pf6N2E2_5385 at 6189.720 kb on - strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.740 kb on + strand, within Pf6N2E2_5385 at 6189.741 kb on - strand, within Pf6N2E2_5385 at 6189.806 kb on + strand, within Pf6N2E2_5385 at 6189.806 kb on + strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.807 kb on - strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.929 kb on + strand, within Pf6N2E2_5385 at 6189.930 kb on - strand, within Pf6N2E2_5385 at 6189.930 kb on - strand, within Pf6N2E2_5385 at 6190.176 kb on - strand, within Pf6N2E2_5385 at 6190.199 kb on + strand, within Pf6N2E2_5385 at 6190.199 kb on + strand, within Pf6N2E2_5385 at 6190.199 kb on + strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.200 kb on - strand, within Pf6N2E2_5385 at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.341 kb on + strand at 6190.342 kb on - strand at 6190.342 kb on - strand at 6190.342 kb on - strand at 6190.414 kb on + strand at 6190.415 kb on - strand at 6190.416 kb on + strand at 6190.416 kb on + strand at 6190.416 kb on + strand at 6190.431 kb on + strand at 6190.432 kb on - strand at 6190.432 kb on - strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.442 kb on + strand at 6190.446 kb on + strand at 6190.446 kb on + strand at 6190.511 kb on - strand at 6190.665 kb on + strand, within Pf6N2E2_5386 at 6191.004 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.057 kb on + strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.058 kb on - strand, within Pf6N2E2_5386 at 6191.067 kb on - strand, within Pf6N2E2_5386
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 6,187,941 + Pf6N2E2_5384 0.33 +0.4 6,187,962 + Pf6N2E2_5384 0.34 +1.4 6,188,130 + Pf6N2E2_5384 0.46 +1.1 6,188,131 - Pf6N2E2_5384 0.46 -0.6 6,188,160 + Pf6N2E2_5384 0.48 +0.5 6,188,160 + Pf6N2E2_5384 0.48 -0.2 6,188,160 + Pf6N2E2_5384 0.48 -0.5 6,188,160 + Pf6N2E2_5384 0.48 -0.7 6,188,251 - Pf6N2E2_5384 0.54 -0.6 6,188,698 - Pf6N2E2_5384 0.84 +1.1 6,188,730 + Pf6N2E2_5384 0.86 +1.5 6,188,731 - Pf6N2E2_5384 0.86 +0.7 6,188,731 - Pf6N2E2_5384 0.86 -0.9 6,188,944 + +0.6 6,188,971 + +0.6 6,189,031 + +0.5 6,189,031 + +0.8 6,189,031 + +0.9 6,189,068 - -1.7 6,189,143 + Pf6N2E2_5385 0.14 -0.9 6,189,143 + Pf6N2E2_5385 0.14 -0.7 6,189,144 - Pf6N2E2_5385 0.14 -0.6 6,189,144 - Pf6N2E2_5385 0.14 +0.2 6,189,144 - Pf6N2E2_5385 0.14 -1.1 6,189,144 - Pf6N2E2_5385 0.14 +0.5 6,189,144 - Pf6N2E2_5385 0.14 -0.7 6,189,144 - Pf6N2E2_5385 0.14 -0.2 6,189,144 - Pf6N2E2_5385 0.14 -0.1 6,189,144 - Pf6N2E2_5385 0.14 -1.9 6,189,266 + Pf6N2E2_5385 0.23 +0.5 6,189,293 + Pf6N2E2_5385 0.25 +2.1 6,189,293 + Pf6N2E2_5385 0.25 -0.8 6,189,293 + Pf6N2E2_5385 0.25 -0.2 6,189,293 + Pf6N2E2_5385 0.25 -0.9 6,189,294 - Pf6N2E2_5385 0.25 +0.9 6,189,308 + Pf6N2E2_5385 0.26 +0.4 6,189,309 - Pf6N2E2_5385 0.26 -1.4 6,189,309 - Pf6N2E2_5385 0.26 +1.1 6,189,311 + Pf6N2E2_5385 0.26 +0.5 6,189,395 + Pf6N2E2_5385 0.32 -0.5 6,189,396 - Pf6N2E2_5385 0.32 -0.3 6,189,396 - Pf6N2E2_5385 0.32 +0.1 6,189,396 - Pf6N2E2_5385 0.32 -2.5 6,189,396 - Pf6N2E2_5385 0.32 +0.2 6,189,398 + Pf6N2E2_5385 0.33 -0.8 6,189,399 - Pf6N2E2_5385 0.33 -0.5 6,189,458 + Pf6N2E2_5385 0.37 -0.0 6,189,458 + Pf6N2E2_5385 0.37 -1.6 6,189,459 - Pf6N2E2_5385 0.37 -0.5 6,189,501 - Pf6N2E2_5385 0.40 -1.1 6,189,719 + Pf6N2E2_5385 0.56 -0.5 6,189,720 - Pf6N2E2_5385 0.56 +0.5 6,189,740 + Pf6N2E2_5385 0.57 -0.0 6,189,740 + Pf6N2E2_5385 0.57 +0.0 6,189,740 + Pf6N2E2_5385 0.57 +0.1 6,189,741 - Pf6N2E2_5385 0.57 +0.3 6,189,806 + Pf6N2E2_5385 0.62 -0.3 6,189,806 + Pf6N2E2_5385 0.62 -0.8 6,189,807 - Pf6N2E2_5385 0.62 -0.7 6,189,807 - Pf6N2E2_5385 0.62 -0.4 6,189,807 - Pf6N2E2_5385 0.62 +0.6 6,189,807 - Pf6N2E2_5385 0.62 -0.5 6,189,929 + Pf6N2E2_5385 0.71 -0.7 6,189,929 + Pf6N2E2_5385 0.71 -0.0 6,189,929 + Pf6N2E2_5385 0.71 -2.2 6,189,930 - Pf6N2E2_5385 0.71 -0.9 6,189,930 - Pf6N2E2_5385 0.71 +0.4 6,190,176 - Pf6N2E2_5385 0.88 -0.7 6,190,199 + Pf6N2E2_5385 0.90 -0.5 6,190,199 + Pf6N2E2_5385 0.90 +0.9 6,190,199 + Pf6N2E2_5385 0.90 +0.8 6,190,200 - Pf6N2E2_5385 0.90 -1.6 6,190,200 - Pf6N2E2_5385 0.90 -0.6 6,190,200 - Pf6N2E2_5385 0.90 +0.0 6,190,200 - Pf6N2E2_5385 0.90 -1.3 6,190,200 - Pf6N2E2_5385 0.90 +0.1 6,190,200 - Pf6N2E2_5385 0.90 -0.4 6,190,200 - Pf6N2E2_5385 0.90 -0.3 6,190,200 - Pf6N2E2_5385 0.90 -2.9 6,190,341 + +0.2 6,190,341 + -0.2 6,190,341 + -0.0 6,190,341 + +0.2 6,190,341 + +0.7 6,190,342 - -0.7 6,190,342 - -0.0 6,190,342 - +0.0 6,190,414 + -1.9 6,190,415 - +0.8 6,190,416 + -0.7 6,190,416 + -0.3 6,190,416 + -0.4 6,190,431 + +0.3 6,190,432 - -0.0 6,190,432 - -0.3 6,190,442 + +0.4 6,190,442 + +0.3 6,190,442 + +0.1 6,190,442 + -0.4 6,190,442 + +1.1 6,190,442 + +0.1 6,190,446 + -0.3 6,190,446 + -0.7 6,190,511 - +0.2 6,190,665 + Pf6N2E2_5386 0.19 -0.4 6,191,004 + Pf6N2E2_5386 0.48 -0.9 6,191,057 + Pf6N2E2_5386 0.53 -1.5 6,191,057 + Pf6N2E2_5386 0.53 +0.6 6,191,057 + Pf6N2E2_5386 0.53 -2.1 6,191,057 + Pf6N2E2_5386 0.53 +0.8 6,191,057 + Pf6N2E2_5386 0.53 -1.7 6,191,057 + Pf6N2E2_5386 0.53 -1.3 6,191,057 + Pf6N2E2_5386 0.53 -0.5 6,191,057 + Pf6N2E2_5386 0.53 +1.1 6,191,057 + Pf6N2E2_5386 0.53 +0.3 6,191,058 - Pf6N2E2_5386 0.53 +0.4 6,191,058 - Pf6N2E2_5386 0.53 -1.2 6,191,058 - Pf6N2E2_5386 0.53 -2.2 6,191,058 - Pf6N2E2_5386 0.53 -2.0 6,191,058 - Pf6N2E2_5386 0.53 -0.7 6,191,058 - Pf6N2E2_5386 0.53 +0.8 6,191,067 - Pf6N2E2_5386 0.54 -2.1
Or see this region's nucleotide sequence