Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3544

Experiment: Cobalt chloride 0.4 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3543 and Pf6N2E2_3544 are separated by 115 nucleotidesPf6N2E2_3544 and Pf6N2E2_3545 overlap by 4 nucleotidesPf6N2E2_3545 and Pf6N2E2_3546 overlap by 4 nucleotides Pf6N2E2_3543 - Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB, at 4,133,587 to 4,134,966 _3543 Pf6N2E2_3544 - ABC transporter (iron.B12.siderophore.hemin) , ATP-binding component, at 4,135,082 to 4,135,870 _3544 Pf6N2E2_3545 - ABC transporter (iron.B12.siderophore.hemin) , periplasmic substrate-binding component, at 4,135,867 to 4,136,814 _3545 Pf6N2E2_3546 - ABC transporter (iron.B12.siderophore.hemin) , permease component, at 4,136,811 to 4,137,821 _3546 Position (kb) 4135 4136Strain fitness (log2 ratio) -2 -1 0 1at 4134.242 kb on - strand, within Pf6N2E2_3543at 4134.493 kb on + strand, within Pf6N2E2_3543at 4134.589 kb on + strand, within Pf6N2E2_3543at 4134.589 kb on + strand, within Pf6N2E2_3543at 4134.589 kb on + strand, within Pf6N2E2_3543at 4134.589 kb on + strand, within Pf6N2E2_3543at 4134.589 kb on + strand, within Pf6N2E2_3543at 4134.590 kb on - strand, within Pf6N2E2_3543at 4134.590 kb on - strand, within Pf6N2E2_3543at 4135.002 kb on + strandat 4135.003 kb on - strandat 4135.067 kb on + strandat 4135.068 kb on - strandat 4135.068 kb on - strandat 4135.077 kb on + strandat 4135.077 kb on + strandat 4135.077 kb on + strandat 4135.078 kb on - strandat 4135.078 kb on - strandat 4135.078 kb on - strandat 4135.078 kb on - strandat 4135.078 kb on - strandat 4135.093 kb on - strandat 4135.247 kb on + strand, within Pf6N2E2_3544at 4135.278 kb on + strand, within Pf6N2E2_3544at 4135.278 kb on + strand, within Pf6N2E2_3544at 4135.279 kb on - strand, within Pf6N2E2_3544at 4135.279 kb on - strand, within Pf6N2E2_3544at 4135.415 kb on - strand, within Pf6N2E2_3544at 4135.619 kb on + strand, within Pf6N2E2_3544at 4135.619 kb on + strand, within Pf6N2E2_3544at 4135.620 kb on - strand, within Pf6N2E2_3544at 4135.718 kb on + strand, within Pf6N2E2_3544at 4135.719 kb on - strand, within Pf6N2E2_3544at 4136.069 kb on - strand, within Pf6N2E2_3545at 4136.150 kb on - strand, within Pf6N2E2_3545at 4136.150 kb on - strand, within Pf6N2E2_3545at 4136.272 kb on + strand, within Pf6N2E2_3545at 4136.331 kb on + strand, within Pf6N2E2_3545at 4136.332 kb on - strand, within Pf6N2E2_3545at 4136.335 kb on + strand, within Pf6N2E2_3545at 4136.335 kb on + strand, within Pf6N2E2_3545at 4136.335 kb on + strand, within Pf6N2E2_3545at 4136.336 kb on - strand, within Pf6N2E2_3545at 4136.336 kb on - strand, within Pf6N2E2_3545at 4136.336 kb on - strand, within Pf6N2E2_3545at 4136.336 kb on - strand, within Pf6N2E2_3545at 4136.336 kb on - strand, within Pf6N2E2_3545at 4136.336 kb on - strand, within Pf6N2E2_3545at 4136.550 kb on + strand, within Pf6N2E2_3545at 4136.600 kb on + strand, within Pf6N2E2_3545at 4136.600 kb on + strand, within Pf6N2E2_3545at 4136.601 kb on - strand, within Pf6N2E2_3545at 4136.740 kb on + strandat 4136.741 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM
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4,134,242 - Pf6N2E2_3543 0.47 +0.1
4,134,493 + Pf6N2E2_3543 0.66 -0.4
4,134,589 + Pf6N2E2_3543 0.73 +0.4
4,134,589 + Pf6N2E2_3543 0.73 +0.6
4,134,589 + Pf6N2E2_3543 0.73 -0.6
4,134,589 + Pf6N2E2_3543 0.73 +0.9
4,134,589 + Pf6N2E2_3543 0.73 -0.1
4,134,590 - Pf6N2E2_3543 0.73 +1.2
4,134,590 - Pf6N2E2_3543 0.73 +1.2
4,135,002 + +0.4
4,135,003 - -0.8
4,135,067 + +1.1
4,135,068 - +0.1
4,135,068 - +0.2
4,135,077 + -0.6
4,135,077 + -0.1
4,135,077 + +0.5
4,135,078 - -0.6
4,135,078 - +0.4
4,135,078 - -0.2
4,135,078 - -0.9
4,135,078 - +0.2
4,135,093 - +0.0
4,135,247 + Pf6N2E2_3544 0.21 -1.1
4,135,278 + Pf6N2E2_3544 0.25 +0.2
4,135,278 + Pf6N2E2_3544 0.25 +1.2
4,135,279 - Pf6N2E2_3544 0.25 -1.8
4,135,279 - Pf6N2E2_3544 0.25 -1.4
4,135,415 - Pf6N2E2_3544 0.42 +0.4
4,135,619 + Pf6N2E2_3544 0.68 -0.4
4,135,619 + Pf6N2E2_3544 0.68 -0.8
4,135,620 - Pf6N2E2_3544 0.68 -0.3
4,135,718 + Pf6N2E2_3544 0.81 -1.1
4,135,719 - Pf6N2E2_3544 0.81 -1.6
4,136,069 - Pf6N2E2_3545 0.21 -1.1
4,136,150 - Pf6N2E2_3545 0.30 -0.2
4,136,150 - Pf6N2E2_3545 0.30 -0.1
4,136,272 + Pf6N2E2_3545 0.43 -0.7
4,136,331 + Pf6N2E2_3545 0.49 -0.4
4,136,332 - Pf6N2E2_3545 0.49 -0.9
4,136,335 + Pf6N2E2_3545 0.49 +0.6
4,136,335 + Pf6N2E2_3545 0.49 -1.2
4,136,335 + Pf6N2E2_3545 0.49 +1.2
4,136,336 - Pf6N2E2_3545 0.49 -0.4
4,136,336 - Pf6N2E2_3545 0.49 +0.1
4,136,336 - Pf6N2E2_3545 0.49 +0.6
4,136,336 - Pf6N2E2_3545 0.49 -1.1
4,136,336 - Pf6N2E2_3545 0.49 +0.4
4,136,336 - Pf6N2E2_3545 0.49 -0.6
4,136,550 + Pf6N2E2_3545 0.72 -1.5
4,136,600 + Pf6N2E2_3545 0.77 -0.0
4,136,600 + Pf6N2E2_3545 0.77 +0.2
4,136,601 - Pf6N2E2_3545 0.77 -0.1
4,136,740 + +1.2
4,136,741 - -0.5

Or see this region's nucleotide sequence