Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2357 and Pf6N2E2_2358 are separated by 713 nucleotides Pf6N2E2_2358 and Pf6N2E2_2359 are separated by 10 nucleotides
Pf6N2E2_2357 - Invasion protein iagA, at 2,790,348 to 2,791,409
_2357
Pf6N2E2_2358 - Type III secretion host injection protein (YopB), at 2,792,123 to 2,793,223
_2358
Pf6N2E2_2359 - hypothetical protein, at 2,793,234 to 2,794,343
_2359
Position (kb)
2792
2793
2794 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2791.322 kb on - strand at 2791.337 kb on - strand at 2791.337 kb on - strand at 2791.377 kb on - strand at 2791.377 kb on - strand at 2791.419 kb on + strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.420 kb on - strand at 2791.482 kb on + strand at 2791.482 kb on + strand at 2791.608 kb on - strand at 2791.625 kb on + strand at 2791.686 kb on + strand at 2791.728 kb on + strand at 2791.728 kb on + strand at 2791.729 kb on - strand at 2791.736 kb on + strand at 2791.751 kb on + strand at 2791.752 kb on - strand at 2791.752 kb on - strand at 2791.930 kb on - strand at 2791.930 kb on - strand at 2792.014 kb on + strand at 2792.015 kb on - strand at 2792.015 kb on - strand at 2792.015 kb on - strand at 2792.015 kb on - strand at 2792.015 kb on - strand at 2792.056 kb on + strand at 2792.077 kb on + strand at 2792.077 kb on + strand at 2792.077 kb on + strand at 2792.078 kb on - strand at 2792.078 kb on - strand at 2792.078 kb on - strand at 2792.220 kb on + strand at 2792.221 kb on - strand at 2792.296 kb on - strand, within Pf6N2E2_2358 at 2792.345 kb on + strand, within Pf6N2E2_2358 at 2792.345 kb on + strand, within Pf6N2E2_2358 at 2792.468 kb on - strand, within Pf6N2E2_2358 at 2792.504 kb on - strand, within Pf6N2E2_2358 at 2792.542 kb on + strand, within Pf6N2E2_2358 at 2792.543 kb on - strand, within Pf6N2E2_2358 at 2792.543 kb on - strand, within Pf6N2E2_2358 at 2792.559 kb on - strand, within Pf6N2E2_2358 at 2792.630 kb on - strand, within Pf6N2E2_2358 at 2792.633 kb on + strand, within Pf6N2E2_2358 at 2792.634 kb on - strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.664 kb on + strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.665 kb on - strand, within Pf6N2E2_2358 at 2792.698 kb on - strand, within Pf6N2E2_2358 at 2793.126 kb on - strand at 2793.126 kb on - strand at 2793.204 kb on - strand at 2793.410 kb on - strand, within Pf6N2E2_2359 at 2793.561 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.667 kb on + strand, within Pf6N2E2_2359 at 2793.668 kb on - strand, within Pf6N2E2_2359 at 2793.668 kb on - strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2793.934 kb on + strand, within Pf6N2E2_2359 at 2794.112 kb on + strand at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.115 kb on + strand, within Pf6N2E2_2359 at 2794.116 kb on - strand, within Pf6N2E2_2359 at 2794.116 kb on - strand, within Pf6N2E2_2359
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 2,791,322 - +1.5 2,791,337 - +0.6 2,791,337 - +0.2 2,791,377 - +0.1 2,791,377 - +0.3 2,791,419 + -0.8 2,791,420 - -1.0 2,791,420 - -0.5 2,791,420 - -1.2 2,791,420 - -0.2 2,791,420 - -0.4 2,791,420 - -0.4 2,791,420 - -1.0 2,791,420 - -0.7 2,791,420 - +0.5 2,791,420 - -1.9 2,791,482 + +0.8 2,791,482 + +1.4 2,791,608 - -0.3 2,791,625 + -0.2 2,791,686 + -2.0 2,791,728 + -1.8 2,791,728 + +0.8 2,791,729 - +0.6 2,791,736 + +1.6 2,791,751 + -0.4 2,791,752 - +0.8 2,791,752 - +1.8 2,791,930 - -0.5 2,791,930 - -0.8 2,792,014 + +2.4 2,792,015 - -1.1 2,792,015 - -0.4 2,792,015 - -1.1 2,792,015 - +0.9 2,792,015 - -0.0 2,792,056 + -0.2 2,792,077 + -0.2 2,792,077 + +0.2 2,792,077 + -0.5 2,792,078 - +0.1 2,792,078 - -0.9 2,792,078 - +0.1 2,792,220 + -0.4 2,792,221 - -1.5 2,792,296 - Pf6N2E2_2358 0.16 -0.2 2,792,345 + Pf6N2E2_2358 0.20 -2.4 2,792,345 + Pf6N2E2_2358 0.20 -0.8 2,792,468 - Pf6N2E2_2358 0.31 -0.7 2,792,504 - Pf6N2E2_2358 0.35 -1.2 2,792,542 + Pf6N2E2_2358 0.38 +1.0 2,792,543 - Pf6N2E2_2358 0.38 +0.8 2,792,543 - Pf6N2E2_2358 0.38 -0.6 2,792,559 - Pf6N2E2_2358 0.40 +0.3 2,792,630 - Pf6N2E2_2358 0.46 -0.1 2,792,633 + Pf6N2E2_2358 0.46 +0.6 2,792,634 - Pf6N2E2_2358 0.46 -0.2 2,792,664 + Pf6N2E2_2358 0.49 -1.5 2,792,664 + Pf6N2E2_2358 0.49 +0.3 2,792,664 + Pf6N2E2_2358 0.49 -0.4 2,792,664 + Pf6N2E2_2358 0.49 -2.4 2,792,664 + Pf6N2E2_2358 0.49 -0.4 2,792,665 - Pf6N2E2_2358 0.49 -0.8 2,792,665 - Pf6N2E2_2358 0.49 -0.8 2,792,665 - Pf6N2E2_2358 0.49 +0.6 2,792,665 - Pf6N2E2_2358 0.49 -0.2 2,792,665 - Pf6N2E2_2358 0.49 +0.4 2,792,665 - Pf6N2E2_2358 0.49 -1.2 2,792,698 - Pf6N2E2_2358 0.52 +0.2 2,793,126 - +0.3 2,793,126 - +0.2 2,793,204 - +0.3 2,793,410 - Pf6N2E2_2359 0.16 +0.8 2,793,561 + Pf6N2E2_2359 0.29 -0.3 2,793,667 + Pf6N2E2_2359 0.39 +0.8 2,793,667 + Pf6N2E2_2359 0.39 -1.0 2,793,667 + Pf6N2E2_2359 0.39 -0.3 2,793,667 + Pf6N2E2_2359 0.39 +0.3 2,793,668 - Pf6N2E2_2359 0.39 +0.2 2,793,668 - Pf6N2E2_2359 0.39 +1.1 2,793,934 + Pf6N2E2_2359 0.63 +0.5 2,793,934 + Pf6N2E2_2359 0.63 -0.9 2,793,934 + Pf6N2E2_2359 0.63 +0.5 2,794,112 + +0.8 2,794,115 + Pf6N2E2_2359 0.79 -0.6 2,794,115 + Pf6N2E2_2359 0.79 +0.8 2,794,115 + Pf6N2E2_2359 0.79 -0.1 2,794,115 + Pf6N2E2_2359 0.79 +0.5 2,794,116 - Pf6N2E2_2359 0.79 -0.6 2,794,116 - Pf6N2E2_2359 0.79 -0.5
Or see this region's nucleotide sequence