Experiment: Cobalt chloride 0.4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1973 and Pf6N2E2_1974 are separated by 151 nucleotides Pf6N2E2_1974 and Pf6N2E2_1975 overlap by 1 nucleotides Pf6N2E2_1975 and Pf6N2E2_1976 are separated by 11 nucleotides
Pf6N2E2_1973 - FIG141751: hypothetical protein in PFGI-1-like cluster, at 2,339,249 to 2,340,397
_1973
Pf6N2E2_1974 - FIG141694: hypothetical protein in PFGI-1-like cluster, at 2,340,549 to 2,341,340
_1974
Pf6N2E2_1975 - Integrase regulator R, at 2,341,340 to 2,341,840
_1975
Pf6N2E2_1976 - Single-stranded DNA-binding protein, at 2,341,852 to 2,342,256
_1976
Position (kb)
2340
2341
2342 Strain fitness (log2 ratio)
-2
-1
0
1 at 2339.642 kb on + strand, within Pf6N2E2_1973 at 2339.642 kb on + strand, within Pf6N2E2_1973 at 2339.643 kb on - strand, within Pf6N2E2_1973 at 2339.643 kb on - strand, within Pf6N2E2_1973 at 2339.681 kb on - strand, within Pf6N2E2_1973 at 2339.700 kb on - strand, within Pf6N2E2_1973 at 2339.707 kb on - strand, within Pf6N2E2_1973 at 2339.707 kb on - strand, within Pf6N2E2_1973 at 2339.707 kb on - strand, within Pf6N2E2_1973 at 2339.707 kb on - strand, within Pf6N2E2_1973 at 2339.778 kb on + strand, within Pf6N2E2_1973 at 2339.779 kb on - strand, within Pf6N2E2_1973 at 2339.823 kb on + strand, within Pf6N2E2_1973 at 2339.823 kb on + strand, within Pf6N2E2_1973 at 2339.824 kb on - strand, within Pf6N2E2_1973 at 2339.824 kb on - strand, within Pf6N2E2_1973 at 2339.833 kb on - strand, within Pf6N2E2_1973 at 2339.878 kb on + strand, within Pf6N2E2_1973 at 2339.879 kb on - strand, within Pf6N2E2_1973 at 2339.879 kb on - strand, within Pf6N2E2_1973 at 2339.897 kb on - strand, within Pf6N2E2_1973 at 2339.998 kb on + strand, within Pf6N2E2_1973 at 2340.003 kb on + strand, within Pf6N2E2_1973 at 2340.003 kb on + strand, within Pf6N2E2_1973 at 2340.003 kb on + strand, within Pf6N2E2_1973 at 2340.004 kb on - strand, within Pf6N2E2_1973 at 2340.004 kb on - strand, within Pf6N2E2_1973 at 2340.004 kb on - strand, within Pf6N2E2_1973 at 2340.004 kb on - strand, within Pf6N2E2_1973 at 2340.007 kb on + strand, within Pf6N2E2_1973 at 2340.028 kb on - strand, within Pf6N2E2_1973 at 2340.031 kb on + strand, within Pf6N2E2_1973 at 2340.032 kb on - strand, within Pf6N2E2_1973 at 2340.032 kb on - strand, within Pf6N2E2_1973 at 2340.199 kb on - strand, within Pf6N2E2_1973 at 2340.199 kb on - strand, within Pf6N2E2_1973 at 2340.359 kb on + strand at 2340.359 kb on + strand at 2340.360 kb on - strand at 2340.738 kb on + strand, within Pf6N2E2_1974 at 2340.758 kb on - strand, within Pf6N2E2_1974 at 2340.890 kb on - strand, within Pf6N2E2_1974 at 2340.930 kb on + strand, within Pf6N2E2_1974 at 2340.931 kb on - strand, within Pf6N2E2_1974 at 2340.931 kb on - strand, within Pf6N2E2_1974 at 2340.945 kb on + strand, within Pf6N2E2_1974 at 2341.009 kb on + strand, within Pf6N2E2_1974 at 2341.072 kb on - strand, within Pf6N2E2_1974 at 2341.112 kb on + strand, within Pf6N2E2_1974 at 2341.112 kb on + strand, within Pf6N2E2_1974 at 2341.347 kb on + strand at 2341.347 kb on + strand at 2341.348 kb on - strand at 2341.407 kb on + strand, within Pf6N2E2_1975 at 2341.408 kb on - strand, within Pf6N2E2_1975 at 2341.727 kb on - strand, within Pf6N2E2_1975 at 2341.727 kb on - strand, within Pf6N2E2_1975 at 2341.805 kb on + strand at 2341.806 kb on - strand at 2341.836 kb on + strand at 2341.836 kb on + strand at 2341.837 kb on - strand at 2341.837 kb on - strand at 2341.879 kb on - strand at 2341.879 kb on - strand at 2341.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.4 mM remove 2,339,642 + Pf6N2E2_1973 0.34 +1.3 2,339,642 + Pf6N2E2_1973 0.34 -0.3 2,339,643 - Pf6N2E2_1973 0.34 +0.9 2,339,643 - Pf6N2E2_1973 0.34 +0.6 2,339,681 - Pf6N2E2_1973 0.38 -0.5 2,339,700 - Pf6N2E2_1973 0.39 +0.2 2,339,707 - Pf6N2E2_1973 0.40 -0.6 2,339,707 - Pf6N2E2_1973 0.40 -0.0 2,339,707 - Pf6N2E2_1973 0.40 +0.4 2,339,707 - Pf6N2E2_1973 0.40 +0.7 2,339,778 + Pf6N2E2_1973 0.46 -0.6 2,339,779 - Pf6N2E2_1973 0.46 -0.5 2,339,823 + Pf6N2E2_1973 0.50 +1.7 2,339,823 + Pf6N2E2_1973 0.50 -0.6 2,339,824 - Pf6N2E2_1973 0.50 +0.9 2,339,824 - Pf6N2E2_1973 0.50 -0.5 2,339,833 - Pf6N2E2_1973 0.51 -0.1 2,339,878 + Pf6N2E2_1973 0.55 -0.9 2,339,879 - Pf6N2E2_1973 0.55 +0.1 2,339,879 - Pf6N2E2_1973 0.55 -0.3 2,339,897 - Pf6N2E2_1973 0.56 -0.3 2,339,998 + Pf6N2E2_1973 0.65 -1.0 2,340,003 + Pf6N2E2_1973 0.66 +0.3 2,340,003 + Pf6N2E2_1973 0.66 -1.3 2,340,003 + Pf6N2E2_1973 0.66 -0.1 2,340,004 - Pf6N2E2_1973 0.66 -0.2 2,340,004 - Pf6N2E2_1973 0.66 -0.6 2,340,004 - Pf6N2E2_1973 0.66 +0.1 2,340,004 - Pf6N2E2_1973 0.66 -1.3 2,340,007 + Pf6N2E2_1973 0.66 +1.0 2,340,028 - Pf6N2E2_1973 0.68 +1.3 2,340,031 + Pf6N2E2_1973 0.68 -0.8 2,340,032 - Pf6N2E2_1973 0.68 -1.4 2,340,032 - Pf6N2E2_1973 0.68 +0.7 2,340,199 - Pf6N2E2_1973 0.83 -0.3 2,340,199 - Pf6N2E2_1973 0.83 -0.5 2,340,359 + +0.4 2,340,359 + +0.7 2,340,360 - -0.2 2,340,738 + Pf6N2E2_1974 0.24 -0.9 2,340,758 - Pf6N2E2_1974 0.26 -0.3 2,340,890 - Pf6N2E2_1974 0.43 -0.6 2,340,930 + Pf6N2E2_1974 0.48 -2.5 2,340,931 - Pf6N2E2_1974 0.48 -0.3 2,340,931 - Pf6N2E2_1974 0.48 -0.3 2,340,945 + Pf6N2E2_1974 0.50 -0.3 2,341,009 + Pf6N2E2_1974 0.58 -1.1 2,341,072 - Pf6N2E2_1974 0.66 -1.1 2,341,112 + Pf6N2E2_1974 0.71 -0.3 2,341,112 + Pf6N2E2_1974 0.71 +1.1 2,341,347 + +0.2 2,341,347 + +1.1 2,341,348 - +0.1 2,341,407 + Pf6N2E2_1975 0.13 -0.2 2,341,408 - Pf6N2E2_1975 0.14 +0.1 2,341,727 - Pf6N2E2_1975 0.77 +0.4 2,341,727 - Pf6N2E2_1975 0.77 +1.3 2,341,805 + -0.4 2,341,806 - -0.2 2,341,836 + +1.5 2,341,836 + +0.7 2,341,837 - -0.5 2,341,837 - -0.1 2,341,879 - +0.0 2,341,879 - +0.9 2,341,882 - +1.4
Or see this region's nucleotide sequence