Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03195 and HSERO_RS03200 are separated by 194 nucleotides HSERO_RS03200 and HSERO_RS03205 are separated by 93 nucleotides
HSERO_RS03195: HSERO_RS03195 - poly(3-hydroxybutyrate) depolymerase, at 698,712 to 699,938
_RS03195
HSERO_RS03200: HSERO_RS03200 - IMP dehydrogenase, at 700,133 to 703,519
_RS03200
HSERO_RS03205: HSERO_RS03205 - hypothetical protein, at 703,613 to 706,114
_RS03205
Position (kb) 700
701
702
703
704 Strain fitness (log2 ratio) -2
-1
0
1
2 at 699.310 kb on + strand, within HSERO_RS03195 at 699.352 kb on - strand, within HSERO_RS03195 at 699.372 kb on + strand, within HSERO_RS03195 at 699.377 kb on + strand, within HSERO_RS03195 at 699.379 kb on - strand, within HSERO_RS03195 at 699.410 kb on + strand, within HSERO_RS03195 at 699.439 kb on + strand, within HSERO_RS03195 at 699.535 kb on - strand, within HSERO_RS03195 at 699.542 kb on + strand, within HSERO_RS03195 at 699.626 kb on - strand, within HSERO_RS03195 at 699.682 kb on - strand, within HSERO_RS03195 at 699.755 kb on - strand, within HSERO_RS03195 at 699.779 kb on + strand, within HSERO_RS03195 at 699.782 kb on - strand, within HSERO_RS03195 at 699.852 kb on + strand at 700.148 kb on + strand at 700.156 kb on - strand at 700.156 kb on - strand at 700.156 kb on - strand at 700.156 kb on - strand at 700.428 kb on - strand at 700.459 kb on - strand at 700.586 kb on - strand, within HSERO_RS03200 at 700.622 kb on - strand, within HSERO_RS03200 at 700.825 kb on + strand, within HSERO_RS03200 at 700.868 kb on - strand, within HSERO_RS03200 at 700.904 kb on + strand, within HSERO_RS03200 at 700.935 kb on - strand, within HSERO_RS03200 at 701.032 kb on - strand, within HSERO_RS03200 at 701.037 kb on - strand, within HSERO_RS03200 at 701.037 kb on - strand, within HSERO_RS03200 at 701.305 kb on - strand, within HSERO_RS03200 at 701.448 kb on - strand, within HSERO_RS03200 at 701.714 kb on + strand, within HSERO_RS03200 at 701.714 kb on + strand, within HSERO_RS03200 at 701.722 kb on - strand, within HSERO_RS03200 at 701.870 kb on + strand, within HSERO_RS03200 at 701.875 kb on + strand, within HSERO_RS03200 at 701.883 kb on - strand, within HSERO_RS03200 at 701.883 kb on - strand, within HSERO_RS03200 at 701.969 kb on - strand, within HSERO_RS03200 at 702.020 kb on - strand, within HSERO_RS03200 at 702.084 kb on - strand, within HSERO_RS03200 at 702.208 kb on - strand, within HSERO_RS03200 at 702.280 kb on - strand, within HSERO_RS03200 at 702.282 kb on - strand, within HSERO_RS03200 at 702.426 kb on + strand, within HSERO_RS03200 at 702.460 kb on + strand, within HSERO_RS03200 at 702.469 kb on + strand, within HSERO_RS03200 at 702.477 kb on - strand, within HSERO_RS03200 at 702.720 kb on + strand, within HSERO_RS03200 at 702.720 kb on + strand, within HSERO_RS03200 at 702.720 kb on + strand, within HSERO_RS03200 at 702.735 kb on + strand, within HSERO_RS03200 at 702.743 kb on - strand, within HSERO_RS03200 at 702.789 kb on - strand at 703.034 kb on + strand, within HSERO_RS03200 at 703.047 kb on + strand, within HSERO_RS03200 at 703.263 kb on + strand at 703.290 kb on + strand at 703.298 kb on - strand at 703.510 kb on - strand at 703.560 kb on + strand at 703.579 kb on - strand at 703.709 kb on - strand at 703.812 kb on - strand at 703.824 kb on - strand at 703.859 kb on + strand at 703.877 kb on - strand, within HSERO_RS03205 at 704.100 kb on + strand, within HSERO_RS03205 at 704.200 kb on - strand, within HSERO_RS03205 at 704.336 kb on - strand, within HSERO_RS03205 at 704.512 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 699,310 + HSERO_RS03195 0.49 +0.2 699,352 - HSERO_RS03195 0.52 -0.1 699,372 + HSERO_RS03195 0.54 -0.2 699,377 + HSERO_RS03195 0.54 +0.2 699,379 - HSERO_RS03195 0.54 +0.3 699,410 + HSERO_RS03195 0.57 -0.6 699,439 + HSERO_RS03195 0.59 +0.4 699,535 - HSERO_RS03195 0.67 +0.2 699,542 + HSERO_RS03195 0.68 -0.3 699,626 - HSERO_RS03195 0.74 +1.3 699,682 - HSERO_RS03195 0.79 +0.7 699,755 - HSERO_RS03195 0.85 +1.3 699,779 + HSERO_RS03195 0.87 -0.3 699,782 - HSERO_RS03195 0.87 -0.3 699,852 + +0.4 700,148 + +0.2 700,156 - -0.6 700,156 - -2.6 700,156 - -1.1 700,156 - -0.0 700,428 - +0.0 700,459 - -0.0 700,586 - HSERO_RS03200 0.13 +0.0 700,622 - HSERO_RS03200 0.14 +0.1 700,825 + HSERO_RS03200 0.20 -0.1 700,868 - HSERO_RS03200 0.22 -0.3 700,904 + HSERO_RS03200 0.23 +0.6 700,935 - HSERO_RS03200 0.24 +0.3 701,032 - HSERO_RS03200 0.27 +0.0 701,037 - HSERO_RS03200 0.27 -0.4 701,037 - HSERO_RS03200 0.27 +1.3 701,305 - HSERO_RS03200 0.35 +0.3 701,448 - HSERO_RS03200 0.39 -0.0 701,714 + HSERO_RS03200 0.47 -0.9 701,714 + HSERO_RS03200 0.47 +0.0 701,722 - HSERO_RS03200 0.47 +0.0 701,870 + HSERO_RS03200 0.51 +1.4 701,875 + HSERO_RS03200 0.51 -0.5 701,883 - HSERO_RS03200 0.52 -0.4 701,883 - HSERO_RS03200 0.52 +0.3 701,969 - HSERO_RS03200 0.54 -0.2 702,020 - HSERO_RS03200 0.56 +0.1 702,084 - HSERO_RS03200 0.58 -0.2 702,208 - HSERO_RS03200 0.61 +0.5 702,280 - HSERO_RS03200 0.63 -0.3 702,282 - HSERO_RS03200 0.63 +0.1 702,426 + HSERO_RS03200 0.68 -0.4 702,460 + HSERO_RS03200 0.69 +0.2 702,469 + HSERO_RS03200 0.69 -0.4 702,477 - HSERO_RS03200 0.69 -0.2 702,720 + HSERO_RS03200 0.76 -1.2 702,720 + HSERO_RS03200 0.76 +1.0 702,720 + HSERO_RS03200 0.76 +0.2 702,735 + HSERO_RS03200 0.77 +1.8 702,743 - HSERO_RS03200 0.77 +1.3 702,789 - -1.2 703,034 + HSERO_RS03200 0.86 +0.5 703,047 + HSERO_RS03200 0.86 -0.4 703,263 + -0.1 703,290 + +1.0 703,298 - -0.2 703,510 - -0.0 703,560 + -0.2 703,579 - -0.1 703,709 - +0.2 703,812 - +0.3 703,824 - +0.1 703,859 + -0.3 703,877 - HSERO_RS03205 0.11 +0.0 704,100 + HSERO_RS03205 0.19 +0.5 704,200 - HSERO_RS03205 0.23 +0.5 704,336 - HSERO_RS03205 0.29 -0.4 704,512 + +0.6
Or see this region's nucleotide sequence