Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS03185 and HSERO_RS03190 are separated by 75 nucleotides HSERO_RS03190 and HSERO_RS03195 are separated by 266 nucleotides HSERO_RS03195 and HSERO_RS03200 are separated by 194 nucleotides
HSERO_RS03185: HSERO_RS03185 - cupin, at 697,270 to 697,713
_RS03185
HSERO_RS03190: HSERO_RS03190 - glutathione S-transferase, at 697,789 to 698,445
_RS03190
HSERO_RS03195: HSERO_RS03195 - poly(3-hydroxybutyrate) depolymerase, at 698,712 to 699,938
_RS03195
HSERO_RS03200: HSERO_RS03200 - IMP dehydrogenase, at 700,133 to 703,519
_RS03200
Position (kb) 698
699
700 Strain fitness (log2 ratio) -2
-1
0
1
2 at 697.876 kb on - strand, within HSERO_RS03190 at 697.876 kb on - strand, within HSERO_RS03190 at 697.902 kb on + strand, within HSERO_RS03190 at 697.992 kb on - strand, within HSERO_RS03190 at 698.246 kb on - strand, within HSERO_RS03190 at 698.273 kb on - strand, within HSERO_RS03190 at 698.512 kb on - strand at 698.729 kb on + strand at 698.877 kb on + strand at 698.900 kb on + strand, within HSERO_RS03195 at 699.310 kb on + strand, within HSERO_RS03195 at 699.352 kb on - strand, within HSERO_RS03195 at 699.372 kb on + strand, within HSERO_RS03195 at 699.377 kb on + strand, within HSERO_RS03195 at 699.379 kb on - strand, within HSERO_RS03195 at 699.410 kb on + strand, within HSERO_RS03195 at 699.439 kb on + strand, within HSERO_RS03195 at 699.535 kb on - strand, within HSERO_RS03195 at 699.542 kb on + strand, within HSERO_RS03195 at 699.626 kb on - strand, within HSERO_RS03195 at 699.682 kb on - strand, within HSERO_RS03195 at 699.755 kb on - strand, within HSERO_RS03195 at 699.779 kb on + strand, within HSERO_RS03195 at 699.782 kb on - strand, within HSERO_RS03195 at 699.852 kb on + strand at 700.148 kb on + strand at 700.156 kb on - strand at 700.156 kb on - strand at 700.156 kb on - strand at 700.156 kb on - strand at 700.428 kb on - strand at 700.459 kb on - strand at 700.586 kb on - strand, within HSERO_RS03200 at 700.622 kb on - strand, within HSERO_RS03200 at 700.825 kb on + strand, within HSERO_RS03200 at 700.868 kb on - strand, within HSERO_RS03200 at 700.904 kb on + strand, within HSERO_RS03200 at 700.935 kb on - strand, within HSERO_RS03200
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 697,876 - HSERO_RS03190 0.13 +0.4 697,876 - HSERO_RS03190 0.13 -0.2 697,902 + HSERO_RS03190 0.17 +0.0 697,992 - HSERO_RS03190 0.31 -1.6 698,246 - HSERO_RS03190 0.70 -1.0 698,273 - HSERO_RS03190 0.74 -1.3 698,512 - +0.6 698,729 + +0.3 698,877 + -0.9 698,900 + HSERO_RS03195 0.15 +2.0 699,310 + HSERO_RS03195 0.49 +0.2 699,352 - HSERO_RS03195 0.52 -0.1 699,372 + HSERO_RS03195 0.54 -0.2 699,377 + HSERO_RS03195 0.54 +0.2 699,379 - HSERO_RS03195 0.54 +0.3 699,410 + HSERO_RS03195 0.57 -0.6 699,439 + HSERO_RS03195 0.59 +0.4 699,535 - HSERO_RS03195 0.67 +0.2 699,542 + HSERO_RS03195 0.68 -0.3 699,626 - HSERO_RS03195 0.74 +1.3 699,682 - HSERO_RS03195 0.79 +0.7 699,755 - HSERO_RS03195 0.85 +1.3 699,779 + HSERO_RS03195 0.87 -0.3 699,782 - HSERO_RS03195 0.87 -0.3 699,852 + +0.4 700,148 + +0.2 700,156 - -0.6 700,156 - -2.6 700,156 - -1.1 700,156 - -0.0 700,428 - +0.0 700,459 - -0.0 700,586 - HSERO_RS03200 0.13 +0.0 700,622 - HSERO_RS03200 0.14 +0.1 700,825 + HSERO_RS03200 0.20 -0.1 700,868 - HSERO_RS03200 0.22 -0.3 700,904 + HSERO_RS03200 0.23 +0.6 700,935 - HSERO_RS03200 0.24 +0.3
Or see this region's nucleotide sequence