Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20940 and BWI76_RS20945 are separated by 95 nucleotides BWI76_RS20945 and BWI76_RS20950 overlap by 63 nucleotides BWI76_RS20950 and BWI76_RS20955 are separated by 54 nucleotides
BWI76_RS20940: BWI76_RS20940 - uracil/xanthine transporter, at 4,377,577 to 4,378,866
_RS20940
BWI76_RS20945: BWI76_RS20945 - uracil phosphoribosyltransferase, at 4,378,962 to 4,379,588
_RS20945
BWI76_RS20950: BWI76_RS20950 - hypothetical protein, at 4,379,526 to 4,379,716
_RS20950
BWI76_RS20955: BWI76_RS20955 - 6-phospho-beta-glucosidase, at 4,379,771 to 4,381,201
_RS20955
Position (kb) 4378
4379
4380 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4378.272 kb on + strand, within BWI76_RS20940 at 4378.730 kb on + strand, within BWI76_RS20940 at 4378.758 kb on - strand at 4379.050 kb on + strand, within BWI76_RS20945 at 4379.060 kb on - strand, within BWI76_RS20945 at 4379.261 kb on + strand, within BWI76_RS20945 at 4379.477 kb on - strand, within BWI76_RS20945 at 4379.701 kb on + strand at 4379.701 kb on + strand at 4379.709 kb on - strand at 4379.943 kb on - strand, within BWI76_RS20955 at 4380.026 kb on - strand, within BWI76_RS20955 at 4380.122 kb on + strand, within BWI76_RS20955 at 4380.301 kb on - strand, within BWI76_RS20955 at 4380.302 kb on - strand, within BWI76_RS20955 at 4380.324 kb on + strand, within BWI76_RS20955 at 4380.359 kb on + strand, within BWI76_RS20955 at 4380.359 kb on + strand, within BWI76_RS20955 at 4380.367 kb on - strand, within BWI76_RS20955 at 4380.522 kb on - strand, within BWI76_RS20955
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 4,378,272 + BWI76_RS20940 0.54 -1.7 4,378,730 + BWI76_RS20940 0.89 +1.0 4,378,758 - -2.0 4,379,050 + BWI76_RS20945 0.14 -0.9 4,379,060 - BWI76_RS20945 0.16 -0.5 4,379,261 + BWI76_RS20945 0.48 -1.7 4,379,477 - BWI76_RS20945 0.82 +0.1 4,379,701 + -1.0 4,379,701 + +0.4 4,379,709 - +0.4 4,379,943 - BWI76_RS20955 0.12 -0.2 4,380,026 - BWI76_RS20955 0.18 +2.0 4,380,122 + BWI76_RS20955 0.25 +0.9 4,380,301 - BWI76_RS20955 0.37 -0.3 4,380,302 - BWI76_RS20955 0.37 +0.4 4,380,324 + BWI76_RS20955 0.39 +1.7 4,380,359 + BWI76_RS20955 0.41 +0.8 4,380,359 + BWI76_RS20955 0.41 +0.4 4,380,367 - BWI76_RS20955 0.42 +0.4 4,380,522 - BWI76_RS20955 0.52 +0.5
Or see this region's nucleotide sequence