Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19900 and BWI76_RS19905 are separated by 102 nucleotides BWI76_RS19905 and BWI76_RS19910 are separated by 771 nucleotides
BWI76_RS19900: BWI76_RS19900 - FAD:protein FMN transferase, at 4,161,900 to 4,162,952
_RS19900
BWI76_RS19905: BWI76_RS19905 - OmpK36 porin, at 4,163,055 to 4,164,173
_RS19905
BWI76_RS19910: BWI76_RS19910 - phosphotransferase RcsD, at 4,164,945 to 4,167,605
_RS19910
Position (kb) 4163
4164
4165 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 4162.324 kb on + strand, within BWI76_RS19900 at 4162.446 kb on + strand, within BWI76_RS19900 at 4162.783 kb on + strand, within BWI76_RS19900 at 4162.783 kb on + strand, within BWI76_RS19900 at 4162.791 kb on - strand, within BWI76_RS19900 at 4162.793 kb on + strand, within BWI76_RS19900 at 4162.882 kb on - strand at 4162.923 kb on - strand at 4162.983 kb on - strand at 4162.983 kb on - strand at 4163.006 kb on - strand at 4163.761 kb on - strand, within BWI76_RS19905 at 4163.897 kb on + strand, within BWI76_RS19905 at 4163.898 kb on - strand, within BWI76_RS19905 at 4163.968 kb on + strand, within BWI76_RS19905 at 4164.012 kb on - strand, within BWI76_RS19905 at 4164.029 kb on - strand, within BWI76_RS19905 at 4164.029 kb on - strand, within BWI76_RS19905 at 4164.029 kb on - strand, within BWI76_RS19905 at 4164.074 kb on + strand at 4164.074 kb on + strand at 4164.074 kb on + strand at 4164.074 kb on + strand at 4164.082 kb on - strand at 4164.082 kb on - strand at 4164.082 kb on - strand at 4164.112 kb on - strand at 4164.135 kb on - strand at 4164.161 kb on - strand at 4164.218 kb on + strand at 4164.218 kb on + strand at 4164.240 kb on - strand at 4164.433 kb on + strand at 4164.457 kb on + strand at 4164.536 kb on + strand at 4164.541 kb on + strand at 4164.549 kb on - strand at 4164.549 kb on - strand at 4164.603 kb on + strand at 4164.678 kb on + strand at 4164.681 kb on - strand at 4164.725 kb on - strand at 4164.762 kb on + strand at 4164.762 kb on - strand at 4164.769 kb on + strand at 4164.770 kb on - strand at 4164.787 kb on - strand at 4164.787 kb on - strand at 4164.825 kb on - strand at 4164.827 kb on + strand at 4164.833 kb on - strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.855 kb on + strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.863 kb on - strand at 4164.881 kb on - strand at 4164.881 kb on - strand at 4164.881 kb on - strand at 4164.883 kb on + strand at 4164.928 kb on - strand at 4164.930 kb on - strand at 4164.930 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 4,162,324 + BWI76_RS19900 0.40 +0.9 4,162,446 + BWI76_RS19900 0.52 -0.6 4,162,783 + BWI76_RS19900 0.84 -2.0 4,162,783 + BWI76_RS19900 0.84 +1.9 4,162,791 - BWI76_RS19900 0.85 +1.5 4,162,793 + BWI76_RS19900 0.85 -2.2 4,162,882 - -1.1 4,162,923 - -1.2 4,162,983 - +1.2 4,162,983 - +1.7 4,163,006 - -0.6 4,163,761 - BWI76_RS19905 0.63 -2.1 4,163,897 + BWI76_RS19905 0.75 -3.4 4,163,898 - BWI76_RS19905 0.75 -1.1 4,163,968 + BWI76_RS19905 0.82 -3.2 4,164,012 - BWI76_RS19905 0.86 -1.4 4,164,029 - BWI76_RS19905 0.87 -3.1 4,164,029 - BWI76_RS19905 0.87 -2.2 4,164,029 - BWI76_RS19905 0.87 -0.1 4,164,074 + -0.1 4,164,074 + -3.9 4,164,074 + -2.9 4,164,074 + +0.9 4,164,082 - -1.9 4,164,082 - -3.6 4,164,082 - -2.4 4,164,112 - -2.2 4,164,135 - +0.5 4,164,161 - -1.9 4,164,218 + -1.7 4,164,218 + -2.9 4,164,240 - -1.8 4,164,433 + -0.1 4,164,457 + +0.3 4,164,536 + +1.7 4,164,541 + -0.7 4,164,549 - +0.3 4,164,549 - +1.0 4,164,603 + +0.7 4,164,678 + +0.1 4,164,681 - +0.5 4,164,725 - +0.2 4,164,762 + +2.9 4,164,762 - +0.6 4,164,769 + +0.3 4,164,770 - +0.9 4,164,787 - -0.4 4,164,787 - +0.1 4,164,825 - +0.3 4,164,827 + -0.7 4,164,833 - +0.3 4,164,855 + +0.4 4,164,855 + +2.5 4,164,855 + +0.2 4,164,855 + +0.3 4,164,855 + -3.0 4,164,855 + -2.5 4,164,855 + -0.1 4,164,855 + -0.6 4,164,855 + -0.5 4,164,863 - -1.3 4,164,863 - +0.0 4,164,863 - -1.1 4,164,863 - -0.1 4,164,863 - -1.3 4,164,863 - -1.8 4,164,863 - -1.4 4,164,863 - -2.4 4,164,881 - -0.7 4,164,881 - -1.7 4,164,881 - -0.7 4,164,883 + +0.3 4,164,928 - -3.6 4,164,930 - -1.0 4,164,930 - -2.0
Or see this region's nucleotide sequence