Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16960 and BWI76_RS16965 are separated by 311 nucleotides BWI76_RS16965 and BWI76_RS16970 are separated by 64 nucleotides BWI76_RS16970 and BWI76_RS16975 are separated by 101 nucleotides BWI76_RS16975 and BWI76_RS16980 are separated by 11 nucleotides
BWI76_RS16960: BWI76_RS16960 - pyruvate kinase, at 3,517,562 to 3,518,974
_RS16960
BWI76_RS16965: BWI76_RS16965 - murein lipoprotein, at 3,519,286 to 3,519,522
_RS16965
BWI76_RS16970: BWI76_RS16970 - murein L,D-transpeptidase, at 3,519,587 to 3,520,570
_RS16970
BWI76_RS16975: BWI76_RS16975 - cysteine desufuration protein SufE, at 3,520,672 to 3,521,088
_RS16975
BWI76_RS16980: BWI76_RS16980 - bifunctional cysteine desulfurase/selenocysteine lyase, at 3,521,100 to 3,522,320
_RS16980
Position (kb)
3519
3520
3521 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 3519.241 kb on + strand at 3519.249 kb on + strand at 3519.266 kb on - strand at 3519.268 kb on + strand at 3519.276 kb on - strand at 3519.293 kb on + strand at 3519.326 kb on + strand, within BWI76_RS16965 at 3519.481 kb on + strand, within BWI76_RS16965 at 3519.692 kb on - strand, within BWI76_RS16970 at 3519.769 kb on - strand, within BWI76_RS16970 at 3519.776 kb on - strand, within BWI76_RS16970 at 3520.206 kb on + strand, within BWI76_RS16970 at 3520.272 kb on - strand, within BWI76_RS16970 at 3520.441 kb on - strand, within BWI76_RS16970 at 3520.443 kb on + strand, within BWI76_RS16970 at 3520.550 kb on + strand at 3520.648 kb on - strand at 3520.953 kb on + strand, within BWI76_RS16975 at 3521.043 kb on + strand, within BWI76_RS16975 at 3521.051 kb on - strand at 3521.158 kb on + strand at 3521.161 kb on + strand at 3521.169 kb on - strand at 3521.211 kb on - strand at 3521.367 kb on + strand, within BWI76_RS16980 at 3521.390 kb on + strand, within BWI76_RS16980
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,519,241 + -2.5 3,519,249 + -3.0 3,519,266 - -0.8 3,519,268 + -4.2 3,519,276 - -6.5 3,519,293 + -3.1 3,519,326 + BWI76_RS16965 0.17 -4.5 3,519,481 + BWI76_RS16965 0.82 -4.8 3,519,692 - BWI76_RS16970 0.11 +2.0 3,519,769 - BWI76_RS16970 0.18 +2.2 3,519,776 - BWI76_RS16970 0.19 +0.1 3,520,206 + BWI76_RS16970 0.63 -0.0 3,520,272 - BWI76_RS16970 0.70 +0.6 3,520,441 - BWI76_RS16970 0.87 +0.6 3,520,443 + BWI76_RS16970 0.87 +1.1 3,520,550 + +1.1 3,520,648 - +0.5 3,520,953 + BWI76_RS16975 0.67 -0.2 3,521,043 + BWI76_RS16975 0.89 +0.5 3,521,051 - +1.1 3,521,158 + +0.6 3,521,161 + +0.4 3,521,169 - +0.7 3,521,211 - +0.0 3,521,367 + BWI76_RS16980 0.22 -0.1 3,521,390 + BWI76_RS16980 0.24 +0.6
Or see this region's nucleotide sequence