Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15450 and BWI76_RS15455 are separated by 163 nucleotides BWI76_RS15455 and BWI76_RS15460 are separated by 114 nucleotides BWI76_RS15460 and BWI76_RS15465 are separated by 325 nucleotides
BWI76_RS15450: BWI76_RS15450 - hypothetical protein, at 3,237,367 to 3,237,678
_RS15450
BWI76_RS15455: BWI76_RS15455 - nucleoprotein/polynucleotide-associated enzyme, at 3,237,842 to 3,238,381
_RS15455
BWI76_RS15460: BWI76_RS15460 - cold-shock protein, at 3,238,496 to 3,238,972
_RS15460
BWI76_RS15465: BWI76_RS15465 - hypothetical protein, at 3,239,298 to 3,239,909
_RS15465
Position (kb) 3238
3239 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 3237.610 kb on - strand, within BWI76_RS15450 at 3237.781 kb on + strand at 3237.781 kb on + strand at 3237.801 kb on + strand at 3237.809 kb on - strand at 3237.855 kb on + strand at 3237.863 kb on - strand at 3237.911 kb on + strand, within BWI76_RS15455 at 3237.919 kb on - strand, within BWI76_RS15455 at 3237.919 kb on - strand, within BWI76_RS15455 at 3237.919 kb on - strand, within BWI76_RS15455 at 3237.919 kb on - strand, within BWI76_RS15455 at 3237.946 kb on - strand, within BWI76_RS15455 at 3237.947 kb on + strand, within BWI76_RS15455 at 3238.001 kb on + strand, within BWI76_RS15455 at 3238.045 kb on + strand, within BWI76_RS15455 at 3238.053 kb on - strand, within BWI76_RS15455 at 3238.104 kb on + strand, within BWI76_RS15455 at 3238.154 kb on + strand, within BWI76_RS15455 at 3238.172 kb on + strand, within BWI76_RS15455 at 3238.172 kb on + strand, within BWI76_RS15455 at 3238.185 kb on - strand, within BWI76_RS15455 at 3238.295 kb on + strand at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.295 kb on + strand, within BWI76_RS15455 at 3238.303 kb on - strand, within BWI76_RS15455 at 3238.303 kb on - strand, within BWI76_RS15455 at 3238.314 kb on + strand, within BWI76_RS15455 at 3238.327 kb on + strand, within BWI76_RS15455 at 3238.383 kb on - strand at 3238.385 kb on + strand at 3238.393 kb on - strand at 3238.395 kb on + strand at 3238.398 kb on - strand at 3238.403 kb on - strand at 3238.421 kb on - strand at 3238.421 kb on - strand at 3238.428 kb on + strand at 3238.436 kb on + strand at 3238.444 kb on - strand at 3238.444 kb on - strand at 3238.481 kb on - strand at 3238.501 kb on - strand at 3238.507 kb on + strand at 3238.534 kb on - strand at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.596 kb on + strand, within BWI76_RS15460 at 3238.604 kb on - strand, within BWI76_RS15460 at 3238.604 kb on - strand, within BWI76_RS15460 at 3238.606 kb on + strand, within BWI76_RS15460 at 3238.699 kb on + strand, within BWI76_RS15460 at 3238.720 kb on + strand, within BWI76_RS15460 at 3238.720 kb on + strand, within BWI76_RS15460 at 3238.720 kb on + strand, within BWI76_RS15460 at 3238.720 kb on + strand, within BWI76_RS15460 at 3238.725 kb on + strand, within BWI76_RS15460 at 3238.728 kb on - strand, within BWI76_RS15460 at 3238.748 kb on + strand, within BWI76_RS15460 at 3238.758 kb on + strand, within BWI76_RS15460 at 3238.758 kb on + strand, within BWI76_RS15460 at 3238.758 kb on + strand, within BWI76_RS15460 at 3238.787 kb on + strand, within BWI76_RS15460 at 3238.874 kb on + strand, within BWI76_RS15460 at 3238.882 kb on - strand, within BWI76_RS15460 at 3238.885 kb on + strand, within BWI76_RS15460 at 3238.896 kb on + strand, within BWI76_RS15460 at 3238.896 kb on + strand, within BWI76_RS15460 at 3238.896 kb on + strand, within BWI76_RS15460 at 3238.896 kb on + strand, within BWI76_RS15460 at 3238.960 kb on + strand at 3238.960 kb on + strand at 3239.016 kb on - strand at 3239.049 kb on + strand at 3239.068 kb on - strand at 3239.068 kb on - strand at 3239.074 kb on - strand at 3239.074 kb on - strand at 3239.081 kb on - strand at 3239.123 kb on - strand at 3239.123 kb on - strand at 3239.162 kb on - strand at 3239.302 kb on - strand at 3239.329 kb on - strand at 3239.337 kb on + strand at 3239.345 kb on - strand at 3239.345 kb on - strand at 3239.351 kb on - strand at 3239.366 kb on - strand at 3239.366 kb on - strand, within BWI76_RS15465 at 3239.367 kb on - strand, within BWI76_RS15465 at 3239.385 kb on - strand, within BWI76_RS15465 at 3239.425 kb on - strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.483 kb on + strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.491 kb on - strand, within BWI76_RS15465 at 3239.506 kb on - strand, within BWI76_RS15465 at 3239.574 kb on + strand, within BWI76_RS15465 at 3239.574 kb on + strand, within BWI76_RS15465 at 3239.582 kb on - strand, within BWI76_RS15465 at 3239.582 kb on - strand at 3239.582 kb on - strand, within BWI76_RS15465 at 3239.587 kb on + strand, within BWI76_RS15465 at 3239.587 kb on - strand, within BWI76_RS15465 at 3239.587 kb on - strand, within BWI76_RS15465 at 3239.587 kb on - strand, within BWI76_RS15465 at 3239.593 kb on + strand, within BWI76_RS15465 at 3239.595 kb on - strand, within BWI76_RS15465 at 3239.595 kb on - strand at 3239.601 kb on - strand, within BWI76_RS15465 at 3239.601 kb on - strand, within BWI76_RS15465 at 3239.601 kb on - strand, within BWI76_RS15465 at 3239.601 kb on - strand, within BWI76_RS15465 at 3239.657 kb on + strand, within BWI76_RS15465 at 3239.678 kb on - strand at 3239.685 kb on + strand, within BWI76_RS15465 at 3239.697 kb on + strand, within BWI76_RS15465 at 3239.705 kb on - strand, within BWI76_RS15465 at 3239.721 kb on + strand, within BWI76_RS15465 at 3239.726 kb on + strand, within BWI76_RS15465 at 3239.729 kb on - strand, within BWI76_RS15465 at 3239.739 kb on - strand at 3239.755 kb on + strand, within BWI76_RS15465 at 3239.755 kb on + strand, within BWI76_RS15465 at 3239.755 kb on + strand, within BWI76_RS15465 at 3239.755 kb on + strand, within BWI76_RS15465 at 3239.755 kb on + strand, within BWI76_RS15465 at 3239.755 kb on + strand, within BWI76_RS15465 at 3239.755 kb on + strand, within BWI76_RS15465 at 3239.763 kb on - strand, within BWI76_RS15465 at 3239.763 kb on - strand, within BWI76_RS15465 at 3239.763 kb on - strand, within BWI76_RS15465 at 3239.763 kb on - strand at 3239.806 kb on + strand, within BWI76_RS15465 at 3239.806 kb on + strand, within BWI76_RS15465 at 3239.811 kb on + strand, within BWI76_RS15465 at 3239.833 kb on + strand, within BWI76_RS15465 at 3239.833 kb on + strand, within BWI76_RS15465 at 3239.841 kb on - strand, within BWI76_RS15465 at 3239.887 kb on + strand at 3239.920 kb on + strand at 3239.920 kb on + strand at 3239.928 kb on - strand at 3239.928 kb on - strand at 3239.933 kb on + strand at 3239.964 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,237,610 - BWI76_RS15450 0.78 -0.9 3,237,781 + +0.8 3,237,781 + -2.5 3,237,801 + +0.5 3,237,809 - +0.3 3,237,855 + -0.4 3,237,863 - +0.2 3,237,911 + BWI76_RS15455 0.13 -1.4 3,237,919 - BWI76_RS15455 0.14 -1.4 3,237,919 - BWI76_RS15455 0.14 +0.9 3,237,919 - BWI76_RS15455 0.14 -1.3 3,237,919 - BWI76_RS15455 0.14 -1.1 3,237,946 - BWI76_RS15455 0.19 +0.1 3,237,947 + BWI76_RS15455 0.19 +0.3 3,238,001 + BWI76_RS15455 0.29 +0.5 3,238,045 + BWI76_RS15455 0.38 -0.4 3,238,053 - BWI76_RS15455 0.39 -0.3 3,238,104 + BWI76_RS15455 0.49 +0.8 3,238,154 + BWI76_RS15455 0.58 +0.4 3,238,172 + BWI76_RS15455 0.61 -0.4 3,238,172 + BWI76_RS15455 0.61 -1.1 3,238,185 - BWI76_RS15455 0.64 +0.9 3,238,295 + -0.1 3,238,295 + BWI76_RS15455 0.84 +2.1 3,238,295 + BWI76_RS15455 0.84 +1.1 3,238,295 + BWI76_RS15455 0.84 +0.5 3,238,295 + BWI76_RS15455 0.84 -0.2 3,238,295 + BWI76_RS15455 0.84 -0.1 3,238,295 + BWI76_RS15455 0.84 +0.3 3,238,295 + -0.7 3,238,295 + BWI76_RS15455 0.84 +0.2 3,238,295 + BWI76_RS15455 0.84 +0.0 3,238,303 - BWI76_RS15455 0.85 -1.7 3,238,303 - BWI76_RS15455 0.85 -0.8 3,238,314 + BWI76_RS15455 0.87 -1.1 3,238,327 + BWI76_RS15455 0.90 -3.2 3,238,383 - -0.5 3,238,385 + +0.2 3,238,393 - +1.3 3,238,395 + -2.7 3,238,398 - -3.0 3,238,403 - +0.3 3,238,421 - -0.5 3,238,421 - -1.1 3,238,428 + -2.9 3,238,436 + -3.5 3,238,444 - -1.2 3,238,444 - -0.7 3,238,481 - -3.2 3,238,501 - +0.5 3,238,507 + -0.6 3,238,534 - -1.2 3,238,596 + BWI76_RS15460 0.21 -1.1 3,238,596 + BWI76_RS15460 0.21 -2.4 3,238,596 + BWI76_RS15460 0.21 -1.1 3,238,596 + BWI76_RS15460 0.21 +0.2 3,238,596 + BWI76_RS15460 0.21 -1.2 3,238,596 + BWI76_RS15460 0.21 -0.9 3,238,604 - BWI76_RS15460 0.23 -1.0 3,238,604 - BWI76_RS15460 0.23 -1.7 3,238,606 + BWI76_RS15460 0.23 -0.6 3,238,699 + BWI76_RS15460 0.43 -0.1 3,238,720 + BWI76_RS15460 0.47 -0.6 3,238,720 + BWI76_RS15460 0.47 -0.0 3,238,720 + BWI76_RS15460 0.47 -1.7 3,238,720 + BWI76_RS15460 0.47 +0.5 3,238,725 + BWI76_RS15460 0.48 +0.4 3,238,728 - BWI76_RS15460 0.49 -0.1 3,238,748 + BWI76_RS15460 0.53 +0.4 3,238,758 + BWI76_RS15460 0.55 +0.5 3,238,758 + BWI76_RS15460 0.55 +0.7 3,238,758 + BWI76_RS15460 0.55 -1.5 3,238,787 + BWI76_RS15460 0.61 +0.9 3,238,874 + BWI76_RS15460 0.79 -1.2 3,238,882 - BWI76_RS15460 0.81 -0.3 3,238,885 + BWI76_RS15460 0.82 -0.0 3,238,896 + BWI76_RS15460 0.84 -0.4 3,238,896 + BWI76_RS15460 0.84 +0.4 3,238,896 + BWI76_RS15460 0.84 +0.2 3,238,896 + BWI76_RS15460 0.84 -0.6 3,238,960 + +1.7 3,238,960 + +0.6 3,239,016 - +0.1 3,239,049 + +0.9 3,239,068 - -3.4 3,239,068 - -0.9 3,239,074 - +1.8 3,239,074 - -0.2 3,239,081 - +0.3 3,239,123 - +0.2 3,239,123 - +1.8 3,239,162 - +1.9 3,239,302 - +0.3 3,239,329 - +1.1 3,239,337 + -1.2 3,239,345 - -0.7 3,239,345 - -1.8 3,239,351 - -4.0 3,239,366 - -2.5 3,239,366 - BWI76_RS15465 0.11 +2.5 3,239,367 - BWI76_RS15465 0.11 +0.1 3,239,385 - BWI76_RS15465 0.14 -0.1 3,239,425 - BWI76_RS15465 0.21 +0.9 3,239,483 + BWI76_RS15465 0.30 -1.0 3,239,483 + BWI76_RS15465 0.30 -1.0 3,239,483 + +0.9 3,239,483 + BWI76_RS15465 0.30 -0.4 3,239,483 + BWI76_RS15465 0.30 +0.9 3,239,483 + BWI76_RS15465 0.30 -1.5 3,239,483 + BWI76_RS15465 0.30 -0.2 3,239,483 + BWI76_RS15465 0.30 +0.9 3,239,483 + BWI76_RS15465 0.30 +0.9 3,239,483 + BWI76_RS15465 0.30 +0.6 3,239,483 + BWI76_RS15465 0.30 -0.5 3,239,483 + BWI76_RS15465 0.30 -1.9 3,239,483 + BWI76_RS15465 0.30 -1.4 3,239,483 + BWI76_RS15465 0.30 +0.3 3,239,483 + -1.4 3,239,483 + BWI76_RS15465 0.30 -0.2 3,239,483 + BWI76_RS15465 0.30 +0.2 3,239,483 + BWI76_RS15465 0.30 +2.0 3,239,483 + BWI76_RS15465 0.30 -1.1 3,239,491 - BWI76_RS15465 0.32 -0.1 3,239,491 - BWI76_RS15465 0.32 -1.9 3,239,491 - BWI76_RS15465 0.32 +0.4 3,239,491 - BWI76_RS15465 0.32 -0.1 3,239,491 - BWI76_RS15465 0.32 +0.2 3,239,491 - BWI76_RS15465 0.32 -0.7 3,239,491 - -0.1 3,239,491 - BWI76_RS15465 0.32 -0.9 3,239,491 - BWI76_RS15465 0.32 +0.5 3,239,491 - BWI76_RS15465 0.32 -0.7 3,239,491 - BWI76_RS15465 0.32 -2.7 3,239,491 - BWI76_RS15465 0.32 +0.9 3,239,491 - BWI76_RS15465 0.32 -2.1 3,239,491 - BWI76_RS15465 0.32 -0.1 3,239,491 - BWI76_RS15465 0.32 +0.4 3,239,491 - BWI76_RS15465 0.32 -0.7 3,239,491 - BWI76_RS15465 0.32 -2.8 3,239,506 - BWI76_RS15465 0.34 +1.1 3,239,574 + BWI76_RS15465 0.45 +0.9 3,239,574 + BWI76_RS15465 0.45 -0.1 3,239,582 - BWI76_RS15465 0.46 -0.7 3,239,582 - -0.1 3,239,582 - BWI76_RS15465 0.46 -0.7 3,239,587 + BWI76_RS15465 0.47 -1.4 3,239,587 - BWI76_RS15465 0.47 +0.6 3,239,587 - BWI76_RS15465 0.47 -0.9 3,239,587 - BWI76_RS15465 0.47 -0.7 3,239,593 + BWI76_RS15465 0.48 -2.2 3,239,595 - BWI76_RS15465 0.49 +0.3 3,239,595 - -0.1 3,239,601 - BWI76_RS15465 0.50 -0.1 3,239,601 - BWI76_RS15465 0.50 -0.1 3,239,601 - BWI76_RS15465 0.50 +0.5 3,239,601 - BWI76_RS15465 0.50 +0.9 3,239,657 + BWI76_RS15465 0.59 -0.3 3,239,678 - -0.1 3,239,685 + BWI76_RS15465 0.63 -1.9 3,239,697 + BWI76_RS15465 0.65 -0.1 3,239,705 - BWI76_RS15465 0.67 +1.4 3,239,721 + BWI76_RS15465 0.69 -3.1 3,239,726 + BWI76_RS15465 0.70 -0.5 3,239,729 - BWI76_RS15465 0.70 +0.9 3,239,739 - +0.9 3,239,755 + BWI76_RS15465 0.75 -0.8 3,239,755 + BWI76_RS15465 0.75 +0.4 3,239,755 + BWI76_RS15465 0.75 -1.7 3,239,755 + BWI76_RS15465 0.75 -0.2 3,239,755 + BWI76_RS15465 0.75 -2.7 3,239,755 + BWI76_RS15465 0.75 +0.9 3,239,755 + BWI76_RS15465 0.75 -0.2 3,239,763 - BWI76_RS15465 0.76 +0.9 3,239,763 - BWI76_RS15465 0.76 -1.1 3,239,763 - BWI76_RS15465 0.76 -1.1 3,239,763 - -0.7 3,239,806 + BWI76_RS15465 0.83 -3.2 3,239,806 + BWI76_RS15465 0.83 -0.1 3,239,811 + BWI76_RS15465 0.84 -0.9 3,239,833 + BWI76_RS15465 0.87 +0.6 3,239,833 + BWI76_RS15465 0.87 -0.1 3,239,841 - BWI76_RS15465 0.89 -0.4 3,239,887 + -0.2 3,239,920 + -0.7 3,239,920 + +0.6 3,239,928 - +2.3 3,239,928 - -2.5 3,239,933 + +0.5 3,239,964 + -0.7
Or see this region's nucleotide sequence