Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06075 and BWI76_RS06080 overlap by 4 nucleotides BWI76_RS06080 and BWI76_RS06085 are separated by 82 nucleotides BWI76_RS06085 and BWI76_RS06090 are separated by 404 nucleotides
BWI76_RS06075: BWI76_RS06075 - taurine transporter subunit, at 1,276,636 to 1,277,463
_RS06075
BWI76_RS06080: BWI76_RS06080 - taurine dioxygenase, at 1,277,460 to 1,278,311
_RS06080
BWI76_RS06085: BWI76_RS06085 - hypothetical protein, at 1,278,394 to 1,280,112
_RS06085
BWI76_RS06090: BWI76_RS06090 - delta-aminolevulinic acid dehydratase, at 1,280,517 to 1,281,491
_RS06090
Position (kb) 1278
1279
1280
1281 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1277.902 kb on - strand, within BWI76_RS06080 at 1278.216 kb on + strand, within BWI76_RS06080 at 1278.224 kb on - strand, within BWI76_RS06080 at 1278.257 kb on + strand at 1278.460 kb on + strand at 1278.468 kb on - strand at 1278.552 kb on + strand at 1278.554 kb on + strand at 1278.563 kb on + strand at 1278.801 kb on + strand, within BWI76_RS06085 at 1278.847 kb on - strand, within BWI76_RS06085 at 1278.878 kb on - strand, within BWI76_RS06085 at 1278.888 kb on + strand, within BWI76_RS06085 at 1278.896 kb on - strand, within BWI76_RS06085 at 1278.957 kb on + strand, within BWI76_RS06085 at 1279.406 kb on - strand, within BWI76_RS06085 at 1279.511 kb on + strand, within BWI76_RS06085 at 1279.519 kb on - strand, within BWI76_RS06085 at 1279.831 kb on + strand, within BWI76_RS06085 at 1279.831 kb on + strand, within BWI76_RS06085 at 1279.831 kb on + strand, within BWI76_RS06085 at 1279.976 kb on - strand at 1280.071 kb on - strand at 1280.083 kb on - strand at 1280.165 kb on + strand at 1280.371 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 1,277,902 - BWI76_RS06080 0.52 -0.2 1,278,216 + BWI76_RS06080 0.89 +0.1 1,278,224 - BWI76_RS06080 0.90 +0.5 1,278,257 + +1.1 1,278,460 + +0.5 1,278,468 - +0.9 1,278,552 + +0.3 1,278,554 + -2.3 1,278,563 + -1.0 1,278,801 + BWI76_RS06085 0.24 -1.0 1,278,847 - BWI76_RS06085 0.26 -0.6 1,278,878 - BWI76_RS06085 0.28 -0.2 1,278,888 + BWI76_RS06085 0.29 +1.9 1,278,896 - BWI76_RS06085 0.29 -0.4 1,278,957 + BWI76_RS06085 0.33 -0.4 1,279,406 - BWI76_RS06085 0.59 -2.3 1,279,511 + BWI76_RS06085 0.65 -0.0 1,279,519 - BWI76_RS06085 0.65 +0.3 1,279,831 + BWI76_RS06085 0.84 -1.3 1,279,831 + BWI76_RS06085 0.84 -0.2 1,279,831 + BWI76_RS06085 0.84 +0.7 1,279,976 - +1.0 1,280,071 - +0.7 1,280,083 - -2.2 1,280,165 + +0.2 1,280,371 + +0.1
Or see this region's nucleotide sequence