Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04160 and BWI76_RS04165 are separated by 9 nucleotides BWI76_RS04165 and BWI76_RS04170 are separated by 21 nucleotides
BWI76_RS04160: BWI76_RS04160 - phosphoserine phosphatase, at 855,672 to 856,643
_RS04160
BWI76_RS04165: BWI76_RS04165 - DNA repair protein RadA, at 856,653 to 858,035
_RS04165
BWI76_RS04170: BWI76_RS04170 - trifunctional nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase/transcriptional regulator NadR, at 858,057 to 859,289
_RS04170
Position (kb) 856
857
858
859 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 855.691 kb on + strand at 855.726 kb on + strand at 855.797 kb on + strand, within BWI76_RS04160 at 855.866 kb on - strand, within BWI76_RS04160 at 855.919 kb on + strand, within BWI76_RS04160 at 855.927 kb on - strand, within BWI76_RS04160 at 856.200 kb on - strand, within BWI76_RS04160 at 856.310 kb on + strand, within BWI76_RS04160 at 856.331 kb on - strand, within BWI76_RS04160 at 856.571 kb on + strand at 856.593 kb on + strand at 856.598 kb on + strand at 856.603 kb on - strand at 856.606 kb on - strand at 856.617 kb on + strand at 856.695 kb on + strand at 857.087 kb on - strand, within BWI76_RS04165 at 857.376 kb on - strand, within BWI76_RS04165 at 857.473 kb on + strand, within BWI76_RS04165 at 857.652 kb on + strand, within BWI76_RS04165 at 857.682 kb on + strand, within BWI76_RS04165 at 857.999 kb on + strand at 858.067 kb on - strand at 858.314 kb on + strand, within BWI76_RS04170 at 858.324 kb on - strand, within BWI76_RS04170 at 858.530 kb on - strand, within BWI76_RS04170 at 858.594 kb on - strand, within BWI76_RS04170 at 858.772 kb on - strand, within BWI76_RS04170 at 858.893 kb on + strand, within BWI76_RS04170
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 855,691 + +0.4 855,726 + +0.5 855,797 + BWI76_RS04160 0.13 -0.6 855,866 - BWI76_RS04160 0.20 -2.5 855,919 + BWI76_RS04160 0.25 +0.8 855,927 - BWI76_RS04160 0.26 -3.0 856,200 - BWI76_RS04160 0.54 +0.2 856,310 + BWI76_RS04160 0.66 -0.6 856,331 - BWI76_RS04160 0.68 -0.6 856,571 + -2.5 856,593 + -2.2 856,598 + -0.0 856,603 - -0.6 856,606 - -1.8 856,617 + +0.2 856,695 + -0.1 857,087 - BWI76_RS04165 0.31 -0.6 857,376 - BWI76_RS04165 0.52 -1.7 857,473 + BWI76_RS04165 0.59 -2.1 857,652 + BWI76_RS04165 0.72 -0.5 857,682 + BWI76_RS04165 0.74 +0.2 857,999 + -0.4 858,067 - -1.0 858,314 + BWI76_RS04170 0.21 -0.1 858,324 - BWI76_RS04170 0.22 -1.1 858,530 - BWI76_RS04170 0.38 -0.1 858,594 - BWI76_RS04170 0.44 -1.0 858,772 - BWI76_RS04170 0.58 -3.8 858,893 + BWI76_RS04170 0.68 -3.1
Or see this region's nucleotide sequence