Experiment: LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03560 and BWI76_RS03565 overlap by 4 nucleotides BWI76_RS03565 and BWI76_RS03570 are separated by 12 nucleotides BWI76_RS03570 and BWI76_RS03575 are separated by 56 nucleotides
BWI76_RS03560: BWI76_RS03560 - propanediol utilization phosphotransacylase, at 737,398 to 738,018
_RS03560
BWI76_RS03565: BWI76_RS03565 - BMC domain-containing protein, at 738,015 to 738,443
_RS03565
BWI76_RS03570: BWI76_RS03570 - choline TMA-lyase-activating enzyme, at 738,456 to 739,397
_RS03570
BWI76_RS03575: BWI76_RS03575 - choline trimethylamine-lyase, at 739,454 to 742,840
_RS03575
Position (kb) 738
739
740 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 737.746 kb on - strand, within BWI76_RS03560 at 737.830 kb on + strand, within BWI76_RS03560 at 737.830 kb on + strand, within BWI76_RS03560 at 737.838 kb on - strand, within BWI76_RS03560 at 737.838 kb on - strand, within BWI76_RS03560 at 737.890 kb on - strand, within BWI76_RS03560 at 738.014 kb on - strand at 738.029 kb on - strand at 738.092 kb on + strand, within BWI76_RS03565 at 738.179 kb on - strand, within BWI76_RS03565 at 738.179 kb on - strand, within BWI76_RS03565 at 738.209 kb on + strand, within BWI76_RS03565 at 738.229 kb on + strand, within BWI76_RS03565 at 738.292 kb on + strand, within BWI76_RS03565 at 738.357 kb on + strand, within BWI76_RS03565 at 738.393 kb on - strand, within BWI76_RS03565 at 738.513 kb on + strand at 738.513 kb on + strand at 738.513 kb on + strand at 738.513 kb on + strand at 738.521 kb on - strand at 738.553 kb on + strand, within BWI76_RS03570 at 738.553 kb on + strand, within BWI76_RS03570 at 738.561 kb on - strand, within BWI76_RS03570 at 738.561 kb on - strand, within BWI76_RS03570 at 738.592 kb on + strand, within BWI76_RS03570 at 738.596 kb on + strand, within BWI76_RS03570 at 738.604 kb on - strand, within BWI76_RS03570 at 738.676 kb on + strand, within BWI76_RS03570 at 738.697 kb on - strand at 738.744 kb on - strand, within BWI76_RS03570 at 738.748 kb on + strand, within BWI76_RS03570 at 738.757 kb on - strand, within BWI76_RS03570 at 738.757 kb on - strand, within BWI76_RS03570 at 738.801 kb on - strand, within BWI76_RS03570 at 738.846 kb on - strand, within BWI76_RS03570 at 738.846 kb on - strand, within BWI76_RS03570 at 738.875 kb on + strand, within BWI76_RS03570 at 738.880 kb on + strand, within BWI76_RS03570 at 738.886 kb on - strand, within BWI76_RS03570 at 738.904 kb on + strand, within BWI76_RS03570 at 738.904 kb on + strand, within BWI76_RS03570 at 738.909 kb on - strand, within BWI76_RS03570 at 738.912 kb on - strand, within BWI76_RS03570 at 738.912 kb on - strand, within BWI76_RS03570 at 738.912 kb on - strand, within BWI76_RS03570 at 738.912 kb on - strand, within BWI76_RS03570 at 738.945 kb on + strand, within BWI76_RS03570 at 738.962 kb on + strand, within BWI76_RS03570 at 738.962 kb on + strand, within BWI76_RS03570 at 739.028 kb on - strand, within BWI76_RS03570 at 739.043 kb on - strand, within BWI76_RS03570 at 739.066 kb on - strand, within BWI76_RS03570 at 739.098 kb on - strand, within BWI76_RS03570 at 739.098 kb on - strand, within BWI76_RS03570 at 739.098 kb on - strand, within BWI76_RS03570 at 739.099 kb on + strand, within BWI76_RS03570 at 739.113 kb on - strand, within BWI76_RS03570 at 739.195 kb on - strand, within BWI76_RS03570 at 739.199 kb on + strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.233 kb on - strand, within BWI76_RS03570 at 739.233 kb on - strand, within BWI76_RS03570 at 739.246 kb on + strand, within BWI76_RS03570 at 739.267 kb on + strand, within BWI76_RS03570 at 739.268 kb on - strand, within BWI76_RS03570 at 739.275 kb on - strand, within BWI76_RS03570 at 739.275 kb on - strand, within BWI76_RS03570 at 739.275 kb on - strand, within BWI76_RS03570 at 739.275 kb on - strand, within BWI76_RS03570 at 739.275 kb on - strand, within BWI76_RS03570 at 739.417 kb on + strand at 739.421 kb on + strand at 739.421 kb on + strand at 739.426 kb on + strand at 739.426 kb on + strand at 739.426 kb on + strand at 739.429 kb on - strand at 739.434 kb on - strand at 739.434 kb on - strand at 739.450 kb on - strand at 739.500 kb on + strand at 739.500 kb on + strand at 739.500 kb on + strand at 739.503 kb on - strand at 739.508 kb on - strand at 739.508 kb on - strand at 739.508 kb on - strand at 739.510 kb on + strand at 739.510 kb on + strand at 739.510 kb on + strand at 739.510 kb on + strand at 739.518 kb on - strand at 739.518 kb on - strand at 739.518 kb on - strand at 739.518 kb on - strand at 739.518 kb on - strand at 739.524 kb on + strand at 739.534 kb on - strand at 739.652 kb on + strand at 739.669 kb on + strand at 739.706 kb on - strand at 739.812 kb on - strand, within BWI76_RS03575 at 739.860 kb on + strand, within BWI76_RS03575 at 739.918 kb on + strand, within BWI76_RS03575 at 739.926 kb on - strand, within BWI76_RS03575 at 739.931 kb on - strand, within BWI76_RS03575 at 739.940 kb on - strand, within BWI76_RS03575 at 739.968 kb on + strand, within BWI76_RS03575 at 739.995 kb on - strand, within BWI76_RS03575
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 737,746 - BWI76_RS03560 0.56 -0.5 737,830 + BWI76_RS03560 0.70 -0.2 737,830 + BWI76_RS03560 0.70 +0.4 737,838 - BWI76_RS03560 0.71 +1.0 737,838 - BWI76_RS03560 0.71 -0.7 737,890 - BWI76_RS03560 0.79 +0.5 738,014 - +0.2 738,029 - +1.1 738,092 + BWI76_RS03565 0.18 -0.3 738,179 - BWI76_RS03565 0.38 -0.2 738,179 - BWI76_RS03565 0.38 -0.5 738,209 + BWI76_RS03565 0.45 +0.1 738,229 + BWI76_RS03565 0.50 -0.2 738,292 + BWI76_RS03565 0.65 -2.1 738,357 + BWI76_RS03565 0.80 -1.9 738,393 - BWI76_RS03565 0.88 -0.0 738,513 + +0.0 738,513 + +0.2 738,513 + +0.5 738,513 + +0.2 738,521 - -0.7 738,553 + BWI76_RS03570 0.10 +1.0 738,553 + BWI76_RS03570 0.10 +0.1 738,561 - BWI76_RS03570 0.11 -0.4 738,561 - BWI76_RS03570 0.11 +0.6 738,592 + BWI76_RS03570 0.14 +1.5 738,596 + BWI76_RS03570 0.15 +2.1 738,604 - BWI76_RS03570 0.16 +0.2 738,676 + BWI76_RS03570 0.23 -0.1 738,697 - +1.1 738,744 - BWI76_RS03570 0.31 -0.9 738,748 + BWI76_RS03570 0.31 -0.9 738,757 - BWI76_RS03570 0.32 +0.5 738,757 - BWI76_RS03570 0.32 -0.8 738,801 - BWI76_RS03570 0.37 +0.3 738,846 - BWI76_RS03570 0.41 +0.4 738,846 - BWI76_RS03570 0.41 -0.2 738,875 + BWI76_RS03570 0.44 +0.3 738,880 + BWI76_RS03570 0.45 -0.9 738,886 - BWI76_RS03570 0.46 -2.8 738,904 + BWI76_RS03570 0.48 -0.6 738,904 + BWI76_RS03570 0.48 -0.7 738,909 - BWI76_RS03570 0.48 +0.3 738,912 - BWI76_RS03570 0.48 -0.6 738,912 - BWI76_RS03570 0.48 -0.5 738,912 - BWI76_RS03570 0.48 -0.2 738,912 - BWI76_RS03570 0.48 -0.0 738,945 + BWI76_RS03570 0.52 -1.0 738,962 + BWI76_RS03570 0.54 +0.8 738,962 + BWI76_RS03570 0.54 -0.9 739,028 - BWI76_RS03570 0.61 -0.9 739,043 - BWI76_RS03570 0.62 +1.1 739,066 - BWI76_RS03570 0.65 +0.3 739,098 - BWI76_RS03570 0.68 +0.6 739,098 - BWI76_RS03570 0.68 +0.4 739,098 - BWI76_RS03570 0.68 +0.1 739,099 + BWI76_RS03570 0.68 +0.5 739,113 - BWI76_RS03570 0.70 +0.5 739,195 - BWI76_RS03570 0.78 +1.2 739,199 + BWI76_RS03570 0.79 +1.1 739,207 - BWI76_RS03570 0.80 -1.9 739,207 - BWI76_RS03570 0.80 +0.6 739,207 - BWI76_RS03570 0.80 -0.5 739,207 - BWI76_RS03570 0.80 -1.5 739,207 - BWI76_RS03570 0.80 +0.3 739,207 - BWI76_RS03570 0.80 +1.1 739,207 - BWI76_RS03570 0.80 +0.8 739,233 - BWI76_RS03570 0.82 -0.5 739,233 - BWI76_RS03570 0.82 -0.6 739,246 + BWI76_RS03570 0.84 -0.7 739,267 + BWI76_RS03570 0.86 +1.5 739,268 - BWI76_RS03570 0.86 -0.0 739,275 - BWI76_RS03570 0.87 +1.7 739,275 - BWI76_RS03570 0.87 +0.1 739,275 - BWI76_RS03570 0.87 +0.1 739,275 - BWI76_RS03570 0.87 +2.2 739,275 - BWI76_RS03570 0.87 -0.2 739,417 + +0.5 739,421 + +1.1 739,421 + +0.8 739,426 + +1.1 739,426 + -0.2 739,426 + +0.7 739,429 - -0.6 739,434 - +0.7 739,434 - +0.4 739,450 - -0.4 739,500 + -0.4 739,500 + +0.5 739,500 + -0.0 739,503 - -1.2 739,508 - +0.7 739,508 - -0.3 739,508 - +0.0 739,510 + +0.4 739,510 + -0.8 739,510 + -0.0 739,510 + -1.3 739,518 - +0.7 739,518 - -0.8 739,518 - +0.6 739,518 - -1.0 739,518 - +0.5 739,524 + +0.1 739,534 - -0.4 739,652 + -0.4 739,669 + -2.9 739,706 - -0.9 739,812 - BWI76_RS03575 0.11 -3.1 739,860 + BWI76_RS03575 0.12 -0.3 739,918 + BWI76_RS03575 0.14 +0.3 739,926 - BWI76_RS03575 0.14 +0.2 739,931 - BWI76_RS03575 0.14 +0.4 739,940 - BWI76_RS03575 0.14 -1.8 739,968 + BWI76_RS03575 0.15 -2.5 739,995 - BWI76_RS03575 0.16 +0.1
Or see this region's nucleotide sequence