Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_677 and Pf6N2E2_678 are separated by 120 nucleotides Pf6N2E2_678 and Pf6N2E2_679 overlap by 4 nucleotides Pf6N2E2_679 and Pf6N2E2_680 are separated by 191 nucleotides
Pf6N2E2_677 - Transcriptional regulator, AraC family, at 782,169 to 782,918
_677
Pf6N2E2_678 - Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster, at 783,039 to 784,295
_678
Pf6N2E2_679 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 784,292 to 784,528
_679
Pf6N2E2_680 - D-amino-acid oxidase (EC 1.4.3.3), at 784,720 to 785,835
_680
Position (kb) 783
784
785 Strain fitness (log2 ratio) -2
-1
0
1
2 at 782.060 kb on + strand at 782.060 kb on + strand at 782.121 kb on - strand at 782.252 kb on - strand, within Pf6N2E2_677 at 782.456 kb on + strand, within Pf6N2E2_677 at 782.457 kb on - strand, within Pf6N2E2_677 at 782.592 kb on + strand, within Pf6N2E2_677 at 782.592 kb on + strand, within Pf6N2E2_677 at 782.592 kb on + strand, within Pf6N2E2_677 at 782.593 kb on - strand, within Pf6N2E2_677 at 782.593 kb on - strand, within Pf6N2E2_677 at 782.611 kb on + strand, within Pf6N2E2_677 at 782.611 kb on + strand, within Pf6N2E2_677 at 782.914 kb on + strand at 782.914 kb on + strand at 782.914 kb on + strand at 782.914 kb on + strand at 782.914 kb on + strand at 782.914 kb on + strand at 782.915 kb on - strand at 782.915 kb on - strand at 782.915 kb on - strand at 782.915 kb on - strand at 782.915 kb on - strand at 782.915 kb on - strand at 783.240 kb on + strand, within Pf6N2E2_678 at 783.241 kb on - strand, within Pf6N2E2_678 at 783.466 kb on + strand, within Pf6N2E2_678 at 783.466 kb on + strand, within Pf6N2E2_678 at 783.546 kb on + strand, within Pf6N2E2_678 at 783.546 kb on + strand, within Pf6N2E2_678 at 783.622 kb on + strand, within Pf6N2E2_678 at 783.623 kb on - strand, within Pf6N2E2_678 at 784.000 kb on + strand, within Pf6N2E2_678 at 784.001 kb on - strand, within Pf6N2E2_678 at 784.047 kb on - strand, within Pf6N2E2_678 at 784.089 kb on + strand, within Pf6N2E2_678 at 784.089 kb on + strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.387 kb on + strand, within Pf6N2E2_679 at 784.387 kb on + strand, within Pf6N2E2_679 at 784.517 kb on + strand at 784.518 kb on - strand at 784.760 kb on + strand at 784.760 kb on + strand at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 782,060 + +1.3 782,060 + +0.3 782,121 - +0.5 782,252 - Pf6N2E2_677 0.11 -0.1 782,456 + Pf6N2E2_677 0.38 +0.2 782,457 - Pf6N2E2_677 0.38 +1.1 782,592 + Pf6N2E2_677 0.56 +1.0 782,592 + Pf6N2E2_677 0.56 -1.0 782,592 + Pf6N2E2_677 0.56 -0.7 782,593 - Pf6N2E2_677 0.57 -0.2 782,593 - Pf6N2E2_677 0.57 +0.2 782,611 + Pf6N2E2_677 0.59 -1.1 782,611 + Pf6N2E2_677 0.59 -0.1 782,914 + -0.1 782,914 + +0.5 782,914 + +2.7 782,914 + +0.1 782,914 + +0.4 782,914 + -1.2 782,915 - -0.6 782,915 - -0.0 782,915 - +0.5 782,915 - -1.5 782,915 - -0.8 782,915 - +0.3 783,240 + Pf6N2E2_678 0.16 -1.5 783,241 - Pf6N2E2_678 0.16 -0.5 783,466 + Pf6N2E2_678 0.34 -1.7 783,466 + Pf6N2E2_678 0.34 -1.2 783,546 + Pf6N2E2_678 0.40 -0.9 783,546 + Pf6N2E2_678 0.40 +0.3 783,622 + Pf6N2E2_678 0.46 +0.1 783,623 - Pf6N2E2_678 0.46 -0.3 784,000 + Pf6N2E2_678 0.76 -0.3 784,001 - Pf6N2E2_678 0.77 +0.2 784,047 - Pf6N2E2_678 0.80 -1.3 784,089 + Pf6N2E2_678 0.84 +1.1 784,089 + Pf6N2E2_678 0.84 -0.7 784,090 - Pf6N2E2_678 0.84 -0.9 784,090 - Pf6N2E2_678 0.84 -1.3 784,090 - Pf6N2E2_678 0.84 -0.3 784,090 - Pf6N2E2_678 0.84 -0.1 784,387 + Pf6N2E2_679 0.40 -1.9 784,387 + Pf6N2E2_679 0.40 -1.5 784,517 + -0.4 784,518 - -1.3 784,760 + -0.1 784,760 + -1.7 785,092 + Pf6N2E2_680 0.33 -0.6 785,092 + Pf6N2E2_680 0.33 +1.2 785,092 + Pf6N2E2_680 0.33 +1.5 785,092 + Pf6N2E2_680 0.33 +1.1 785,092 + Pf6N2E2_680 0.33 -1.5 785,093 - Pf6N2E2_680 0.33 -0.9 785,093 - Pf6N2E2_680 0.33 +1.0 785,093 - Pf6N2E2_680 0.33 -0.2 785,093 - Pf6N2E2_680 0.33 -0.9 785,093 - Pf6N2E2_680 0.33 +1.7 785,093 - Pf6N2E2_680 0.33 +0.4 785,093 - Pf6N2E2_680 0.33 +2.7 785,094 + Pf6N2E2_680 0.34 +0.1 785,094 + Pf6N2E2_680 0.34 +0.9 785,095 - Pf6N2E2_680 0.34 +0.7 785,095 - Pf6N2E2_680 0.34 +0.3 785,142 - Pf6N2E2_680 0.38 -0.5 785,142 - Pf6N2E2_680 0.38 -0.3
Or see this region's nucleotide sequence