Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5074 and Pf6N2E2_5075 are separated by 115 nucleotides Pf6N2E2_5075 and Pf6N2E2_5076 are separated by 43 nucleotides Pf6N2E2_5076 and Pf6N2E2_5077 are separated by 192 nucleotides Pf6N2E2_5077 and Pf6N2E2_5078 are separated by 157 nucleotides
Pf6N2E2_5074 - Catalase (EC 1.11.1.6), at 5,849,985 to 5,851,526
_5074
Pf6N2E2_5075 - FOG: Ankyrin repeat, at 5,851,642 to 5,852,193
_5075
Pf6N2E2_5076 - DNA repair protein RadA, at 5,852,237 to 5,853,604
_5076
Pf6N2E2_5077 - hypothetical protein, at 5,853,797 to 5,854,153
_5077
Pf6N2E2_5078 - Carbon starvation protein A, at 5,854,311 to 5,856,377
_5078
Position (kb) 5852
5853
5854 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5851.245 kb on + strand, within Pf6N2E2_5074 at 5851.245 kb on + strand, within Pf6N2E2_5074 at 5851.245 kb on + strand, within Pf6N2E2_5074 at 5851.246 kb on - strand, within Pf6N2E2_5074 at 5851.326 kb on + strand, within Pf6N2E2_5074 at 5851.432 kb on - strand at 5851.432 kb on - strand at 5851.450 kb on - strand at 5851.625 kb on - strand at 5851.625 kb on - strand at 5851.661 kb on + strand at 5851.691 kb on + strand at 5851.691 kb on + strand at 5851.730 kb on + strand, within Pf6N2E2_5075 at 5851.731 kb on - strand, within Pf6N2E2_5075 at 5851.750 kb on + strand, within Pf6N2E2_5075 at 5851.750 kb on + strand, within Pf6N2E2_5075 at 5851.753 kb on + strand, within Pf6N2E2_5075 at 5851.753 kb on + strand, within Pf6N2E2_5075 at 5851.754 kb on - strand, within Pf6N2E2_5075 at 5851.800 kb on + strand, within Pf6N2E2_5075 at 5851.801 kb on - strand, within Pf6N2E2_5075 at 5851.813 kb on + strand, within Pf6N2E2_5075 at 5851.843 kb on + strand, within Pf6N2E2_5075 at 5851.843 kb on + strand, within Pf6N2E2_5075 at 5851.868 kb on - strand, within Pf6N2E2_5075 at 5851.868 kb on - strand, within Pf6N2E2_5075 at 5851.868 kb on - strand, within Pf6N2E2_5075 at 5851.868 kb on - strand, within Pf6N2E2_5075 at 5851.942 kb on - strand, within Pf6N2E2_5075 at 5851.942 kb on - strand, within Pf6N2E2_5075 at 5852.056 kb on + strand, within Pf6N2E2_5075 at 5852.056 kb on + strand, within Pf6N2E2_5075 at 5852.056 kb on + strand, within Pf6N2E2_5075 at 5852.056 kb on + strand, within Pf6N2E2_5075 at 5852.056 kb on + strand, within Pf6N2E2_5075 at 5852.056 kb on - strand, within Pf6N2E2_5075 at 5852.057 kb on - strand, within Pf6N2E2_5075 at 5852.057 kb on - strand, within Pf6N2E2_5075 at 5852.057 kb on - strand, within Pf6N2E2_5075 at 5852.057 kb on - strand, within Pf6N2E2_5075 at 5852.057 kb on - strand, within Pf6N2E2_5075 at 5852.057 kb on - strand, within Pf6N2E2_5075 at 5852.057 kb on - strand, within Pf6N2E2_5075 at 5852.057 kb on - strand, within Pf6N2E2_5075 at 5852.258 kb on + strand at 5852.259 kb on - strand at 5852.259 kb on - strand at 5852.259 kb on - strand at 5852.259 kb on - strand at 5852.502 kb on + strand, within Pf6N2E2_5076 at 5852.503 kb on - strand, within Pf6N2E2_5076 at 5852.603 kb on + strand, within Pf6N2E2_5076 at 5852.604 kb on - strand, within Pf6N2E2_5076 at 5852.604 kb on - strand, within Pf6N2E2_5076 at 5852.604 kb on - strand, within Pf6N2E2_5076 at 5852.877 kb on + strand, within Pf6N2E2_5076 at 5852.878 kb on - strand, within Pf6N2E2_5076 at 5852.948 kb on + strand, within Pf6N2E2_5076 at 5852.948 kb on + strand, within Pf6N2E2_5076 at 5853.327 kb on + strand, within Pf6N2E2_5076 at 5853.328 kb on - strand, within Pf6N2E2_5076 at 5853.608 kb on + strand at 5853.645 kb on - strand at 5853.645 kb on - strand at 5853.645 kb on - strand at 5853.645 kb on - strand at 5853.645 kb on - strand at 5853.663 kb on + strand at 5853.663 kb on + strand at 5853.664 kb on - strand at 5853.690 kb on + strand at 5853.690 kb on + strand at 5853.690 kb on + strand at 5853.690 kb on + strand at 5853.691 kb on - strand at 5853.697 kb on + strand at 5853.764 kb on + strand at 5853.765 kb on - strand at 5853.765 kb on - strand at 5853.873 kb on - strand, within Pf6N2E2_5077 at 5853.873 kb on - strand, within Pf6N2E2_5077 at 5853.873 kb on - strand, within Pf6N2E2_5077 at 5853.873 kb on - strand, within Pf6N2E2_5077 at 5853.985 kb on + strand, within Pf6N2E2_5077 at 5854.131 kb on - strand at 5854.131 kb on - strand at 5854.154 kb on + strand at 5854.182 kb on + strand at 5854.182 kb on + strand at 5854.183 kb on - strand at 5854.184 kb on + strand at 5854.184 kb on + strand at 5854.184 kb on + strand at 5854.184 kb on + strand at 5854.184 kb on + strand at 5854.184 kb on + strand at 5854.184 kb on + strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.185 kb on - strand at 5854.305 kb on - strand at 5854.306 kb on + strand at 5854.306 kb on + strand at 5854.322 kb on + strand at 5854.322 kb on + strand at 5854.322 kb on + strand at 5854.323 kb on - strand at 5854.323 kb on - strand at 5854.323 kb on - strand at 5854.323 kb on - strand at 5854.323 kb on - strand at 5854.323 kb on - strand at 5854.323 kb on - strand at 5854.325 kb on + strand at 5854.326 kb on - strand at 5854.326 kb on - strand at 5854.326 kb on - strand at 5854.326 kb on - strand at 5854.342 kb on + strand at 5854.342 kb on + strand at 5854.343 kb on - strand at 5854.343 kb on - strand at 5854.343 kb on - strand at 5854.352 kb on - strand at 5854.366 kb on + strand at 5854.367 kb on - strand at 5854.424 kb on - strand at 5854.453 kb on - strand at 5854.453 kb on - strand at 5854.453 kb on - strand at 5854.471 kb on - strand at 5854.474 kb on - strand at 5854.474 kb on - strand at 5854.474 kb on - strand at 5854.474 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 5,851,245 + Pf6N2E2_5074 0.82 -1.5 5,851,245 + Pf6N2E2_5074 0.82 +0.1 5,851,245 + Pf6N2E2_5074 0.82 +0.4 5,851,246 - Pf6N2E2_5074 0.82 +2.2 5,851,326 + Pf6N2E2_5074 0.87 -0.6 5,851,432 - -0.3 5,851,432 - +0.5 5,851,450 - -0.1 5,851,625 - -0.7 5,851,625 - -0.7 5,851,661 + +0.6 5,851,691 + +0.5 5,851,691 + +0.0 5,851,730 + Pf6N2E2_5075 0.16 -0.4 5,851,731 - Pf6N2E2_5075 0.16 +0.0 5,851,750 + Pf6N2E2_5075 0.20 +0.2 5,851,750 + Pf6N2E2_5075 0.20 -0.4 5,851,753 + Pf6N2E2_5075 0.20 +0.1 5,851,753 + Pf6N2E2_5075 0.20 +0.9 5,851,754 - Pf6N2E2_5075 0.20 +1.2 5,851,800 + Pf6N2E2_5075 0.29 +0.2 5,851,801 - Pf6N2E2_5075 0.29 -1.6 5,851,813 + Pf6N2E2_5075 0.31 -0.2 5,851,843 + Pf6N2E2_5075 0.36 -0.0 5,851,843 + Pf6N2E2_5075 0.36 +0.7 5,851,868 - Pf6N2E2_5075 0.41 +0.3 5,851,868 - Pf6N2E2_5075 0.41 +1.6 5,851,868 - Pf6N2E2_5075 0.41 +0.5 5,851,868 - Pf6N2E2_5075 0.41 +0.7 5,851,942 - Pf6N2E2_5075 0.54 +0.5 5,851,942 - Pf6N2E2_5075 0.54 +1.2 5,852,056 + Pf6N2E2_5075 0.75 +0.7 5,852,056 + Pf6N2E2_5075 0.75 +1.0 5,852,056 + Pf6N2E2_5075 0.75 +0.4 5,852,056 + Pf6N2E2_5075 0.75 +0.7 5,852,056 + Pf6N2E2_5075 0.75 +0.2 5,852,056 - Pf6N2E2_5075 0.75 -1.6 5,852,057 - Pf6N2E2_5075 0.75 -0.3 5,852,057 - Pf6N2E2_5075 0.75 -2.7 5,852,057 - Pf6N2E2_5075 0.75 +1.6 5,852,057 - Pf6N2E2_5075 0.75 -0.4 5,852,057 - Pf6N2E2_5075 0.75 -0.2 5,852,057 - Pf6N2E2_5075 0.75 -0.1 5,852,057 - Pf6N2E2_5075 0.75 -0.6 5,852,057 - Pf6N2E2_5075 0.75 +0.4 5,852,258 + -0.4 5,852,259 - -0.3 5,852,259 - -0.2 5,852,259 - +0.4 5,852,259 - +1.5 5,852,502 + Pf6N2E2_5076 0.19 -0.4 5,852,503 - Pf6N2E2_5076 0.19 -0.2 5,852,603 + Pf6N2E2_5076 0.27 -0.2 5,852,604 - Pf6N2E2_5076 0.27 -0.8 5,852,604 - Pf6N2E2_5076 0.27 -1.6 5,852,604 - Pf6N2E2_5076 0.27 +1.8 5,852,877 + Pf6N2E2_5076 0.47 -0.3 5,852,878 - Pf6N2E2_5076 0.47 -0.2 5,852,948 + Pf6N2E2_5076 0.52 -1.4 5,852,948 + Pf6N2E2_5076 0.52 -0.4 5,853,327 + Pf6N2E2_5076 0.80 -1.1 5,853,328 - Pf6N2E2_5076 0.80 -1.5 5,853,608 + +0.9 5,853,645 - -0.1 5,853,645 - +0.1 5,853,645 - +2.2 5,853,645 - +1.0 5,853,645 - -0.0 5,853,663 + +2.0 5,853,663 + -0.4 5,853,664 - +0.5 5,853,690 + -0.5 5,853,690 + +0.3 5,853,690 + -0.5 5,853,690 + -0.8 5,853,691 - +0.3 5,853,697 + -0.1 5,853,764 + +0.2 5,853,765 - -1.4 5,853,765 - -0.5 5,853,873 - Pf6N2E2_5077 0.21 -0.9 5,853,873 - Pf6N2E2_5077 0.21 +0.1 5,853,873 - Pf6N2E2_5077 0.21 +1.0 5,853,873 - Pf6N2E2_5077 0.21 -2.3 5,853,985 + Pf6N2E2_5077 0.53 -0.6 5,854,131 - +0.0 5,854,131 - -0.5 5,854,154 + -0.6 5,854,182 + +0.7 5,854,182 + +0.4 5,854,183 - -0.6 5,854,184 + +1.9 5,854,184 + +1.2 5,854,184 + -0.2 5,854,184 + -0.4 5,854,184 + +1.0 5,854,184 + +1.0 5,854,184 + +0.9 5,854,185 - -0.3 5,854,185 - +0.4 5,854,185 - +0.5 5,854,185 - -1.1 5,854,185 - +0.3 5,854,185 - -0.3 5,854,185 - +1.2 5,854,185 - +0.5 5,854,185 - -0.4 5,854,185 - +0.2 5,854,185 - +0.2 5,854,185 - -0.7 5,854,305 - +0.3 5,854,306 + +1.6 5,854,306 + -0.4 5,854,322 + +0.1 5,854,322 + -0.6 5,854,322 + -0.5 5,854,323 - -0.1 5,854,323 - -0.5 5,854,323 - +0.5 5,854,323 - -1.0 5,854,323 - +1.1 5,854,323 - +0.4 5,854,323 - -0.5 5,854,325 + -0.8 5,854,326 - +0.2 5,854,326 - -0.4 5,854,326 - +0.3 5,854,326 - +0.1 5,854,342 + +0.3 5,854,342 + +0.1 5,854,343 - -0.6 5,854,343 - +0.6 5,854,343 - -0.4 5,854,352 - +0.3 5,854,366 + +0.9 5,854,367 - +0.4 5,854,424 - -0.3 5,854,453 - +0.1 5,854,453 - -0.1 5,854,453 - -1.0 5,854,471 - +0.7 5,854,474 - -0.7 5,854,474 - -0.2 5,854,474 - -0.2 5,854,474 - +0.7
Or see this region's nucleotide sequence