Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1850 and Pf6N2E2_1851 are separated by 63 nucleotides Pf6N2E2_1851 and Pf6N2E2_1852 are separated by 389 nucleotides
Pf6N2E2_1850 - Enoyl-CoA hydratase [isoleucine degradation] (EC 4.2.1.17) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,189,848 to 2,191,995
_1850
Pf6N2E2_1851 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 2,192,059 to 2,193,267
_1851
Pf6N2E2_1852 - Regulatory protein, LuxR:Tetratricopeptide TPR_4, at 2,193,657 to 2,196,332
_1852
Position (kb)
2192
2193
2194 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2191.364 kb on - strand, within Pf6N2E2_1850 at 2191.635 kb on + strand, within Pf6N2E2_1850 at 2191.636 kb on - strand, within Pf6N2E2_1850 at 2191.870 kb on + strand at 2191.905 kb on - strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.995 kb on + strand at 2191.996 kb on - strand at 2192.451 kb on + strand, within Pf6N2E2_1851 at 2192.505 kb on + strand, within Pf6N2E2_1851 at 2192.547 kb on - strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.550 kb on + strand, within Pf6N2E2_1851 at 2192.551 kb on - strand, within Pf6N2E2_1851 at 2192.560 kb on + strand, within Pf6N2E2_1851 at 2192.561 kb on - strand, within Pf6N2E2_1851 at 2192.571 kb on - strand, within Pf6N2E2_1851 at 2192.581 kb on - strand, within Pf6N2E2_1851 at 2193.011 kb on - strand, within Pf6N2E2_1851 at 2193.127 kb on - strand, within Pf6N2E2_1851 at 2193.137 kb on + strand, within Pf6N2E2_1851 at 2193.194 kb on + strand at 2193.246 kb on - strand at 2193.246 kb on - strand at 2193.254 kb on - strand at 2193.310 kb on + strand at 2193.311 kb on - strand at 2193.311 kb on - strand at 2193.376 kb on - strand at 2193.376 kb on - strand at 2193.471 kb on + strand at 2193.504 kb on + strand at 2193.504 kb on + strand at 2193.504 kb on + strand at 2193.505 kb on - strand at 2193.553 kb on + strand at 2193.599 kb on + strand at 2193.600 kb on - strand at 2193.600 kb on - strand at 2193.604 kb on + strand at 2193.604 kb on + strand at 2193.605 kb on - strand at 2193.685 kb on - strand at 2193.949 kb on + strand, within Pf6N2E2_1852 at 2193.950 kb on - strand, within Pf6N2E2_1852 at 2194.142 kb on - strand, within Pf6N2E2_1852 at 2194.142 kb on - strand, within Pf6N2E2_1852 at 2194.142 kb on - strand, within Pf6N2E2_1852 at 2194.148 kb on - strand, within Pf6N2E2_1852 at 2194.148 kb on - strand, within Pf6N2E2_1852 at 2194.148 kb on - strand, within Pf6N2E2_1852 at 2194.171 kb on + strand, within Pf6N2E2_1852 at 2194.171 kb on + strand, within Pf6N2E2_1852 at 2194.171 kb on + strand, within Pf6N2E2_1852 at 2194.172 kb on - strand, within Pf6N2E2_1852
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 2,191,364 - Pf6N2E2_1850 0.71 +0.9 2,191,635 + Pf6N2E2_1850 0.83 +0.1 2,191,636 - Pf6N2E2_1850 0.83 -0.1 2,191,870 + +1.7 2,191,905 - +0.4 2,191,995 + +0.7 2,191,995 + -0.3 2,191,995 + +1.8 2,191,995 + -0.0 2,191,995 + -0.7 2,191,996 - +0.1 2,192,451 + Pf6N2E2_1851 0.32 +0.3 2,192,505 + Pf6N2E2_1851 0.37 +0.4 2,192,547 - Pf6N2E2_1851 0.40 +0.1 2,192,550 + Pf6N2E2_1851 0.41 +1.6 2,192,550 + Pf6N2E2_1851 0.41 +3.3 2,192,550 + Pf6N2E2_1851 0.41 +0.1 2,192,551 - Pf6N2E2_1851 0.41 -2.2 2,192,560 + Pf6N2E2_1851 0.41 -0.9 2,192,561 - Pf6N2E2_1851 0.42 +0.7 2,192,571 - Pf6N2E2_1851 0.42 +1.7 2,192,581 - Pf6N2E2_1851 0.43 -0.3 2,193,011 - Pf6N2E2_1851 0.79 +0.7 2,193,127 - Pf6N2E2_1851 0.88 +0.5 2,193,137 + Pf6N2E2_1851 0.89 +0.2 2,193,194 + -0.1 2,193,246 - +0.0 2,193,246 - +0.1 2,193,254 - -2.1 2,193,310 + -0.1 2,193,311 - +2.3 2,193,311 - +1.1 2,193,376 - -0.6 2,193,376 - +0.6 2,193,471 + +0.1 2,193,504 + +0.5 2,193,504 + +0.1 2,193,504 + -0.3 2,193,505 - +0.2 2,193,553 + -0.4 2,193,599 + -2.6 2,193,600 - -0.9 2,193,600 - +0.7 2,193,604 + -0.1 2,193,604 + -0.5 2,193,605 - +2.0 2,193,685 - +0.7 2,193,949 + Pf6N2E2_1852 0.11 -1.6 2,193,950 - Pf6N2E2_1852 0.11 -0.7 2,194,142 - Pf6N2E2_1852 0.18 -0.1 2,194,142 - Pf6N2E2_1852 0.18 +0.5 2,194,142 - Pf6N2E2_1852 0.18 -0.5 2,194,148 - Pf6N2E2_1852 0.18 +0.7 2,194,148 - Pf6N2E2_1852 0.18 -0.0 2,194,148 - Pf6N2E2_1852 0.18 -0.6 2,194,171 + Pf6N2E2_1852 0.19 -0.1 2,194,171 + Pf6N2E2_1852 0.19 +1.0 2,194,171 + Pf6N2E2_1852 0.19 -0.5 2,194,172 - Pf6N2E2_1852 0.19 +1.1
Or see this region's nucleotide sequence