Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_161 and Pf6N2E2_162 are separated by 58 nucleotides Pf6N2E2_162 and Pf6N2E2_163 overlap by 4 nucleotides Pf6N2E2_163 and Pf6N2E2_164 are separated by 3 nucleotides
Pf6N2E2_161 - Ribose ABC transport system, periplasmic ribose-binding protein RbsB (TC 3.A.1.2.1), at 214,281 to 215,237
_161
Pf6N2E2_162 - Ribose ABC transport system, ATP-binding protein RbsA (TC 3.A.1.2.1), at 215,296 to 216,849
_162
Pf6N2E2_163 - Ribose ABC transport system, permease protein RbsC (TC 3.A.1.2.1), at 216,846 to 217,823
_163
Pf6N2E2_164 - Ribose operon repressor, at 217,827 to 218,846
_164
Position (kb) 215
216
217 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.455 kb on + strand, within Pf6N2E2_161 at 214.456 kb on - strand, within Pf6N2E2_161 at 214.753 kb on - strand, within Pf6N2E2_161 at 214.753 kb on - strand, within Pf6N2E2_161 at 215.235 kb on + strand at 215.235 kb on + strand at 215.235 kb on + strand at 215.236 kb on - strand at 215.236 kb on - strand at 215.236 kb on - strand at 215.236 kb on - strand at 215.240 kb on - strand at 215.240 kb on - strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.295 kb on + strand at 215.296 kb on - strand at 215.350 kb on + strand at 215.350 kb on + strand at 215.351 kb on - strand at 215.351 kb on - strand at 215.351 kb on - strand at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.893 kb on + strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.894 kb on - strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.989 kb on + strand, within Pf6N2E2_162 at 215.990 kb on - strand, within Pf6N2E2_162 at 215.990 kb on - strand, within Pf6N2E2_162 at 215.990 kb on - strand, within Pf6N2E2_162 at 216.087 kb on + strand, within Pf6N2E2_162 at 216.156 kb on + strand, within Pf6N2E2_162 at 216.616 kb on + strand, within Pf6N2E2_162 at 216.616 kb on + strand, within Pf6N2E2_162 at 216.616 kb on + strand, within Pf6N2E2_162 at 216.616 kb on + strand, within Pf6N2E2_162 at 216.617 kb on - strand, within Pf6N2E2_162 at 216.617 kb on - strand, within Pf6N2E2_162 at 216.617 kb on - strand, within Pf6N2E2_162 at 216.617 kb on - strand, within Pf6N2E2_162 at 216.888 kb on + strand at 216.889 kb on - strand at 216.903 kb on + strand at 216.904 kb on - strand at 216.975 kb on + strand, within Pf6N2E2_163 at 216.975 kb on + strand, within Pf6N2E2_163 at 216.990 kb on - strand, within Pf6N2E2_163 at 217.302 kb on + strand, within Pf6N2E2_163 at 217.302 kb on + strand, within Pf6N2E2_163 at 217.303 kb on - strand, within Pf6N2E2_163 at 217.303 kb on - strand, within Pf6N2E2_163 at 217.492 kb on - strand, within Pf6N2E2_163 at 217.505 kb on - strand, within Pf6N2E2_163 at 217.631 kb on + strand, within Pf6N2E2_163 at 217.631 kb on + strand, within Pf6N2E2_163 at 217.791 kb on + strand at 217.792 kb on - strand at 217.847 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 214,455 + Pf6N2E2_161 0.18 +1.0 214,455 + Pf6N2E2_161 0.18 +2.2 214,455 + Pf6N2E2_161 0.18 +2.7 214,456 - Pf6N2E2_161 0.18 +1.0 214,753 - Pf6N2E2_161 0.49 +1.1 214,753 - Pf6N2E2_161 0.49 +2.7 215,235 + +0.9 215,235 + +1.5 215,235 + +2.7 215,236 - +2.0 215,236 - +2.1 215,236 - +1.2 215,236 - +1.4 215,240 - +2.3 215,240 - +0.8 215,295 + +1.3 215,295 + +0.8 215,295 + +1.5 215,295 + +2.7 215,295 + +2.3 215,295 + +1.0 215,295 + +1.7 215,295 + +1.5 215,295 + +1.4 215,296 - +1.5 215,350 + +0.8 215,350 + +2.0 215,351 - +1.4 215,351 - +1.2 215,351 - +2.2 215,893 + Pf6N2E2_162 0.38 +1.6 215,893 + Pf6N2E2_162 0.38 +2.9 215,893 + Pf6N2E2_162 0.38 +1.9 215,893 + Pf6N2E2_162 0.38 +1.3 215,893 + Pf6N2E2_162 0.38 +1.3 215,893 + Pf6N2E2_162 0.38 +1.9 215,894 - Pf6N2E2_162 0.38 +2.0 215,894 - Pf6N2E2_162 0.38 +1.7 215,894 - Pf6N2E2_162 0.38 -1.2 215,894 - Pf6N2E2_162 0.38 +1.3 215,894 - Pf6N2E2_162 0.38 +1.9 215,989 + Pf6N2E2_162 0.45 +0.8 215,989 + Pf6N2E2_162 0.45 +1.8 215,989 + Pf6N2E2_162 0.45 +2.2 215,989 + Pf6N2E2_162 0.45 +1.0 215,990 - Pf6N2E2_162 0.45 +4.1 215,990 - Pf6N2E2_162 0.45 +1.6 215,990 - Pf6N2E2_162 0.45 +1.2 216,087 + Pf6N2E2_162 0.51 -0.5 216,156 + Pf6N2E2_162 0.55 +3.0 216,616 + Pf6N2E2_162 0.85 +1.2 216,616 + Pf6N2E2_162 0.85 +1.5 216,616 + Pf6N2E2_162 0.85 +2.8 216,616 + Pf6N2E2_162 0.85 +1.9 216,617 - Pf6N2E2_162 0.85 +1.1 216,617 - Pf6N2E2_162 0.85 +1.9 216,617 - Pf6N2E2_162 0.85 +1.5 216,617 - Pf6N2E2_162 0.85 +1.9 216,888 + +0.4 216,889 - +0.9 216,903 + +3.0 216,904 - +2.7 216,975 + Pf6N2E2_163 0.13 +1.4 216,975 + Pf6N2E2_163 0.13 +1.7 216,990 - Pf6N2E2_163 0.15 +2.3 217,302 + Pf6N2E2_163 0.47 +1.5 217,302 + Pf6N2E2_163 0.47 -0.2 217,303 - Pf6N2E2_163 0.47 +2.9 217,303 - Pf6N2E2_163 0.47 +2.5 217,492 - Pf6N2E2_163 0.66 +2.2 217,505 - Pf6N2E2_163 0.67 +2.6 217,631 + Pf6N2E2_163 0.80 +1.8 217,631 + Pf6N2E2_163 0.80 +1.8 217,791 + +1.6 217,792 - +1.9 217,847 - +1.0
Or see this region's nucleotide sequence