Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1562 and Pf6N2E2_1563 are separated by 93 nucleotides Pf6N2E2_1563 and Pf6N2E2_1564 are separated by 242 nucleotides
Pf6N2E2_1562 - Threonine dehydratase (EC 4.3.1.19), at 1,823,631 to 1,824,587
_1562
Pf6N2E2_1563 - Transcriptional regulator, AraC family, at 1,824,681 to 1,825,496
_1563
Pf6N2E2_1564 - 3-dehydroquinate synthase (EC 4.2.3.4), at 1,825,739 to 1,827,613
_1564
Position (kb)
1824
1825
1826 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1823.782 kb on + strand, within Pf6N2E2_1562 at 1823.782 kb on + strand, within Pf6N2E2_1562 at 1823.782 kb on + strand, within Pf6N2E2_1562 at 1823.783 kb on - strand, within Pf6N2E2_1562 at 1823.783 kb on - strand, within Pf6N2E2_1562 at 1823.785 kb on + strand, within Pf6N2E2_1562 at 1823.786 kb on - strand, within Pf6N2E2_1562 at 1823.826 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.851 kb on + strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.852 kb on - strand, within Pf6N2E2_1562 at 1823.907 kb on - strand, within Pf6N2E2_1562 at 1823.907 kb on - strand, within Pf6N2E2_1562 at 1824.064 kb on + strand, within Pf6N2E2_1562 at 1824.064 kb on + strand, within Pf6N2E2_1562 at 1824.065 kb on - strand, within Pf6N2E2_1562 at 1824.065 kb on - strand, within Pf6N2E2_1562 at 1824.110 kb on - strand, within Pf6N2E2_1562 at 1824.276 kb on - strand, within Pf6N2E2_1562 at 1824.511 kb on + strand at 1824.538 kb on + strand at 1824.538 kb on + strand at 1824.539 kb on - strand at 1824.539 kb on - strand at 1824.539 kb on - strand at 1824.541 kb on - strand at 1824.541 kb on - strand at 1824.616 kb on - strand at 1824.678 kb on - strand at 1824.678 kb on - strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.680 kb on + strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.681 kb on - strand at 1824.799 kb on - strand, within Pf6N2E2_1563 at 1824.960 kb on + strand, within Pf6N2E2_1563 at 1824.960 kb on + strand, within Pf6N2E2_1563 at 1824.960 kb on + strand, within Pf6N2E2_1563 at 1825.160 kb on - strand, within Pf6N2E2_1563 at 1825.160 kb on - strand, within Pf6N2E2_1563 at 1825.282 kb on + strand, within Pf6N2E2_1563 at 1825.596 kb on - strand at 1825.661 kb on - strand at 1825.697 kb on + strand at 1825.697 kb on + strand at 1825.719 kb on - strand at 1825.719 kb on - strand at 1825.919 kb on + strand at 1826.298 kb on - strand, within Pf6N2E2_1564 at 1826.308 kb on - strand, within Pf6N2E2_1564 at 1826.364 kb on + strand, within Pf6N2E2_1564 at 1826.459 kb on + strand, within Pf6N2E2_1564 at 1826.460 kb on - strand, within Pf6N2E2_1564 at 1826.460 kb on - strand, within Pf6N2E2_1564 at 1826.490 kb on + strand at 1826.491 kb on - strand, within Pf6N2E2_1564 at 1826.491 kb on - strand, within Pf6N2E2_1564 at 1826.491 kb on - strand, within Pf6N2E2_1564 at 1826.491 kb on - strand, within Pf6N2E2_1564 at 1826.491 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 1,823,782 + Pf6N2E2_1562 0.16 +0.6 1,823,782 + Pf6N2E2_1562 0.16 -0.4 1,823,782 + Pf6N2E2_1562 0.16 +1.4 1,823,783 - Pf6N2E2_1562 0.16 +2.2 1,823,783 - Pf6N2E2_1562 0.16 +1.2 1,823,785 + Pf6N2E2_1562 0.16 -1.0 1,823,786 - Pf6N2E2_1562 0.16 +1.2 1,823,826 + Pf6N2E2_1562 0.20 +0.1 1,823,851 + Pf6N2E2_1562 0.23 +0.2 1,823,851 + Pf6N2E2_1562 0.23 +0.4 1,823,851 + Pf6N2E2_1562 0.23 +1.4 1,823,851 + Pf6N2E2_1562 0.23 +1.0 1,823,851 + Pf6N2E2_1562 0.23 -0.2 1,823,851 + Pf6N2E2_1562 0.23 +0.0 1,823,852 - Pf6N2E2_1562 0.23 +0.0 1,823,852 - Pf6N2E2_1562 0.23 -0.2 1,823,852 - Pf6N2E2_1562 0.23 -0.8 1,823,852 - Pf6N2E2_1562 0.23 +0.5 1,823,852 - Pf6N2E2_1562 0.23 +0.6 1,823,907 - Pf6N2E2_1562 0.29 -0.4 1,823,907 - Pf6N2E2_1562 0.29 +1.0 1,824,064 + Pf6N2E2_1562 0.45 -1.0 1,824,064 + Pf6N2E2_1562 0.45 -2.0 1,824,065 - Pf6N2E2_1562 0.45 +1.1 1,824,065 - Pf6N2E2_1562 0.45 +0.1 1,824,110 - Pf6N2E2_1562 0.50 +0.2 1,824,276 - Pf6N2E2_1562 0.67 +0.6 1,824,511 + +1.9 1,824,538 + +0.9 1,824,538 + -0.7 1,824,539 - -0.7 1,824,539 - +0.0 1,824,539 - -0.5 1,824,541 - +0.1 1,824,541 - +0.8 1,824,616 - +1.6 1,824,678 - +0.6 1,824,678 - +0.9 1,824,680 + -2.0 1,824,680 + -0.8 1,824,680 + +0.6 1,824,680 + +0.0 1,824,680 + +0.5 1,824,680 + -0.8 1,824,680 + -0.1 1,824,681 - +1.5 1,824,681 - +0.6 1,824,681 - +0.2 1,824,681 - -0.8 1,824,681 - -0.2 1,824,681 - -0.2 1,824,681 - +0.6 1,824,681 - +0.8 1,824,681 - +0.6 1,824,681 - +1.0 1,824,681 - +0.6 1,824,681 - -0.7 1,824,681 - +0.6 1,824,681 - -0.1 1,824,799 - Pf6N2E2_1563 0.14 -0.4 1,824,960 + Pf6N2E2_1563 0.34 -1.2 1,824,960 + Pf6N2E2_1563 0.34 -1.3 1,824,960 + Pf6N2E2_1563 0.34 +0.2 1,825,160 - Pf6N2E2_1563 0.59 -0.5 1,825,160 - Pf6N2E2_1563 0.59 -0.4 1,825,282 + Pf6N2E2_1563 0.74 -0.7 1,825,596 - +0.5 1,825,661 - -0.3 1,825,697 + -0.4 1,825,697 + +0.9 1,825,719 - +1.2 1,825,719 - -0.2 1,825,919 + +1.6 1,826,298 - Pf6N2E2_1564 0.30 -0.7 1,826,308 - Pf6N2E2_1564 0.30 -0.4 1,826,364 + Pf6N2E2_1564 0.33 +0.6 1,826,459 + Pf6N2E2_1564 0.38 -1.0 1,826,460 - Pf6N2E2_1564 0.38 -2.2 1,826,460 - Pf6N2E2_1564 0.38 +0.6 1,826,490 + -0.4 1,826,491 - Pf6N2E2_1564 0.40 -0.4 1,826,491 - Pf6N2E2_1564 0.40 -1.0 1,826,491 - Pf6N2E2_1564 0.40 +0.2 1,826,491 - Pf6N2E2_1564 0.40 +0.7 1,826,491 - -0.4
Or see this region's nucleotide sequence