Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_140 and Pf6N2E2_141 are separated by 903 nucleotides Pf6N2E2_141 and Pf6N2E2_142 are separated by 192 nucleotides
Pf6N2E2_140 - Glutamate carboxypeptidase (EC 3.4.17.11), at 190,063 to 191,301
_140
Pf6N2E2_141 - Phosphate ABC transporter, periplasmic phosphate-binding protein PstS (TC 3.A.1.7.1), at 192,205 to 193,539
_141
Pf6N2E2_142 - Exodeoxyribonuclease III (EC 3.1.11.2), at 193,732 to 194,544
_142
Position (kb) 192
193
194 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 191.227 kb on + strand at 191.227 kb on + strand at 191.228 kb on - strand at 191.260 kb on + strand at 191.307 kb on + strand at 191.307 kb on + strand at 191.308 kb on - strand at 191.458 kb on - strand at 191.464 kb on - strand at 191.468 kb on - strand at 191.468 kb on - strand at 191.468 kb on - strand at 191.817 kb on + strand at 191.817 kb on + strand at 191.818 kb on - strand at 191.818 kb on - strand at 191.818 kb on - strand at 191.958 kb on - strand at 191.958 kb on - strand at 191.958 kb on - strand at 191.964 kb on - strand at 192.317 kb on + strand at 192.449 kb on + strand, within Pf6N2E2_141 at 192.449 kb on + strand, within Pf6N2E2_141 at 192.450 kb on - strand, within Pf6N2E2_141 at 192.450 kb on - strand, within Pf6N2E2_141 at 192.450 kb on - strand, within Pf6N2E2_141 at 192.714 kb on - strand, within Pf6N2E2_141 at 192.827 kb on + strand, within Pf6N2E2_141 at 192.960 kb on + strand, within Pf6N2E2_141 at 192.960 kb on + strand, within Pf6N2E2_141 at 192.960 kb on + strand, within Pf6N2E2_141 at 192.960 kb on + strand, within Pf6N2E2_141 at 192.960 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.965 kb on + strand, within Pf6N2E2_141 at 192.966 kb on - strand, within Pf6N2E2_141 at 192.966 kb on - strand, within Pf6N2E2_141 at 192.966 kb on - strand, within Pf6N2E2_141 at 192.966 kb on - strand, within Pf6N2E2_141 at 192.992 kb on + strand, within Pf6N2E2_141 at 192.992 kb on + strand, within Pf6N2E2_141 at 193.111 kb on - strand, within Pf6N2E2_141 at 193.428 kb on + strand at 193.448 kb on - strand at 193.577 kb on + strand at 193.577 kb on + strand at 193.620 kb on + strand at 193.628 kb on + strand at 193.628 kb on + strand at 193.629 kb on - strand at 193.629 kb on - strand at 194.216 kb on + strand, within Pf6N2E2_142 at 194.216 kb on + strand, within Pf6N2E2_142 at 194.315 kb on + strand, within Pf6N2E2_142 at 194.315 kb on + strand, within Pf6N2E2_142 at 194.316 kb on - strand, within Pf6N2E2_142 at 194.316 kb on - strand, within Pf6N2E2_142 at 194.316 kb on - strand, within Pf6N2E2_142 at 194.316 kb on - strand, within Pf6N2E2_142 at 194.378 kb on + strand, within Pf6N2E2_142
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 191,227 + -0.1 191,227 + +0.8 191,228 - -0.5 191,260 + +0.7 191,307 + +0.7 191,307 + -0.4 191,308 - -1.3 191,458 - +0.3 191,464 - +0.2 191,468 - +0.6 191,468 - -0.8 191,468 - -1.0 191,817 + -0.2 191,817 + +0.0 191,818 - -0.5 191,818 - +0.8 191,818 - -0.1 191,958 - +0.7 191,958 - +0.5 191,958 - -0.3 191,964 - +1.1 192,317 + -3.4 192,449 + Pf6N2E2_141 0.18 -0.5 192,449 + Pf6N2E2_141 0.18 -0.5 192,450 - Pf6N2E2_141 0.18 +0.2 192,450 - Pf6N2E2_141 0.18 +1.9 192,450 - Pf6N2E2_141 0.18 -0.3 192,714 - Pf6N2E2_141 0.38 +0.8 192,827 + Pf6N2E2_141 0.47 -0.2 192,960 + Pf6N2E2_141 0.57 +0.5 192,960 + Pf6N2E2_141 0.57 -0.8 192,960 + Pf6N2E2_141 0.57 +0.5 192,960 + Pf6N2E2_141 0.57 -0.9 192,960 + Pf6N2E2_141 0.57 -0.5 192,965 + Pf6N2E2_141 0.57 +0.2 192,965 + Pf6N2E2_141 0.57 +0.8 192,965 + Pf6N2E2_141 0.57 -0.0 192,965 + Pf6N2E2_141 0.57 +0.1 192,965 + Pf6N2E2_141 0.57 -0.6 192,965 + Pf6N2E2_141 0.57 -0.3 192,965 + Pf6N2E2_141 0.57 +0.7 192,966 - Pf6N2E2_141 0.57 -0.4 192,966 - Pf6N2E2_141 0.57 +0.2 192,966 - Pf6N2E2_141 0.57 -0.5 192,966 - Pf6N2E2_141 0.57 -0.8 192,992 + Pf6N2E2_141 0.59 +0.2 192,992 + Pf6N2E2_141 0.59 +0.0 193,111 - Pf6N2E2_141 0.68 -1.5 193,428 + +0.0 193,448 - -0.4 193,577 + +1.8 193,577 + -0.8 193,620 + -1.6 193,628 + +0.8 193,628 + -0.8 193,629 - +0.8 193,629 - -0.4 194,216 + Pf6N2E2_142 0.60 -1.0 194,216 + Pf6N2E2_142 0.60 -1.5 194,315 + Pf6N2E2_142 0.72 -0.8 194,315 + Pf6N2E2_142 0.72 -0.3 194,316 - Pf6N2E2_142 0.72 +0.8 194,316 - Pf6N2E2_142 0.72 +0.3 194,316 - Pf6N2E2_142 0.72 +0.5 194,316 - Pf6N2E2_142 0.72 +0.2 194,378 + Pf6N2E2_142 0.79 +1.1
Or see this region's nucleotide sequence