Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1273 and Pf6N2E2_1274 are separated by 192 nucleotides Pf6N2E2_1274 and Pf6N2E2_1275 are separated by 127 nucleotides Pf6N2E2_1275 and Pf6N2E2_1276 are separated by 92 nucleotides Pf6N2E2_1276 and Pf6N2E2_1277 are separated by 99 nucleotides
Pf6N2E2_1273 - Probable RND efflux membrane fusion protein, at 1,504,055 to 1,505,356
_1273
Pf6N2E2_1274 - Thiol peroxidase, Tpx-type (EC 1.11.1.15), at 1,505,549 to 1,506,049
_1274
Pf6N2E2_1275 - Putative outer membrane lipoprotein, at 1,506,177 to 1,506,848
_1275
Pf6N2E2_1276 - FIG00958406: hypothetical protein, at 1,506,941 to 1,507,435
_1276
Pf6N2E2_1277 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 1,507,535 to 1,508,032
_1277
Position (kb)
1506
1507 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1505.300 kb on + strand at 1505.300 kb on + strand at 1505.301 kb on - strand at 1505.451 kb on + strand at 1505.452 kb on - strand at 1505.463 kb on - strand at 1505.538 kb on + strand at 1505.545 kb on + strand at 1505.546 kb on - strand at 1505.546 kb on - strand at 1505.577 kb on + strand at 1505.578 kb on - strand at 1505.700 kb on + strand, within Pf6N2E2_1274 at 1506.119 kb on + strand at 1506.120 kb on - strand at 1506.120 kb on - strand at 1506.132 kb on - strand at 1506.436 kb on + strand, within Pf6N2E2_1275 at 1506.437 kb on - strand, within Pf6N2E2_1275 at 1506.496 kb on - strand, within Pf6N2E2_1275 at 1506.496 kb on - strand, within Pf6N2E2_1275 at 1506.496 kb on - strand, within Pf6N2E2_1275 at 1506.562 kb on + strand, within Pf6N2E2_1275 at 1506.562 kb on + strand, within Pf6N2E2_1275 at 1506.562 kb on + strand, within Pf6N2E2_1275 at 1506.565 kb on + strand, within Pf6N2E2_1275 at 1506.565 kb on + strand, within Pf6N2E2_1275 at 1506.565 kb on + strand, within Pf6N2E2_1275 at 1506.646 kb on + strand, within Pf6N2E2_1275 at 1506.646 kb on + strand, within Pf6N2E2_1275 at 1506.647 kb on - strand, within Pf6N2E2_1275 at 1506.647 kb on - strand, within Pf6N2E2_1275 at 1506.647 kb on - strand, within Pf6N2E2_1275 at 1506.658 kb on + strand, within Pf6N2E2_1275 at 1506.659 kb on - strand, within Pf6N2E2_1275 at 1506.740 kb on - strand, within Pf6N2E2_1275 at 1506.740 kb on - strand, within Pf6N2E2_1275 at 1506.740 kb on - strand, within Pf6N2E2_1275 at 1506.761 kb on - strand, within Pf6N2E2_1275 at 1506.889 kb on - strand at 1506.889 kb on - strand at 1506.894 kb on + strand at 1506.894 kb on + strand at 1506.895 kb on - strand at 1506.895 kb on - strand at 1506.895 kb on - strand at 1506.897 kb on - strand at 1507.082 kb on - strand, within Pf6N2E2_1276 at 1507.136 kb on + strand, within Pf6N2E2_1276 at 1507.136 kb on + strand, within Pf6N2E2_1276 at 1507.137 kb on - strand, within Pf6N2E2_1276 at 1507.137 kb on - strand, within Pf6N2E2_1276 at 1507.248 kb on + strand, within Pf6N2E2_1276 at 1507.248 kb on + strand, within Pf6N2E2_1276 at 1507.248 kb on + strand, within Pf6N2E2_1276 at 1507.249 kb on - strand, within Pf6N2E2_1276 at 1507.249 kb on - strand, within Pf6N2E2_1276 at 1507.317 kb on + strand, within Pf6N2E2_1276 at 1507.318 kb on - strand, within Pf6N2E2_1276 at 1507.318 kb on - strand, within Pf6N2E2_1276 at 1507.424 kb on + strand at 1507.503 kb on + strand at 1507.503 kb on + strand at 1507.504 kb on - strand at 1507.504 kb on - strand at 1507.591 kb on - strand, within Pf6N2E2_1277
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 1,505,300 + +0.5 1,505,300 + +1.2 1,505,301 - -0.2 1,505,451 + +1.5 1,505,452 - -1.5 1,505,463 - -1.0 1,505,538 + +0.1 1,505,545 + +0.5 1,505,546 - -0.2 1,505,546 - -0.5 1,505,577 + +1.2 1,505,578 - -0.7 1,505,700 + Pf6N2E2_1274 0.30 +0.6 1,506,119 + -0.3 1,506,120 - -0.2 1,506,120 - +0.6 1,506,132 - -0.4 1,506,436 + Pf6N2E2_1275 0.39 -1.3 1,506,437 - Pf6N2E2_1275 0.39 -0.3 1,506,496 - Pf6N2E2_1275 0.47 +1.6 1,506,496 - Pf6N2E2_1275 0.47 +0.6 1,506,496 - Pf6N2E2_1275 0.47 +0.6 1,506,562 + Pf6N2E2_1275 0.57 -2.8 1,506,562 + Pf6N2E2_1275 0.57 -0.4 1,506,562 + Pf6N2E2_1275 0.57 -0.8 1,506,565 + Pf6N2E2_1275 0.58 -1.8 1,506,565 + Pf6N2E2_1275 0.58 -0.4 1,506,565 + Pf6N2E2_1275 0.58 -0.2 1,506,646 + Pf6N2E2_1275 0.70 -0.4 1,506,646 + Pf6N2E2_1275 0.70 -1.9 1,506,647 - Pf6N2E2_1275 0.70 -1.0 1,506,647 - Pf6N2E2_1275 0.70 -0.8 1,506,647 - Pf6N2E2_1275 0.70 -0.6 1,506,658 + Pf6N2E2_1275 0.72 -1.8 1,506,659 - Pf6N2E2_1275 0.72 +0.6 1,506,740 - Pf6N2E2_1275 0.84 +1.0 1,506,740 - Pf6N2E2_1275 0.84 -0.9 1,506,740 - Pf6N2E2_1275 0.84 -2.0 1,506,761 - Pf6N2E2_1275 0.87 -0.6 1,506,889 - -0.7 1,506,889 - +1.3 1,506,894 + +0.6 1,506,894 + -0.4 1,506,895 - -0.3 1,506,895 - +0.6 1,506,895 - +0.6 1,506,897 - -0.4 1,507,082 - Pf6N2E2_1276 0.28 +0.3 1,507,136 + Pf6N2E2_1276 0.39 +2.6 1,507,136 + Pf6N2E2_1276 0.39 +0.6 1,507,137 - Pf6N2E2_1276 0.40 -0.0 1,507,137 - Pf6N2E2_1276 0.40 +0.4 1,507,248 + Pf6N2E2_1276 0.62 +0.6 1,507,248 + Pf6N2E2_1276 0.62 +1.9 1,507,248 + Pf6N2E2_1276 0.62 -1.0 1,507,249 - Pf6N2E2_1276 0.62 +2.1 1,507,249 - Pf6N2E2_1276 0.62 -0.4 1,507,317 + Pf6N2E2_1276 0.76 +0.1 1,507,318 - Pf6N2E2_1276 0.76 +0.6 1,507,318 - Pf6N2E2_1276 0.76 -0.1 1,507,424 + +1.1 1,507,503 + +0.6 1,507,503 + -0.4 1,507,504 - -0.8 1,507,504 - -1.3 1,507,591 - Pf6N2E2_1277 0.11 -0.4
Or see this region's nucleotide sequence