Experiment: Nickel (II) chloride 1.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_111 and Pf6N2E2_112 are separated by 5 nucleotides Pf6N2E2_112 and Pf6N2E2_113 overlap by 4 nucleotides Pf6N2E2_113 and Pf6N2E2_114 are separated by 104 nucleotides
Pf6N2E2_111 - FIG00954746: hypothetical protein, at 156,445 to 157,620
_111
Pf6N2E2_112 - hypothetical protein, at 157,626 to 158,315
_112
Pf6N2E2_113 - FIG00963512: hypothetical protein, at 158,312 to 160,225
_113
Pf6N2E2_114 - Polysaccharide biosynthesis protein, at 160,330 to 161,634
_114
Position (kb) 158
159
160
161 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.462 kb on + strand, within Pf6N2E2_111 at 157.463 kb on - strand, within Pf6N2E2_111 at 157.559 kb on + strand at 157.664 kb on + strand at 157.665 kb on - strand at 157.694 kb on - strand at 157.736 kb on - strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.776 kb on + strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.777 kb on - strand, within Pf6N2E2_112 at 157.778 kb on + strand, within Pf6N2E2_112 at 157.778 kb on + strand, within Pf6N2E2_112 at 157.779 kb on - strand, within Pf6N2E2_112 at 157.806 kb on + strand, within Pf6N2E2_112 at 157.806 kb on + strand, within Pf6N2E2_112 at 157.806 kb on + strand, within Pf6N2E2_112 at 157.807 kb on - strand, within Pf6N2E2_112 at 157.934 kb on - strand, within Pf6N2E2_112 at 157.984 kb on + strand, within Pf6N2E2_112 at 158.038 kb on + strand, within Pf6N2E2_112 at 158.038 kb on + strand, within Pf6N2E2_112 at 158.039 kb on - strand, within Pf6N2E2_112 at 158.053 kb on + strand, within Pf6N2E2_112 at 158.171 kb on + strand, within Pf6N2E2_112 at 158.212 kb on - strand, within Pf6N2E2_112 at 158.212 kb on - strand, within Pf6N2E2_112 at 158.236 kb on + strand, within Pf6N2E2_112 at 158.237 kb on - strand, within Pf6N2E2_112 at 158.461 kb on - strand at 158.576 kb on + strand, within Pf6N2E2_113 at 158.576 kb on + strand, within Pf6N2E2_113 at 158.577 kb on - strand, within Pf6N2E2_113 at 158.943 kb on + strand, within Pf6N2E2_113 at 159.166 kb on + strand, within Pf6N2E2_113 at 159.166 kb on + strand, within Pf6N2E2_113 at 159.167 kb on - strand, within Pf6N2E2_113 at 159.167 kb on - strand, within Pf6N2E2_113 at 159.201 kb on + strand, within Pf6N2E2_113 at 159.202 kb on - strand, within Pf6N2E2_113 at 159.235 kb on - strand, within Pf6N2E2_113 at 159.241 kb on + strand, within Pf6N2E2_113 at 159.441 kb on + strand, within Pf6N2E2_113 at 159.441 kb on + strand, within Pf6N2E2_113 at 159.441 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.444 kb on + strand, within Pf6N2E2_113 at 159.445 kb on - strand, within Pf6N2E2_113 at 159.445 kb on - strand, within Pf6N2E2_113 at 159.445 kb on - strand, within Pf6N2E2_113 at 159.507 kb on + strand, within Pf6N2E2_113 at 159.521 kb on - strand, within Pf6N2E2_113 at 159.735 kb on + strand, within Pf6N2E2_113 at 159.864 kb on + strand, within Pf6N2E2_113 at 159.864 kb on + strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.865 kb on - strand, within Pf6N2E2_113 at 159.955 kb on - strand, within Pf6N2E2_113 at 160.093 kb on - strand at 160.150 kb on - strand at 160.173 kb on + strand at 160.173 kb on + strand at 160.173 kb on + strand at 160.297 kb on + strand at 160.387 kb on - strand at 160.427 kb on + strand at 160.428 kb on - strand at 160.460 kb on + strand at 160.461 kb on - strand, within Pf6N2E2_114 at 160.461 kb on - strand, within Pf6N2E2_114
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1.5 mM remove 157,462 + Pf6N2E2_111 0.86 +1.2 157,462 + Pf6N2E2_111 0.86 -0.5 157,462 + Pf6N2E2_111 0.86 -0.8 157,462 + Pf6N2E2_111 0.86 +0.3 157,463 - Pf6N2E2_111 0.87 -0.2 157,559 + +0.6 157,664 + -1.0 157,665 - -0.4 157,694 - +1.1 157,736 - Pf6N2E2_112 0.16 +2.8 157,776 + Pf6N2E2_112 0.22 +0.4 157,776 + Pf6N2E2_112 0.22 +1.4 157,776 + Pf6N2E2_112 0.22 -0.7 157,776 + Pf6N2E2_112 0.22 -2.0 157,776 + Pf6N2E2_112 0.22 -1.1 157,776 + Pf6N2E2_112 0.22 -0.7 157,776 + Pf6N2E2_112 0.22 +0.5 157,776 + Pf6N2E2_112 0.22 +0.2 157,777 - Pf6N2E2_112 0.22 -0.5 157,777 - Pf6N2E2_112 0.22 +0.1 157,777 - Pf6N2E2_112 0.22 +0.2 157,777 - Pf6N2E2_112 0.22 +0.8 157,777 - Pf6N2E2_112 0.22 +0.0 157,777 - Pf6N2E2_112 0.22 -0.8 157,777 - Pf6N2E2_112 0.22 -0.0 157,777 - Pf6N2E2_112 0.22 -0.2 157,778 + Pf6N2E2_112 0.22 +0.3 157,778 + Pf6N2E2_112 0.22 -0.0 157,779 - Pf6N2E2_112 0.22 +0.0 157,806 + Pf6N2E2_112 0.26 -0.2 157,806 + Pf6N2E2_112 0.26 -1.2 157,806 + Pf6N2E2_112 0.26 +0.1 157,807 - Pf6N2E2_112 0.26 -0.3 157,934 - Pf6N2E2_112 0.45 +0.6 157,984 + Pf6N2E2_112 0.52 +0.8 158,038 + Pf6N2E2_112 0.60 +0.2 158,038 + Pf6N2E2_112 0.60 +0.2 158,039 - Pf6N2E2_112 0.60 -1.0 158,053 + Pf6N2E2_112 0.62 +1.3 158,171 + Pf6N2E2_112 0.79 +0.8 158,212 - Pf6N2E2_112 0.85 -0.3 158,212 - Pf6N2E2_112 0.85 +1.2 158,236 + Pf6N2E2_112 0.88 +0.4 158,237 - Pf6N2E2_112 0.89 -1.2 158,461 - -0.2 158,576 + Pf6N2E2_113 0.14 -0.8 158,576 + Pf6N2E2_113 0.14 +0.0 158,577 - Pf6N2E2_113 0.14 -0.0 158,943 + Pf6N2E2_113 0.33 +0.5 159,166 + Pf6N2E2_113 0.45 -0.0 159,166 + Pf6N2E2_113 0.45 +0.2 159,167 - Pf6N2E2_113 0.45 +0.2 159,167 - Pf6N2E2_113 0.45 +0.7 159,201 + Pf6N2E2_113 0.46 +0.8 159,202 - Pf6N2E2_113 0.46 -0.2 159,235 - Pf6N2E2_113 0.48 +0.4 159,241 + Pf6N2E2_113 0.49 +0.4 159,441 + Pf6N2E2_113 0.59 -0.4 159,441 + Pf6N2E2_113 0.59 -0.2 159,441 + Pf6N2E2_113 0.59 +1.8 159,444 + Pf6N2E2_113 0.59 +0.5 159,444 + Pf6N2E2_113 0.59 -0.6 159,444 + Pf6N2E2_113 0.59 -0.2 159,444 + Pf6N2E2_113 0.59 -0.2 159,445 - Pf6N2E2_113 0.59 +0.0 159,445 - Pf6N2E2_113 0.59 -0.4 159,445 - Pf6N2E2_113 0.59 +0.8 159,507 + Pf6N2E2_113 0.62 -0.3 159,521 - Pf6N2E2_113 0.63 -0.5 159,735 + Pf6N2E2_113 0.74 +1.3 159,864 + Pf6N2E2_113 0.81 -0.8 159,864 + Pf6N2E2_113 0.81 -0.2 159,865 - Pf6N2E2_113 0.81 -0.2 159,865 - Pf6N2E2_113 0.81 +0.8 159,865 - Pf6N2E2_113 0.81 -0.0 159,865 - Pf6N2E2_113 0.81 +1.6 159,955 - Pf6N2E2_113 0.86 -0.3 160,093 - +0.7 160,150 - +0.8 160,173 + +1.2 160,173 + -0.4 160,173 + +0.5 160,297 + +2.2 160,387 - +0.2 160,427 + +0.8 160,428 - +0.2 160,460 + -0.8 160,461 - Pf6N2E2_114 0.10 -0.2 160,461 - Pf6N2E2_114 0.10 -0.5
Or see this region's nucleotide sequence