Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS20715 and HSERO_RS20720 are separated by 4 nucleotides HSERO_RS20720 and HSERO_RS20725 overlap by 4 nucleotides HSERO_RS20725 and HSERO_RS20730 are separated by 21 nucleotides
HSERO_RS20715: HSERO_RS20715 - cell division protein FtsY, at 4,747,817 to 4,748,782
_RS20715
HSERO_RS20720: HSERO_RS20720 - GMP synthase, at 4,748,787 to 4,749,479
_RS20720
HSERO_RS20725: HSERO_RS20725 - permease, at 4,749,476 to 4,750,399
_RS20725
HSERO_RS20730: HSERO_RS20730 - hypothetical protein, at 4,750,421 to 4,751,362
_RS20730
Position (kb) 4749
4750
4751 Strain fitness (log2 ratio) -1
0
1
2 at 4748.853 kb on - strand at 4748.926 kb on - strand, within HSERO_RS20720 at 4748.997 kb on - strand, within HSERO_RS20720 at 4749.152 kb on + strand, within HSERO_RS20720 at 4749.152 kb on + strand, within HSERO_RS20720 at 4749.160 kb on - strand, within HSERO_RS20720 at 4749.165 kb on - strand, within HSERO_RS20720 at 4749.178 kb on - strand, within HSERO_RS20720 at 4749.242 kb on + strand, within HSERO_RS20720 at 4749.293 kb on - strand, within HSERO_RS20720 at 4749.340 kb on + strand, within HSERO_RS20720 at 4749.353 kb on - strand, within HSERO_RS20720 at 4749.592 kb on - strand, within HSERO_RS20725 at 4749.592 kb on - strand, within HSERO_RS20725 at 4749.616 kb on - strand, within HSERO_RS20725 at 4749.799 kb on + strand, within HSERO_RS20725 at 4749.858 kb on + strand, within HSERO_RS20725 at 4749.866 kb on - strand, within HSERO_RS20725 at 4749.906 kb on + strand, within HSERO_RS20725 at 4749.914 kb on - strand, within HSERO_RS20725 at 4750.040 kb on - strand, within HSERO_RS20725 at 4750.066 kb on + strand, within HSERO_RS20725 at 4750.074 kb on - strand, within HSERO_RS20725 at 4750.116 kb on - strand, within HSERO_RS20725 at 4750.232 kb on + strand, within HSERO_RS20725 at 4750.359 kb on - strand at 4750.477 kb on + strand at 4750.477 kb on + strand at 4750.637 kb on + strand, within HSERO_RS20730 at 4750.766 kb on - strand, within HSERO_RS20730 at 4750.817 kb on - strand, within HSERO_RS20730 at 4750.817 kb on - strand, within HSERO_RS20730 at 4750.850 kb on + strand, within HSERO_RS20730 at 4750.850 kb on + strand, within HSERO_RS20730 at 4750.891 kb on - strand, within HSERO_RS20730 at 4750.957 kb on - strand, within HSERO_RS20730 at 4750.973 kb on + strand, within HSERO_RS20730 at 4751.068 kb on + strand, within HSERO_RS20730 at 4751.229 kb on - strand, within HSERO_RS20730 at 4751.297 kb on + strand at 4751.388 kb on + strand at 4751.388 kb on + strand at 4751.388 kb on + strand at 4751.396 kb on - strand at 4751.396 kb on - strand at 4751.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,748,853 - +0.1 4,748,926 - HSERO_RS20720 0.20 +0.4 4,748,997 - HSERO_RS20720 0.30 -0.0 4,749,152 + HSERO_RS20720 0.53 -0.3 4,749,152 + HSERO_RS20720 0.53 -0.2 4,749,160 - HSERO_RS20720 0.54 -0.0 4,749,165 - HSERO_RS20720 0.55 +1.6 4,749,178 - HSERO_RS20720 0.56 +2.2 4,749,242 + HSERO_RS20720 0.66 -0.5 4,749,293 - HSERO_RS20720 0.73 -0.1 4,749,340 + HSERO_RS20720 0.80 +0.3 4,749,353 - HSERO_RS20720 0.82 -0.3 4,749,592 - HSERO_RS20725 0.13 -0.6 4,749,592 - HSERO_RS20725 0.13 -1.7 4,749,616 - HSERO_RS20725 0.15 -0.0 4,749,799 + HSERO_RS20725 0.35 -0.2 4,749,858 + HSERO_RS20725 0.41 -0.5 4,749,866 - HSERO_RS20725 0.42 -0.4 4,749,906 + HSERO_RS20725 0.47 +0.5 4,749,914 - HSERO_RS20725 0.47 -0.3 4,750,040 - HSERO_RS20725 0.61 -0.7 4,750,066 + HSERO_RS20725 0.64 -1.1 4,750,074 - HSERO_RS20725 0.65 -0.1 4,750,116 - HSERO_RS20725 0.69 -0.4 4,750,232 + HSERO_RS20725 0.82 +0.3 4,750,359 - +0.3 4,750,477 + +0.4 4,750,477 + +1.6 4,750,637 + HSERO_RS20730 0.23 -0.3 4,750,766 - HSERO_RS20730 0.37 -0.2 4,750,817 - HSERO_RS20730 0.42 -0.6 4,750,817 - HSERO_RS20730 0.42 -0.5 4,750,850 + HSERO_RS20730 0.46 +1.2 4,750,850 + HSERO_RS20730 0.46 -1.5 4,750,891 - HSERO_RS20730 0.50 +0.4 4,750,957 - HSERO_RS20730 0.57 +0.1 4,750,973 + HSERO_RS20730 0.59 +0.3 4,751,068 + HSERO_RS20730 0.69 -1.5 4,751,229 - HSERO_RS20730 0.86 -0.1 4,751,297 + +0.9 4,751,388 + -0.0 4,751,388 + -1.4 4,751,388 + -1.3 4,751,396 - +0.2 4,751,396 - -0.7 4,751,396 - +0.2
Or see this region's nucleotide sequence