Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22975 and BWI76_RS22980 are separated by 105 nucleotides BWI76_RS22980 and BWI76_RS22985 are separated by 211 nucleotides BWI76_RS22985 and BWI76_RS22990 are separated by 47 nucleotides BWI76_RS22990 and BWI76_RS22995 are separated by 44 nucleotides BWI76_RS22995 and BWI76_RS23000 are separated by 100 nucleotides
BWI76_RS22975: BWI76_RS22975 - tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA, at 4,796,200 to 4,797,009
_RS22975
BWI76_RS22980: BWI76_RS22980 - murein transglycosylase A, at 4,797,115 to 4,798,212
_RS22980
BWI76_RS22985: BWI76_RS22985 - tRNA-Met, at 4,798,424 to 4,798,500
_RS22985
BWI76_RS22990: BWI76_RS22990 - tRNA-Met, at 4,798,548 to 4,798,624
_RS22990
BWI76_RS22995: BWI76_RS22995 - tRNA-Met, at 4,798,669 to 4,798,745
_RS22995
BWI76_RS23000: BWI76_RS23000 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 4,798,846 to 4,799,901
_RS23000
Position (kb) 4797
4798
4799 Strain fitness (log2 ratio) -1
0
1
2 at 4796.151 kb on + strand at 4796.421 kb on - strand, within BWI76_RS22975 at 4796.438 kb on + strand, within BWI76_RS22975 at 4796.649 kb on - strand, within BWI76_RS22975 at 4796.985 kb on + strand at 4797.213 kb on - strand at 4797.261 kb on + strand, within BWI76_RS22980 at 4797.261 kb on + strand, within BWI76_RS22980 at 4797.269 kb on - strand, within BWI76_RS22980 at 4797.285 kb on + strand, within BWI76_RS22980 at 4797.370 kb on + strand, within BWI76_RS22980 at 4797.424 kb on - strand, within BWI76_RS22980 at 4797.987 kb on - strand, within BWI76_RS22980 at 4797.989 kb on + strand, within BWI76_RS22980 at 4798.027 kb on - strand, within BWI76_RS22980 at 4798.112 kb on + strand at 4798.245 kb on - strand at 4799.001 kb on + strand, within BWI76_RS23000 at 4799.051 kb on - strand, within BWI76_RS23000
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 4,796,151 + -0.8 4,796,421 - BWI76_RS22975 0.27 -0.9 4,796,438 + BWI76_RS22975 0.29 +0.8 4,796,649 - BWI76_RS22975 0.55 -0.9 4,796,985 + -0.3 4,797,213 - +0.6 4,797,261 + BWI76_RS22980 0.13 +1.4 4,797,261 + BWI76_RS22980 0.13 +0.3 4,797,269 - BWI76_RS22980 0.14 +0.2 4,797,285 + BWI76_RS22980 0.15 +0.6 4,797,370 + BWI76_RS22980 0.23 +0.9 4,797,424 - BWI76_RS22980 0.28 +1.5 4,797,987 - BWI76_RS22980 0.79 -0.9 4,797,989 + BWI76_RS22980 0.80 +2.2 4,798,027 - BWI76_RS22980 0.83 +1.4 4,798,112 + +0.7 4,798,245 - -0.1 4,799,001 + BWI76_RS23000 0.15 +0.9 4,799,051 - BWI76_RS23000 0.19 +0.4
Or see this region's nucleotide sequence