Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17055 and BWI76_RS17065 are separated by 400 nucleotides BWI76_RS17065 and BWI76_RS17070 are separated by 342 nucleotides
BWI76_RS17055: BWI76_RS17055 - hypothetical protein, at 3,538,538 to 3,539,659
_RS17055
BWI76_RS17065: BWI76_RS17065 - phosphoenolpyruvate synthase, at 3,540,060 to 3,542,438
_RS17065
BWI76_RS17070: BWI76_RS17070 - phosphoenolpyruvate synthase regulatory protein, at 3,542,781 to 3,543,614
_RS17070
Position (kb) 3540
3541
3542
3543 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3539.214 kb on - strand, within BWI76_RS17055 at 3539.214 kb on - strand, within BWI76_RS17055 at 3539.232 kb on + strand, within BWI76_RS17055 at 3539.240 kb on - strand, within BWI76_RS17055 at 3539.325 kb on - strand, within BWI76_RS17055 at 3539.439 kb on - strand, within BWI76_RS17055 at 3539.555 kb on - strand at 3539.569 kb on + strand at 3539.706 kb on - strand at 3539.940 kb on + strand at 3539.940 kb on + strand at 3540.597 kb on - strand, within BWI76_RS17065 at 3540.799 kb on + strand, within BWI76_RS17065 at 3540.800 kb on - strand, within BWI76_RS17065 at 3540.880 kb on + strand, within BWI76_RS17065 at 3541.317 kb on + strand, within BWI76_RS17065 at 3541.393 kb on - strand, within BWI76_RS17065 at 3541.393 kb on - strand, within BWI76_RS17065 at 3541.393 kb on - strand, within BWI76_RS17065 at 3541.393 kb on - strand, within BWI76_RS17065 at 3541.444 kb on + strand, within BWI76_RS17065 at 3541.452 kb on - strand, within BWI76_RS17065 at 3541.617 kb on + strand, within BWI76_RS17065 at 3541.630 kb on - strand, within BWI76_RS17065 at 3542.225 kb on + strand at 3542.322 kb on + strand at 3542.363 kb on + strand at 3542.368 kb on + strand at 3542.381 kb on - strand at 3542.381 kb on - strand at 3542.381 kb on - strand at 3542.381 kb on - strand at 3542.391 kb on + strand at 3542.396 kb on + strand at 3542.396 kb on + strand at 3542.404 kb on - strand at 3542.404 kb on - strand at 3542.411 kb on - strand at 3542.428 kb on + strand at 3542.430 kb on + strand at 3542.430 kb on + strand at 3542.444 kb on - strand at 3542.496 kb on + strand at 3542.510 kb on + strand at 3542.581 kb on + strand at 3542.600 kb on - strand at 3542.761 kb on + strand at 3542.788 kb on + strand at 3542.788 kb on + strand at 3542.788 kb on + strand at 3542.788 kb on + strand at 3542.796 kb on - strand at 3542.796 kb on - strand at 3542.844 kb on + strand at 3542.844 kb on + strand at 3542.844 kb on + strand at 3542.877 kb on + strand, within BWI76_RS17070 at 3542.885 kb on + strand, within BWI76_RS17070 at 3542.890 kb on + strand, within BWI76_RS17070 at 3542.940 kb on + strand, within BWI76_RS17070 at 3543.189 kb on - strand, within BWI76_RS17070 at 3543.220 kb on + strand, within BWI76_RS17070
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 3,539,214 - BWI76_RS17055 0.60 +0.0 3,539,214 - BWI76_RS17055 0.60 -0.3 3,539,232 + BWI76_RS17055 0.62 +0.3 3,539,240 - BWI76_RS17055 0.63 +0.8 3,539,325 - BWI76_RS17055 0.70 +0.7 3,539,439 - BWI76_RS17055 0.80 +0.9 3,539,555 - +0.4 3,539,569 + -0.4 3,539,706 - +0.2 3,539,940 + -0.2 3,539,940 + +0.5 3,540,597 - BWI76_RS17065 0.23 +0.6 3,540,799 + BWI76_RS17065 0.31 +0.7 3,540,800 - BWI76_RS17065 0.31 +1.0 3,540,880 + BWI76_RS17065 0.34 +0.8 3,541,317 + BWI76_RS17065 0.53 +1.8 3,541,393 - BWI76_RS17065 0.56 +0.3 3,541,393 - BWI76_RS17065 0.56 +0.8 3,541,393 - BWI76_RS17065 0.56 +1.0 3,541,393 - BWI76_RS17065 0.56 +0.7 3,541,444 + BWI76_RS17065 0.58 +0.5 3,541,452 - BWI76_RS17065 0.59 +0.7 3,541,617 + BWI76_RS17065 0.65 +0.9 3,541,630 - BWI76_RS17065 0.66 +0.6 3,542,225 + +0.8 3,542,322 + +1.3 3,542,363 + -1.0 3,542,368 + +0.4 3,542,381 - +0.5 3,542,381 - +0.8 3,542,381 - +0.5 3,542,381 - -0.6 3,542,391 + -2.9 3,542,396 + -1.8 3,542,396 + +1.1 3,542,404 - +0.8 3,542,404 - +1.1 3,542,411 - +0.7 3,542,428 + +0.2 3,542,430 + -2.9 3,542,430 + -3.0 3,542,444 - +0.9 3,542,496 + +0.6 3,542,510 + -0.6 3,542,581 + -0.2 3,542,600 - +0.0 3,542,761 + -1.2 3,542,788 + -0.4 3,542,788 + +2.8 3,542,788 + +0.3 3,542,788 + -0.0 3,542,796 - -1.2 3,542,796 - -1.2 3,542,844 + -0.4 3,542,844 + -0.2 3,542,844 + -0.0 3,542,877 + BWI76_RS17070 0.12 -0.2 3,542,885 + BWI76_RS17070 0.12 +0.8 3,542,890 + BWI76_RS17070 0.13 +1.0 3,542,940 + BWI76_RS17070 0.19 +0.5 3,543,189 - BWI76_RS17070 0.49 +0.6 3,543,220 + BWI76_RS17070 0.53 +1.2
Or see this region's nucleotide sequence