Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03865 and BWI76_RS03870 overlap by 4 nucleotides BWI76_RS03870 and BWI76_RS03875 overlap by 4 nucleotides BWI76_RS03875 and BWI76_RS03880 are separated by 284 nucleotides
BWI76_RS03865: BWI76_RS03865 - 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase, at 799,174 to 800,640
_RS03865
BWI76_RS03870: BWI76_RS03870 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 800,637 to 801,401
_RS03870
BWI76_RS03875: BWI76_RS03875 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase, at 801,398 to 802,030
_RS03875
BWI76_RS03880: BWI76_RS03880 - homoprotocatechuate degradation operon regulator, HpaR, at 802,315 to 802,752
_RS03880
Position (kb) 800
801
802 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 799.832 kb on + strand, within BWI76_RS03865 at 799.840 kb on - strand, within BWI76_RS03865 at 799.850 kb on - strand, within BWI76_RS03865 at 800.010 kb on + strand, within BWI76_RS03865 at 800.136 kb on - strand, within BWI76_RS03865 at 800.392 kb on - strand, within BWI76_RS03865 at 800.579 kb on - strand at 800.888 kb on - strand, within BWI76_RS03870 at 801.013 kb on + strand, within BWI76_RS03870 at 801.133 kb on - strand, within BWI76_RS03870 at 801.172 kb on - strand, within BWI76_RS03870 at 801.244 kb on - strand, within BWI76_RS03870 at 801.246 kb on + strand, within BWI76_RS03870 at 801.249 kb on - strand, within BWI76_RS03870 at 801.255 kb on - strand, within BWI76_RS03870 at 801.351 kb on + strand at 801.494 kb on + strand, within BWI76_RS03875 at 801.651 kb on + strand, within BWI76_RS03875 at 801.950 kb on - strand, within BWI76_RS03875 at 801.957 kb on + strand, within BWI76_RS03875 at 802.313 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 799,832 + BWI76_RS03865 0.45 -0.1 799,840 - BWI76_RS03865 0.45 +0.4 799,850 - BWI76_RS03865 0.46 -0.4 800,010 + BWI76_RS03865 0.57 +0.5 800,136 - BWI76_RS03865 0.66 +0.7 800,392 - BWI76_RS03865 0.83 +0.4 800,579 - +0.4 800,888 - BWI76_RS03870 0.33 +0.7 801,013 + BWI76_RS03870 0.49 -0.3 801,133 - BWI76_RS03870 0.65 +0.6 801,172 - BWI76_RS03870 0.70 -1.0 801,244 - BWI76_RS03870 0.79 +1.1 801,246 + BWI76_RS03870 0.80 +0.6 801,249 - BWI76_RS03870 0.80 +0.6 801,255 - BWI76_RS03870 0.81 +0.4 801,351 + -0.2 801,494 + BWI76_RS03875 0.15 +0.4 801,651 + BWI76_RS03875 0.40 +0.5 801,950 - BWI76_RS03875 0.87 -0.6 801,957 + BWI76_RS03875 0.88 +0.5 802,313 - +4.1
Or see this region's nucleotide sequence