Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03020 and BWI76_RS03025 are separated by 99 nucleotides BWI76_RS03025 and BWI76_RS03030 are separated by 123 nucleotides BWI76_RS03030 and BWI76_RS03035 overlap by 1 nucleotides BWI76_RS03035 and BWI76_RS03040 are separated by 98 nucleotides
BWI76_RS03020: BWI76_RS03020 - tRNA hydroxylase, at 617,545 to 618,303
_RS03020
BWI76_RS03025: BWI76_RS03025 - N-acetyltransferase, at 618,403 to 618,906
_RS03025
BWI76_RS03030: BWI76_RS03030 - valine--tRNA ligase, at 619,030 to 621,885
_RS03030
BWI76_RS03035: BWI76_RS03035 - DNA polymerase III subunit chi, at 621,885 to 622,328
_RS03035
BWI76_RS03040: BWI76_RS03040 - leucyl aminopeptidase, at 622,427 to 623,938
_RS03040
Position (kb) 619
620
621
622 Strain fitness (log2 ratio) -1
0
1
2 at 618.331 kb on - strand at 618.410 kb on + strand at 618.565 kb on + strand, within BWI76_RS03025 at 618.573 kb on - strand, within BWI76_RS03025 at 618.605 kb on - strand, within BWI76_RS03025 at 618.884 kb on - strand at 618.885 kb on - strand at 618.966 kb on + strand at 622.466 kb on - strand at 622.850 kb on - strand, within BWI76_RS03040
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL remove 618,331 - +0.1 618,410 + -1.1 618,565 + BWI76_RS03025 0.32 +0.2 618,573 - BWI76_RS03025 0.34 +1.3 618,605 - BWI76_RS03025 0.40 -0.4 618,884 - +1.3 618,885 - +0.6 618,966 + +1.1 622,466 - +2.1 622,850 - BWI76_RS03040 0.28 +0.4
Or see this region's nucleotide sequence