Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27900 and BWI76_RS27905 are separated by 138 nucleotides BWI76_RS27905 and BWI76_RS27910 are separated by 118 nucleotides BWI76_RS27910 and BWI76_RS27915 are separated by 367 nucleotides BWI76_RS27915 and BWI76_RS27920 are separated by 1 nucleotides
BWI76_RS27900: BWI76_RS27900 - DNA damage-inducible protein D, at 5,787,280 to 5,788,106
_RS27900
BWI76_RS27905: BWI76_RS27905 - heat-shock protein IbpB, at 5,788,245 to 5,788,673
_RS27905
BWI76_RS27910: BWI76_RS27910 - heat-shock protein IbpA, at 5,788,792 to 5,789,205
_RS27910
BWI76_RS27915: BWI76_RS27915 - YceK/YidQ family lipoprotein, at 5,789,573 to 5,789,905
_RS27915
BWI76_RS27920: BWI76_RS27920 - hypothetical protein, at 5,789,907 to 5,791,133
_RS27920
Position (kb) 5788
5789
5790 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5787.845 kb on - strand, within BWI76_RS27900 at 5787.854 kb on + strand, within BWI76_RS27900 at 5787.871 kb on - strand, within BWI76_RS27900 at 5787.918 kb on - strand, within BWI76_RS27900 at 5787.924 kb on + strand, within BWI76_RS27900 at 5787.950 kb on + strand, within BWI76_RS27900 at 5788.015 kb on + strand, within BWI76_RS27900 at 5788.042 kb on + strand at 5788.050 kb on - strand at 5788.180 kb on + strand at 5788.399 kb on - strand, within BWI76_RS27905 at 5788.421 kb on - strand, within BWI76_RS27905 at 5788.531 kb on - strand, within BWI76_RS27905 at 5788.531 kb on - strand, within BWI76_RS27905 at 5788.566 kb on + strand, within BWI76_RS27905 at 5788.666 kb on - strand at 5788.675 kb on + strand at 5788.714 kb on - strand at 5788.846 kb on + strand, within BWI76_RS27910 at 5788.850 kb on + strand, within BWI76_RS27910 at 5788.850 kb on + strand, within BWI76_RS27910 at 5788.850 kb on + strand, within BWI76_RS27910 at 5788.850 kb on + strand, within BWI76_RS27910 at 5788.858 kb on - strand, within BWI76_RS27910 at 5788.858 kb on - strand, within BWI76_RS27910 at 5788.858 kb on - strand, within BWI76_RS27910 at 5788.858 kb on - strand, within BWI76_RS27910 at 5788.858 kb on - strand, within BWI76_RS27910 at 5788.858 kb on - strand, within BWI76_RS27910 at 5788.858 kb on - strand, within BWI76_RS27910 at 5788.864 kb on + strand, within BWI76_RS27910 at 5788.940 kb on + strand, within BWI76_RS27910 at 5788.947 kb on - strand, within BWI76_RS27910 at 5788.950 kb on + strand, within BWI76_RS27910 at 5788.951 kb on - strand, within BWI76_RS27910 at 5788.962 kb on + strand, within BWI76_RS27910 at 5788.985 kb on + strand, within BWI76_RS27910 at 5789.000 kb on + strand, within BWI76_RS27910 at 5789.008 kb on - strand, within BWI76_RS27910 at 5789.008 kb on - strand, within BWI76_RS27910 at 5789.063 kb on + strand, within BWI76_RS27910 at 5789.071 kb on - strand, within BWI76_RS27910 at 5789.095 kb on + strand, within BWI76_RS27910 at 5789.095 kb on + strand, within BWI76_RS27910 at 5789.095 kb on + strand, within BWI76_RS27910 at 5789.095 kb on + strand, within BWI76_RS27910 at 5789.111 kb on + strand, within BWI76_RS27910 at 5789.119 kb on - strand, within BWI76_RS27910 at 5789.119 kb on - strand, within BWI76_RS27910 at 5789.169 kb on - strand at 5789.207 kb on + strand at 5789.225 kb on - strand at 5789.238 kb on - strand at 5789.258 kb on + strand at 5789.258 kb on + strand at 5789.258 kb on + strand at 5789.323 kb on + strand at 5789.325 kb on - strand at 5789.331 kb on - strand at 5789.332 kb on - strand at 5789.332 kb on - strand at 5789.384 kb on + strand at 5789.387 kb on - strand at 5789.596 kb on - strand at 5789.600 kb on + strand at 5789.600 kb on + strand at 5789.747 kb on + strand, within BWI76_RS27915 at 5789.774 kb on - strand, within BWI76_RS27915 at 5789.819 kb on - strand at 5789.826 kb on + strand, within BWI76_RS27915 at 5789.915 kb on + strand at 5789.962 kb on + strand at 5790.021 kb on - strand at 5790.147 kb on + strand, within BWI76_RS27920 at 5790.175 kb on - strand, within BWI76_RS27920 at 5790.175 kb on - strand, within BWI76_RS27920 at 5790.177 kb on - strand, within BWI76_RS27920
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 5,787,845 - BWI76_RS27900 0.68 -1.3 5,787,854 + BWI76_RS27900 0.69 -0.5 5,787,871 - BWI76_RS27900 0.71 -0.1 5,787,918 - BWI76_RS27900 0.77 +1.7 5,787,924 + BWI76_RS27900 0.78 -0.4 5,787,950 + BWI76_RS27900 0.81 +1.0 5,788,015 + BWI76_RS27900 0.89 -1.0 5,788,042 + +1.3 5,788,050 - +0.6 5,788,180 + -0.2 5,788,399 - BWI76_RS27905 0.36 -0.7 5,788,421 - BWI76_RS27905 0.41 -0.1 5,788,531 - BWI76_RS27905 0.67 +0.5 5,788,531 - BWI76_RS27905 0.67 -0.2 5,788,566 + BWI76_RS27905 0.75 -0.4 5,788,666 - +1.0 5,788,675 + -0.2 5,788,714 - +0.2 5,788,846 + BWI76_RS27910 0.13 +0.5 5,788,850 + BWI76_RS27910 0.14 -1.3 5,788,850 + BWI76_RS27910 0.14 +0.1 5,788,850 + BWI76_RS27910 0.14 +0.5 5,788,850 + BWI76_RS27910 0.14 +1.1 5,788,858 - BWI76_RS27910 0.16 +0.7 5,788,858 - BWI76_RS27910 0.16 +0.1 5,788,858 - BWI76_RS27910 0.16 +0.4 5,788,858 - BWI76_RS27910 0.16 +0.1 5,788,858 - BWI76_RS27910 0.16 -0.0 5,788,858 - BWI76_RS27910 0.16 +0.2 5,788,858 - BWI76_RS27910 0.16 -0.2 5,788,864 + BWI76_RS27910 0.17 -1.3 5,788,940 + BWI76_RS27910 0.36 +1.2 5,788,947 - BWI76_RS27910 0.37 +1.0 5,788,950 + BWI76_RS27910 0.38 +1.3 5,788,951 - BWI76_RS27910 0.38 +2.2 5,788,962 + BWI76_RS27910 0.41 -1.0 5,788,985 + BWI76_RS27910 0.47 +0.4 5,789,000 + BWI76_RS27910 0.50 +1.7 5,789,008 - BWI76_RS27910 0.52 +1.4 5,789,008 - BWI76_RS27910 0.52 -1.3 5,789,063 + BWI76_RS27910 0.65 +1.0 5,789,071 - BWI76_RS27910 0.67 +0.5 5,789,095 + BWI76_RS27910 0.73 +0.5 5,789,095 + BWI76_RS27910 0.73 +0.2 5,789,095 + BWI76_RS27910 0.73 +1.5 5,789,095 + BWI76_RS27910 0.73 -1.3 5,789,111 + BWI76_RS27910 0.77 +0.3 5,789,119 - BWI76_RS27910 0.79 -3.3 5,789,119 - BWI76_RS27910 0.79 -0.2 5,789,169 - +0.2 5,789,207 + -0.5 5,789,225 - +0.1 5,789,238 - -0.1 5,789,258 + +0.9 5,789,258 + +0.3 5,789,258 + +0.4 5,789,323 + +0.5 5,789,325 - -0.1 5,789,331 - +0.3 5,789,332 - +1.0 5,789,332 - -0.4 5,789,384 + +0.8 5,789,387 - +0.4 5,789,596 - -0.8 5,789,600 + -0.1 5,789,600 + +0.7 5,789,747 + BWI76_RS27915 0.52 -0.4 5,789,774 - BWI76_RS27915 0.60 -0.6 5,789,819 - +1.9 5,789,826 + BWI76_RS27915 0.76 +1.1 5,789,915 + -1.3 5,789,962 + -0.2 5,790,021 - +0.2 5,790,147 + BWI76_RS27920 0.20 +0.7 5,790,175 - BWI76_RS27920 0.22 -0.5 5,790,175 - BWI76_RS27920 0.22 +0.2 5,790,177 - BWI76_RS27920 0.22 +0.1
Or see this region's nucleotide sequence