Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25145 and BWI76_RS25150 overlap by 11 nucleotides BWI76_RS25150 and BWI76_RS25155 overlap by 48 nucleotides BWI76_RS25155 and BWI76_RS25160 are separated by 152 nucleotides BWI76_RS25160 and BWI76_RS25165 are separated by 233 nucleotides
BWI76_RS25145: BWI76_RS25145 - 23S rRNA (uridine(2552)-2'-O)-methyltransferase, at 5,234,420 to 5,235,049
_RS25145
BWI76_RS25150: BWI76_RS25150 - hypothetical protein, at 5,235,039 to 5,235,221
_RS25150
BWI76_RS25155: BWI76_RS25155 - RNA-binding protein, at 5,235,174 to 5,235,467
_RS25155
BWI76_RS25160: BWI76_RS25160 - transcription elongation factor GreA, at 5,235,620 to 5,236,096
_RS25160
BWI76_RS25165: BWI76_RS25165 - serine-type D-Ala-D-Ala carboxypeptidase, at 5,236,330 to 5,237,763
_RS25165
Position (kb) 5235
5236
5237 Strain fitness (log2 ratio) -1
0
1 at 5235.956 kb on - strand, within BWI76_RS25160 at 5236.079 kb on + strand at 5236.129 kb on + strand at 5236.208 kb on - strand at 5236.375 kb on - strand at 5236.414 kb on - strand at 5236.548 kb on + strand, within BWI76_RS25165 at 5236.617 kb on + strand, within BWI76_RS25165 at 5236.727 kb on + strand, within BWI76_RS25165 at 5236.752 kb on - strand, within BWI76_RS25165 at 5236.868 kb on - strand, within BWI76_RS25165
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 5,235,956 - BWI76_RS25160 0.70 +1.6 5,236,079 + -0.4 5,236,129 + +0.4 5,236,208 - -0.5 5,236,375 - +0.7 5,236,414 - +0.8 5,236,548 + BWI76_RS25165 0.15 -0.7 5,236,617 + BWI76_RS25165 0.20 +0.1 5,236,727 + BWI76_RS25165 0.28 +0.1 5,236,752 - BWI76_RS25165 0.29 +0.2 5,236,868 - BWI76_RS25165 0.38 +0.6
Or see this region's nucleotide sequence