Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24235 and BWI76_RS24240 are separated by 117 nucleotides BWI76_RS24240 and BWI76_RS24245 overlap by 59 nucleotides BWI76_RS24245 and BWI76_RS24250 are separated by 204 nucleotides BWI76_RS24250 and BWI76_RS24255 are separated by 60 nucleotides
BWI76_RS24235: BWI76_RS24235 - Branched-chain-amino-acid transaminase (EC 2.6.1.42) <small>(from data)</small>, at 5,050,849 to 5,052,030
_RS24235
BWI76_RS24240: BWI76_RS24240 - hypothetical protein, at 5,052,148 to 5,052,354
_RS24240
BWI76_RS24245: BWI76_RS24245 - bifunctional glutathionylspermidine amidase/glutathionylspermidine synthase, at 5,052,296 to 5,054,167
_RS24245
BWI76_RS24250: BWI76_RS24250 - hypothetical protein, at 5,054,372 to 5,054,932
_RS24250
BWI76_RS24255: BWI76_RS24255 - thiol:disulfide oxidoreductase, at 5,054,993 to 5,055,859
_RS24255
Position (kb) 5052
5053
5054
5055 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5051.542 kb on - strand, within BWI76_RS24235 at 5051.806 kb on - strand, within BWI76_RS24235 at 5051.860 kb on + strand, within BWI76_RS24235 at 5051.869 kb on + strand, within BWI76_RS24235 at 5051.869 kb on - strand, within BWI76_RS24235 at 5051.892 kb on - strand, within BWI76_RS24235 at 5051.947 kb on + strand at 5051.959 kb on - strand at 5051.970 kb on - strand at 5051.987 kb on + strand at 5052.194 kb on + strand, within BWI76_RS24240 at 5052.414 kb on + strand at 5052.453 kb on + strand at 5052.818 kb on - strand, within BWI76_RS24245 at 5052.977 kb on - strand, within BWI76_RS24245 at 5053.002 kb on + strand, within BWI76_RS24245 at 5053.021 kb on + strand, within BWI76_RS24245 at 5053.088 kb on + strand, within BWI76_RS24245 at 5053.497 kb on + strand, within BWI76_RS24245 at 5053.673 kb on - strand, within BWI76_RS24245 at 5053.819 kb on - strand, within BWI76_RS24245 at 5053.824 kb on - strand, within BWI76_RS24245 at 5054.009 kb on + strand at 5054.117 kb on + strand at 5054.117 kb on + strand at 5054.125 kb on - strand at 5054.125 kb on - strand at 5054.274 kb on - strand at 5054.407 kb on + strand at 5054.415 kb on - strand at 5054.438 kb on - strand, within BWI76_RS24250 at 5054.452 kb on + strand, within BWI76_RS24250 at 5054.485 kb on + strand, within BWI76_RS24250 at 5054.636 kb on - strand, within BWI76_RS24250 at 5054.676 kb on + strand, within BWI76_RS24250 at 5055.025 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 5,051,542 - BWI76_RS24235 0.59 -3.1 5,051,806 - BWI76_RS24235 0.81 +0.1 5,051,860 + BWI76_RS24235 0.86 +0.0 5,051,869 + BWI76_RS24235 0.86 -1.6 5,051,869 - BWI76_RS24235 0.86 -2.2 5,051,892 - BWI76_RS24235 0.88 -0.0 5,051,947 + -0.0 5,051,959 - +0.2 5,051,970 - +0.0 5,051,987 + +0.3 5,052,194 + BWI76_RS24240 0.22 +0.5 5,052,414 + -1.2 5,052,453 + +0.7 5,052,818 - BWI76_RS24245 0.28 +0.7 5,052,977 - BWI76_RS24245 0.36 +0.5 5,053,002 + BWI76_RS24245 0.38 -0.5 5,053,021 + BWI76_RS24245 0.39 +0.9 5,053,088 + BWI76_RS24245 0.42 +0.4 5,053,497 + BWI76_RS24245 0.64 -0.2 5,053,673 - BWI76_RS24245 0.74 -0.3 5,053,819 - BWI76_RS24245 0.81 -0.2 5,053,824 - BWI76_RS24245 0.82 -1.1 5,054,009 + +2.4 5,054,117 + +0.9 5,054,117 + -0.1 5,054,125 - +0.6 5,054,125 - +0.3 5,054,274 - +0.7 5,054,407 + -1.6 5,054,415 - +1.3 5,054,438 - BWI76_RS24250 0.12 +0.6 5,054,452 + BWI76_RS24250 0.14 -0.1 5,054,485 + BWI76_RS24250 0.20 +0.5 5,054,636 - BWI76_RS24250 0.47 -0.6 5,054,676 + BWI76_RS24250 0.54 +0.1 5,055,025 - -0.3
Or see this region's nucleotide sequence