Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15535 and BWI76_RS15540 overlap by 1 nucleotides BWI76_RS15540 and BWI76_RS15545 are separated by 117 nucleotides BWI76_RS15545 and BWI76_RS15550 are separated by 40 nucleotides BWI76_RS15550 and BWI76_RS15555 are separated by 91 nucleotides BWI76_RS15555 and BWI76_RS15560 overlap by 4 nucleotides BWI76_RS15560 and BWI76_RS15565 are separated by 74 nucleotides
BWI76_RS15535: BWI76_RS15535 - hypothetical protein, at 3,255,560 to 3,256,030
_RS15535
BWI76_RS15540: BWI76_RS15540 - hypothetical protein, at 3,256,030 to 3,256,506
_RS15540
BWI76_RS15545: BWI76_RS15545 - hypothetical protein, at 3,256,624 to 3,256,911
_RS15545
BWI76_RS15550: BWI76_RS15550 - hypothetical protein, at 3,256,952 to 3,259,612
_RS15550
BWI76_RS15555: BWI76_RS15555 - hypothetical protein, at 3,259,704 to 3,260,174
_RS15555
BWI76_RS15560: BWI76_RS15560 - hypothetical protein, at 3,260,171 to 3,260,503
_RS15560
BWI76_RS15565: BWI76_RS15565 - hypothetical protein, at 3,260,578 to 3,260,790
_RS15565
Position (kb)
3256
3257
3258
3259
3260 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3255.979 kb on + strand, within BWI76_RS15535 at 3255.987 kb on - strand at 3256.015 kb on + strand at 3256.131 kb on + strand, within BWI76_RS15540 at 3256.328 kb on + strand, within BWI76_RS15540 at 3256.375 kb on - strand, within BWI76_RS15540 at 3256.428 kb on + strand, within BWI76_RS15540 at 3256.433 kb on + strand, within BWI76_RS15540 at 3256.433 kb on + strand, within BWI76_RS15540 at 3256.441 kb on - strand, within BWI76_RS15540 at 3256.450 kb on + strand, within BWI76_RS15540 at 3256.623 kb on - strand at 3256.664 kb on + strand, within BWI76_RS15545 at 3256.693 kb on + strand, within BWI76_RS15545 at 3256.708 kb on - strand, within BWI76_RS15545 at 3256.796 kb on + strand, within BWI76_RS15545 at 3256.836 kb on + strand, within BWI76_RS15545 at 3256.944 kb on + strand at 3257.091 kb on - strand at 3257.216 kb on - strand at 3257.334 kb on + strand, within BWI76_RS15550 at 3257.404 kb on - strand, within BWI76_RS15550 at 3257.587 kb on + strand, within BWI76_RS15550 at 3257.632 kb on - strand, within BWI76_RS15550 at 3257.632 kb on - strand, within BWI76_RS15550 at 3257.697 kb on - strand, within BWI76_RS15550 at 3258.102 kb on - strand, within BWI76_RS15550 at 3258.148 kb on + strand, within BWI76_RS15550 at 3258.510 kb on + strand, within BWI76_RS15550 at 3258.621 kb on - strand, within BWI76_RS15550 at 3258.627 kb on + strand at 3258.663 kb on - strand, within BWI76_RS15550 at 3258.663 kb on - strand, within BWI76_RS15550 at 3258.729 kb on + strand, within BWI76_RS15550 at 3258.729 kb on + strand, within BWI76_RS15550 at 3258.860 kb on + strand, within BWI76_RS15550 at 3258.873 kb on + strand, within BWI76_RS15550 at 3259.002 kb on + strand, within BWI76_RS15550 at 3259.088 kb on + strand, within BWI76_RS15550 at 3259.106 kb on - strand, within BWI76_RS15550 at 3259.151 kb on - strand, within BWI76_RS15550 at 3259.174 kb on - strand, within BWI76_RS15550 at 3259.255 kb on + strand, within BWI76_RS15550 at 3259.333 kb on + strand, within BWI76_RS15550 at 3259.362 kb on + strand at 3259.362 kb on + strand at 3259.370 kb on - strand at 3259.370 kb on - strand at 3259.370 kb on - strand at 3259.390 kb on + strand at 3259.399 kb on - strand at 3259.414 kb on - strand at 3259.418 kb on + strand at 3259.425 kb on + strand at 3259.426 kb on - strand at 3259.449 kb on + strand at 3259.449 kb on + strand at 3259.457 kb on - strand at 3259.457 kb on - strand at 3259.457 kb on - strand at 3259.480 kb on + strand at 3259.535 kb on + strand at 3259.535 kb on + strand at 3259.535 kb on + strand at 3259.543 kb on - strand at 3259.606 kb on + strand at 3259.607 kb on + strand at 3259.608 kb on + strand at 3259.608 kb on + strand at 3259.689 kb on + strand at 3259.708 kb on - strand at 3259.749 kb on + strand at 3259.757 kb on - strand, within BWI76_RS15555 at 3259.757 kb on - strand, within BWI76_RS15555 at 3259.757 kb on - strand, within BWI76_RS15555 at 3259.757 kb on - strand, within BWI76_RS15555 at 3259.757 kb on - strand, within BWI76_RS15555 at 3259.765 kb on - strand, within BWI76_RS15555 at 3259.776 kb on - strand, within BWI76_RS15555 at 3259.776 kb on - strand, within BWI76_RS15555 at 3259.790 kb on - strand, within BWI76_RS15555 at 3259.848 kb on - strand, within BWI76_RS15555 at 3259.889 kb on - strand, within BWI76_RS15555 at 3259.891 kb on + strand, within BWI76_RS15555 at 3259.901 kb on - strand, within BWI76_RS15555 at 3259.935 kb on - strand, within BWI76_RS15555 at 3259.985 kb on - strand, within BWI76_RS15555 at 3260.007 kb on - strand, within BWI76_RS15555 at 3260.007 kb on - strand, within BWI76_RS15555 at 3260.024 kb on - strand, within BWI76_RS15555 at 3260.024 kb on - strand, within BWI76_RS15555 at 3260.043 kb on - strand, within BWI76_RS15555 at 3260.069 kb on + strand, within BWI76_RS15555 at 3260.077 kb on - strand, within BWI76_RS15555 at 3260.077 kb on - strand, within BWI76_RS15555 at 3260.184 kb on - strand at 3260.184 kb on - strand at 3260.241 kb on - strand, within BWI76_RS15560 at 3260.248 kb on + strand, within BWI76_RS15560 at 3260.256 kb on - strand, within BWI76_RS15560 at 3260.256 kb on - strand, within BWI76_RS15560 at 3260.267 kb on - strand, within BWI76_RS15560 at 3260.269 kb on + strand, within BWI76_RS15560 at 3260.311 kb on - strand, within BWI76_RS15560 at 3260.339 kb on + strand, within BWI76_RS15560 at 3260.339 kb on + strand, within BWI76_RS15560 at 3260.339 kb on + strand, within BWI76_RS15560 at 3260.339 kb on + strand, within BWI76_RS15560 at 3260.339 kb on + strand, within BWI76_RS15560 at 3260.339 kb on + strand, within BWI76_RS15560 at 3260.347 kb on - strand, within BWI76_RS15560 at 3260.347 kb on - strand, within BWI76_RS15560 at 3260.347 kb on - strand, within BWI76_RS15560 at 3260.347 kb on - strand, within BWI76_RS15560 at 3260.347 kb on - strand, within BWI76_RS15560 at 3260.347 kb on - strand, within BWI76_RS15560 at 3260.348 kb on - strand, within BWI76_RS15560 at 3260.496 kb on - strand at 3260.552 kb on - strand at 3260.583 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 3,255,979 + BWI76_RS15535 0.89 -1.0 3,255,987 - -0.9 3,256,015 + -1.7 3,256,131 + BWI76_RS15540 0.21 -0.8 3,256,328 + BWI76_RS15540 0.62 -1.9 3,256,375 - BWI76_RS15540 0.72 -0.4 3,256,428 + BWI76_RS15540 0.83 -0.1 3,256,433 + BWI76_RS15540 0.84 -1.9 3,256,433 + BWI76_RS15540 0.84 +0.6 3,256,441 - BWI76_RS15540 0.86 -0.6 3,256,450 + BWI76_RS15540 0.88 -1.0 3,256,623 - -1.6 3,256,664 + BWI76_RS15545 0.14 -2.5 3,256,693 + BWI76_RS15545 0.24 -0.2 3,256,708 - BWI76_RS15545 0.29 +0.1 3,256,796 + BWI76_RS15545 0.60 -0.0 3,256,836 + BWI76_RS15545 0.74 +0.4 3,256,944 + +0.7 3,257,091 - +0.1 3,257,216 - -0.5 3,257,334 + BWI76_RS15550 0.14 +0.4 3,257,404 - BWI76_RS15550 0.17 -0.4 3,257,587 + BWI76_RS15550 0.24 +0.2 3,257,632 - BWI76_RS15550 0.26 -0.4 3,257,632 - BWI76_RS15550 0.26 -2.2 3,257,697 - BWI76_RS15550 0.28 -0.6 3,258,102 - BWI76_RS15550 0.43 -1.9 3,258,148 + BWI76_RS15550 0.45 -0.6 3,258,510 + BWI76_RS15550 0.59 -1.6 3,258,621 - BWI76_RS15550 0.63 -2.2 3,258,627 + +2.2 3,258,663 - BWI76_RS15550 0.64 -0.7 3,258,663 - BWI76_RS15550 0.64 -3.5 3,258,729 + BWI76_RS15550 0.67 -0.3 3,258,729 + BWI76_RS15550 0.67 +0.7 3,258,860 + BWI76_RS15550 0.72 +1.2 3,258,873 + BWI76_RS15550 0.72 -0.8 3,259,002 + BWI76_RS15550 0.77 -0.1 3,259,088 + BWI76_RS15550 0.80 -1.1 3,259,106 - BWI76_RS15550 0.81 -1.3 3,259,151 - BWI76_RS15550 0.83 -0.2 3,259,174 - BWI76_RS15550 0.84 -0.8 3,259,255 + BWI76_RS15550 0.87 -2.0 3,259,333 + BWI76_RS15550 0.89 -0.6 3,259,362 + -0.9 3,259,362 + -2.2 3,259,370 - -0.5 3,259,370 - -1.5 3,259,370 - -1.5 3,259,390 + +0.4 3,259,399 - -0.2 3,259,414 - +0.6 3,259,418 + -0.1 3,259,425 + +0.9 3,259,426 - -1.7 3,259,449 + -0.4 3,259,449 + -0.4 3,259,457 - -0.2 3,259,457 - -0.6 3,259,457 - +0.2 3,259,480 + -0.9 3,259,535 + -0.6 3,259,535 + -1.3 3,259,535 + +0.4 3,259,543 - +0.3 3,259,606 + -1.9 3,259,607 + +1.2 3,259,608 + +0.0 3,259,608 + -0.2 3,259,689 + -2.2 3,259,708 - -0.5 3,259,749 + +1.4 3,259,757 - BWI76_RS15555 0.11 -0.3 3,259,757 - BWI76_RS15555 0.11 -0.9 3,259,757 - BWI76_RS15555 0.11 -0.1 3,259,757 - BWI76_RS15555 0.11 -1.1 3,259,757 - BWI76_RS15555 0.11 -3.0 3,259,765 - BWI76_RS15555 0.13 -1.0 3,259,776 - BWI76_RS15555 0.15 -1.3 3,259,776 - BWI76_RS15555 0.15 -0.4 3,259,790 - BWI76_RS15555 0.18 -0.8 3,259,848 - BWI76_RS15555 0.31 +0.4 3,259,889 - BWI76_RS15555 0.39 -0.2 3,259,891 + BWI76_RS15555 0.40 -2.7 3,259,901 - BWI76_RS15555 0.42 +0.1 3,259,935 - BWI76_RS15555 0.49 -0.2 3,259,985 - BWI76_RS15555 0.60 -1.2 3,260,007 - BWI76_RS15555 0.64 +0.0 3,260,007 - BWI76_RS15555 0.64 -3.5 3,260,024 - BWI76_RS15555 0.68 -2.2 3,260,024 - BWI76_RS15555 0.68 -0.3 3,260,043 - BWI76_RS15555 0.72 -2.4 3,260,069 + BWI76_RS15555 0.77 -1.4 3,260,077 - BWI76_RS15555 0.79 -0.9 3,260,077 - BWI76_RS15555 0.79 -1.2 3,260,184 - -0.6 3,260,184 - -1.1 3,260,241 - BWI76_RS15560 0.21 -2.5 3,260,248 + BWI76_RS15560 0.23 -2.1 3,260,256 - BWI76_RS15560 0.26 +0.1 3,260,256 - BWI76_RS15560 0.26 -1.5 3,260,267 - BWI76_RS15560 0.29 -1.7 3,260,269 + BWI76_RS15560 0.29 -0.9 3,260,311 - BWI76_RS15560 0.42 -2.4 3,260,339 + BWI76_RS15560 0.50 +0.6 3,260,339 + BWI76_RS15560 0.50 -0.1 3,260,339 + BWI76_RS15560 0.50 +0.2 3,260,339 + BWI76_RS15560 0.50 -0.9 3,260,339 + BWI76_RS15560 0.50 -1.5 3,260,339 + BWI76_RS15560 0.50 -0.4 3,260,347 - BWI76_RS15560 0.53 -2.6 3,260,347 - BWI76_RS15560 0.53 -0.4 3,260,347 - BWI76_RS15560 0.53 -0.2 3,260,347 - BWI76_RS15560 0.53 -1.8 3,260,347 - BWI76_RS15560 0.53 -0.6 3,260,347 - BWI76_RS15560 0.53 -0.5 3,260,348 - BWI76_RS15560 0.53 -1.6 3,260,496 - -0.7 3,260,552 - -0.3 3,260,583 - -2.4
Or see this region's nucleotide sequence